Sustratos cromogénicos
Los sustratos cromogénicos son compuestos que producen un cambio de color visible cuando son metabolizados por enzimas específicas. Estos sustratos se utilizan ampliamente en ensayos diagnósticos, investigaciones bioquímicas y ensayos de inmunoabsorción enzimática (ELISA) para detectar y cuantificar la actividad enzimática. El cambio de color permite una identificación visual rápida y sencilla de las reacciones enzimáticas, lo que convierte a los sustratos cromogénicos en herramientas esenciales en el análisis de laboratorio. En CymitQuimica, ofrecemos una amplia selección de sustratos cromogénicos de alta calidad para apoyar sus aplicaciones de investigación y diagnóstico.
Productos de "Sustratos cromogénicos"
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Aldol® 515 acetate, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol® 515 acetate is a chromogenic and fluorogenic substrate, used for detection of esterase activity. The colorless product is cleaved by purified esterase, or esterase secreted by microorganisms in liquid or solid media, yielding a red color and red fluorescence signal when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 acetate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of a purified esterase, color and fluorescence develops within minutes to few hours.Fórmula:C25H22N2O3Pureza:Min. 95.0 Area-%Peso molecular:398.45 g/molAldol® 515 L-proline amide, hydrochloride, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol® 515 L-proline amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-proline aminopeptidase activity. The colorless enzyme substrate is cleaved by L-proline aminopeptidase produced by microorganisms grown in liquid or solid media, yielding red-orange color. In addition, red fluorescence signals are generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-proline amide is suitable for use under aerobic and anaerobic conditions.Fórmula:C28H29ClN4O2Pureza:Min. 95 Area-%Forma y color:PowderPeso molecular:489.02 g/molRef: 3D-EA175375
Producto descatalogado4-Chloro-2-nitrophenyl b-D-galactopyranoside
4-Chloro-2-nitrophenyl b-D-galactopyranoside is a fluorogenic substrate that is used in diagnostic testing, such as food and environmental testing. 4-Chloro-2-nitrophenyl b-D-galactopyranoside is an enzyme substrate that can be used to measure the activity of beta galactosidase. This substrate is also chromogenic, which means that it will change color when exposed to the enzyme. 4CNPGalA has been shown to be an excellent ligand for beta galactosidase and can be used in conjugation reactions with other molecules.Pureza:Min. 95%Peso molecular:335.69 g/mol6-Chloro-3-indoxyl-β-D-glucuronic acid methyl ester, Biosynth Patent: EP 2718458 and US 9127303
6-Chloro-3-indoxyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate that can be used for the detection of indoxyl glucuronide in urine and other biological fluids. 6-Chloro-3-indoxyl-beta-D-glucuronic acid methyl ester is a white or light yellow crystalline solid that is soluble in water. It has a melting point of about 80°C and is stable at pH levels ranging from 2 to 12. The CAS number for this compound is 13982-87-6.Fórmula:C15H16ClNO7Pureza:Min. 97.0 Area-%Peso molecular:357.74 g/mol4-Nitrophenyl b-D-cellopentaoside
CAS:Substrates for endoglucanase and beta-cellobiohydrolase activity in the study of digestive system in Zygentoma as an insect model for high cellulase activityFórmula:C36H55NO28Pureza:Min. 95%Forma y color:PowderPeso molecular:949.81 g/mol2,4-Dinitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside
2,4-Dinitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate for use in diagnostic testing. It reacts with the enzyme β -galactosidase to produce a blue color. This product is used as an indicator of β -galactosidase activity in bacterial cultures or as an indicator of the presence of this enzyme in food or other materials. It can be used to detect and measure the level of β -galactosidase in a sample. The reaction can be monitored by measuring light emission at 450 nm. 2,4-Dinitrophenyl 2 acetamido 2 deoxy b D glucopyranoside has a CAS number of 47868-87-0 and is available in high purity (>97%).Pureza:Min. 95%Peso molecular:387.31 g/mol4-Nitrophenyl b-D-cellohexaoside
CAS:4-Nitrophenyl b-D-cellohexaoside is a substrate for many enzymes and can be used to detect the presence of these enzymes in biological samples. It can also be used to detect the presence of bacteria, fungi, parasites, and viruses. 4-Nitrophenyl b-D-cellohexaoside is an excellent substrate for enzyme reactions that produce a chromogenic or fluorogenic product. This product is often conjugated with other compounds such as antibodies or antigens to form an immunoassay. 4-Nitrophenyl b-D-cellohexaoside is made from a high purity chemical by a process called nitration with sodium nitrate and sulfuric acid.Pureza:Min. 95%Peso molecular:1,111.95 g/mol4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside
4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside is an enzyme substrate that is used in the detection of phenylalanine, tyrosine, and tryptophan. It is also used for environmental testing, bioluminescence, culture media, chemiluminescence, fluorogenic substrate, food testing, diagnostics and other applications.Pureza:Min. 95%Forma y color:Off-White PowderPeso molecular:545.49 g/mol4-Nitrophenyl 6-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside
4-Nitrophenyl 6-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that is used in assays for detecting nitrates. This compound reacts with 4-(methylnitrosamino)pyridine (MNAP) to produce an intense light that can be detected by a luminometer. It has been shown to react with the enzyme nitrate reductase and is used in food testing and environmental testing as a means of measuring the amount of nitrates present.Pureza:Min. 95%Peso molecular:925.84 g/mol2-Nitrophenyl b-D-cellobioside
CAS:Chromogenic substrate targeting cellulasesFórmula:C18H25NO14Pureza:Min. 98 Area-%Forma y color:White PowderPeso molecular:463.39 g/molRef: 3D-EN03286
Producto descatalogado2-Nitrophenyl b-L-fucopyranoside
CAS:2-Nitrophenyl beta-L-fucopyranoside is a chromogenic substrate used to detect the enzymatic activity of beta-L-fucosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl beta-L-fucopyranoside is used in bacterial fucose metabolism studies to identify and quantify the enzymatic activity in, for example, Bacteroides spp..Pureza:Min. 98 Area-%Forma y color:PowderPeso molecular:285.25 g/mol2-Fluoro-4-nitrophenyl b-D-galactopyranoside
CAS:2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.Pureza:Min. 95%Forma y color:PowderPeso molecular:319.24 g/mol4-Nitrophenyl-beta-D-cellobioside
CAS:Chromogenic substrate for β-D-Cellobiosidase yielding a yellow solution upon cleavage.Fórmula:C18H25NO13Pureza:Min. 98 Area-%Peso molecular:463.39 g/mol2-Chloro-4-nitrophenyl-β-D-cellotetraoside
CAS:2-chloro-4-nitrophenyl-beta-D-cellotetraoside is a chromogenic substrate for measuring cellulase activity and other glycosyl hydrolases. The release of 2-chloro-4-nitrophenol can be detected at 405 nm.Fórmula:C30H44ClNO23Pureza:Min. 95%Forma y color:PowderPeso molecular:822.12 g/mol4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(b-D-galactopyranosyl)-b-D-glucopyranoside
CAS:Chromogenic substrate for fucosyltransferasesFórmula:C20H28N2O13Pureza:Min. 98 Area-%Forma y color:White PowderPeso molecular:504.44 g/mol1-Naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside
CAS:1-Naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate commonly used to assay the activity of enzymes that cleave N-acetyl-beta-D-glucosaminide, such as beta-N-acetylglucosaminidase. Upon cleavage by the enzyme of interest, the substrate releases a yellow-colored 1-naphthylamine, which can be measured spectrophotometrically. This substrate is widely used in biomedical research and drug discovery for studying lysosomal storage disorders, bacterial infections, and neurological diseases.Pureza:Min. 95%Peso molecular:347.36 g/molAldol® 515 phosphate, disodium salt, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol® 515 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 515 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 515 phosphate can be used under aerobic and anaerobic conditions.Fórmula:C23H19N2Na2O5PPureza:Min. 95%Forma y color:PowderPeso molecular:480.36 g/molRef: 3D-EA175362
Producto descatalogadoAldol® 515 acetate, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol® 515 acetate is a chromogenic and fluorogenic substrate, used for detection of esterase activity. The colorless product is cleaved by purified esterase, or esterase secreted by microorganisms in liquid or solid media, yielding a red color and red fluorescence signal when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 acetate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of a purified esterase, color and fluorescence develops within minutes to few hours.Fórmula:C25H22N2O3Pureza:Min. 95%Forma y color:PowderPeso molecular:398.45 g/mol4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-6-O-benzoyl-3,4-O-isopropylidene-a-D-galactopyranoside
CAS:4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-6-O-benzoyl-3,4-O-isopropylidene a D galactopyranoside is an analog of galactose that binds to the DNA and regulates gene expression. This compound has been shown to be able to regulate gene expression levels in cells. 4NP2B6OB was used as a probe for microarray analysis of gene expression profiles from environmental samples. It was found that 4NP2B6OB could be used as a probe for the identification of genes in high density arrays.Pureza:Min. 95%Peso molecular:717.67 g/mol4-Nitrophenyl b-D-cellobioside heptaacetate
4-Nitrophenyl b-D-cellobioside heptaacetate is a chromogenic substrate for enzymes that catalyze the hydrolysis of glycosidic bonds. This product is used as a diagnostic tool for identifying and characterizing glycosidases and other enzymes that degrade glycosidic bonds. 4-Nitrophenyl b-D-cellobioside heptaacetate is also used in culture media to measure the activity of cellulase enzymes by measuring the release of reducing sugars. The color change of this substrate can be detected at 405 nm, 490 nm, or 550 nm.Pureza:Min. 95%Peso molecular:757.65 g/mol
