Sustratos cromogénicos

Aldol® 467 N-acetyl-beta-D-glucosaminide is a chromogenic and fluorogenic enzyme substrate used for detection of beta-N-acetyl-glucosaminidase activity. The colorless enzyme substrate is cleaved in the presence of beta-N-acetyl-glucosaminidase yielding development of a yellow product. In addition, green fluorescence is generated when a suitable matrix or Aldol® 355 fluorescence enhancer is present. Aldol® 467 N-acetyl-beta-D-glucosaminide amide can be used in enzymatic assays or in live bacterial cultures. Aldol® 467 N-acetyl-beta-D-glucosaminide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Chromogenic substrate for beta-glucanase

Naphthol AS-BI N-acetyl-beta-D-glucosaminide is a fluorogenic substrate for the pararosaniline-glyoxalase (PG) reaction. This substrate is used to study enzymatic activity in two human isoenzymes of PG, PG I and PG II. The conversion of this substrate by PG I or PG II results in a color change from yellow to red. Naphthol AS-BI N-acetyl-beta-D-glucosaminide may be used as an experimental tool to measure the activity of these enzymes and to study the effect of inhibitors on their activity. It can also be used as a marker for studying enzyme transfer between cells.

Aldol® 515 palmitate is a high quality, non-toxic and water soluble chromogenic substrate for enzymes. It is used to detect the presence of lipase activity due to yielding a red color.

Monohydrate; beta-glucuronidase substrate; yellow/pink color Beta-glucuronidase activity can be measured in a colorimetric assay with phenolphthalein beta-D-glucuronide as substrate. Starting assay conditions can include 30 µg of enzyme-containing material, 0.6 mM phenolphthalein beta-D-glucuronide in 100 mM sodium phosphate buffer in a total volume of 100 µL and monitoring absorbance changes at 540 nm. Temperature incubation can be used to promote the enzymatic activity (Galindo, 2012).

2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used to detect and measure the activity of glycosidase enzymes. Glycosidases are enzymes that hydrolyze glycosidic bonds in carbohydrates, and are produced by many organisms. The substrate is cleaved by glycosidase to release a chromophore, which can be quantified via spectrophotometry analysis. 2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the detection of NAGase activity in biological samples, such as serum, urine, and cell lysates. It is also used for the identification and characterization of glycosidases in microorganisms and the study of glycosylation-related processes in cells and tissues.

N-a-Boc-N-epsilon-cbz-L-lysine 4-nitroanilide is a compound that has been shown to regulate the production of angiotensin. It also acts as an inhibitor for the production of fibronectin, which is key in the regulation of inflammation and wound healing. N-a-Boc-N-epsilon-cbz-L-lysine 4 nitroanilide has been shown to be a trifunctional molecule and can yield three different products: oxychlorides, hydrated and solvates. This molecule also regulates phosphorus levels. There are many functions that this molecule performs, including binding with amino acids and regulating their function, which is why it is so important to study this molecule more closely.

4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chromogenic pNP substrate specifically designed for the analysis of N-acetylglucosaminidase activity. Upon enzyme action, the substrate releases 4-nitrophenol, a yellow compound that can be detected by spectrophotometric methods, providing a reliable and sensitive means of quantifying enzyme activity. This versatile substrate is widely used in biochemical studies, disease diagnostics, and the enzyme production industry.

5-Bromo-3-indolyl-a-D-galactopyranoside (5BIG) is a bioluminescent substrate that is used in various applications, including environmental testing, diagnostics, and the detection of bacterial activity. 5BIG is a ligand for luciferase enzymes and is used as a chromogenic substrate. It also has the ability to be conjugated with other molecules for use in enzyme substrates or fluorescence assays. 5BIG has been shown to be an effective fluorogenic substrate for enzymes such as alkaline phosphatase and beta-glucuronidase.

2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid ammonium salt is a chromogenic substrate commonly used in the detection and quantification of sialidase activity. Sialidases are a family of enzymes that hydrolyze sialic acid residues from glycoconjugates. This substrate is used to detect bacterial and viral sialidases, which play an important role in pathogen infectivity. The ammonium salt form of the substrate is preferred for its solubility in water-based assay systems.

Aldol 535 is a chemiluminescent substrate for the detection of myo-inositol-1-phosphate. It has been shown to have high purity and high quality, and is suitable for use in diagnostics, food testing, and as a bioluminescence substrate. Aldol 535 is also used as a ligand in various enzyme assays. It has been tested for its ability to react with the fluorogenic substrates: 4-methylumbelliferyl phosphate and 4-methylumbelliferone phosphate. The CAS number is 2484872-80-0.

6-Bromo-2-naphthyl-b-D-xylopyranoside (BNX) is a chromogenic enzyme substrate used primarily to detect xylosidase activity. It is cleaved by the enzyme, resulting in the release of a colored product that can be measured spectrophotometrically. BNX is widely used as a substrate in research studies for the detection and quantification of various glycoside hydrolases, and it is particularly useful for studying cellulases and xylanases.

Aldol® 470 phosphate, disodium salt is a fluorogenic substrate that is used in the detection of enzyme activity. It can be used to detect the presence of an enzyme by measuring the light emitted from the substrate after it has been converted to fluorescent product. Aldol® 470 phosphate, disodium salt is a chromogenic substrate that is used for detecting and measuring enzyme activity. The product of this reaction can be measured by absorbance or fluorescence spectroscopy, making it useful for many different types of diagnostic tests. This product can be conjugated with a variety of ligands for use in diagnostic assays and research. Aldol® 470 phosphate, disodium salt can also be used in culture media as a bioluminescent substrate for bacterial growth and metabolism studies. This product can also be used as an environmental testing reagent or as a staining agent in microscopy applications.

Water soluble, chromogenic substrate for visualizing alkaline phosphatase activity. Produces a blue insoluble end product that is detected visually when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situhybridization, ELISAs and immunohistochemistry.

2-Nitrophenyl-beta-D-galactopyranoside is a non-animal origin chromogenic substrate for the detection of beta-galactosidase activity. It is used in food testing, diagnostics, and environmental testing. 2-Nitrophenyl-beta-D-galactopyranoside is also used as a ligand in enzyme assays and a fluorogenic substrate for chemiluminescent reactions. This product is conjugated to HRP.

5-Iodo-3-indolyl b-D-galactopyranoside is a chromogenic substrate used for beta-galactosidase. It is incorporated into media or detection kits for rapid and direct identification of microorganisms.

b-Naphthyl-b-maltotrioside is a fluorogenic substrate that is used in enzymatic and chromogenic assays. It is a bifunctional substrate that reacts with enzymes to produce light. b-Naphthyl-b-maltotrioside can be used as a conjugate for immunoassays, such as ELISA. This substrate is also suitable for use in diagnostics, food testing, and environmental testing.

4-Nitrophenyl 2,3,4-tri-O-acetyl-6-O-trityl-a-D-glucopyranoside is a substrate for chemiluminescent assays. It is a high quality chemical that can be used in environmental testing, staining, ligand binding and bioluminescence. 4NP2T6AODG is used as a chromogenic substrate or fluorogenic substrate to detect the presence of enzymes in diagnostics and food testing. This product has CAS No. 67794-14-0 and can be purchased at high purity.

1-Naphthyl a-D-galactopyranoside is a chromogenic substrate use to detect alpha-galactosidase activity.

4-Nitrophenyl-alpha-D-glucopyranoside is a chemical compound that belongs to the class of nitrophenols. It is an anti-infective agent and has been used as a substrate in vitro for the study of enzyme activities. 4-Nitrophenyl-alpha-D-glucopyranoside has been shown to inhibit the growth of colorectal adenocarcinoma cells, both in vivo and in vitro, at low concentrations. This compound also inhibits bacterial growth by blocking the synthesis of proteins and nucleic acids. When ingested, 4-Nitrophenyl-alpha-D-glucopyranoside can be hydrolyzed into nitrite, which is toxic to bacteria.