Sustratos cromogénicos

4-Nitrophenyl 2-O-(a-L-fucopyranosyl)-a-D-galactopyranoside is a chromogenic pNP enzyme substrate consisting of a fucose and galactose moieties. Upon enzymatic hydrolysis by specific fucosidases or galactosidases, it releases the highly chromogenic molecule 4-nitrophenol, which exhibits a distinct yellow color that can be monitored spectrophotometrically. This substrate is frequently used to study enzyme specificity, kinetics, and inhibition, and it plays a critical role in the investigation of carbohydrate active enzymes (CAZymes), the screening of enzyme inhibitors, and the development of novel analytical methods for the detection and quantitation of enzyme activities.

4-Nitrophenyl-N-acetyl-α-D-glucosaminide is a chromogenic substrate for N-acetyl-β-D-glucosaminidase yielding a yellow solution upon cleavage.

4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-6)-b-D-galactopyranosyl-(1-4)-2-acetamido-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate used in the detection of sialidase activity. It is also used as a chromogenic substrate in the determination of β -galactosidase activity.

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-a-D-mannopyranoside is a fluorogenic substrate that is used as a diagnostic tool and can be used to detect the presence of β--glucosidase in the gut. It is also used as a conjugate with enzymes such as peroxidase or alkaline phosphatase for use in enzyme catalyzed reactions. The product has been shown to be active against a variety of bacteria, including Staphylococcus aureus and Escherichia coli. This product has been shown to produce chemiluminescence when incubated with horseradish peroxidase and hydrogen peroxide. This product is light sensitive and should be stored at room temperature away from direct sunlight.

Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol substrates work not only under aerobic but also under anaerobic conditions

4-Nitrophenyl a-D-maltopentaoside serves as a substrate for enzymes functioning in carbohydrate metabolism, including α-amylase and maltase. Upon enzymatic hydrolysis, it yields a yellow 4-nitrophenolate product that can be easily detected and monitored spectrophotometrically. 4-Nitrophenyl a-D-maltopentaoside . This substrate is ideal for research purposes in fields such as biochemistry, enzymology, and glycobiology, providing an efficient tool for characterizing maltopentaoside-related enzymes and understanding their mechanisms of action.

Chromogenic substrate for alkaline phosphatase

Aldol® 467 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 467 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 467 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranosyl)-3,6-di-O -acetyl2 deoxy b D glucopyranosid e is a chemiluminescence substrate that reacts with hydrogen peroxide to produce light. It is used in diagnostics for the detection of bacterial and fungal cultures. The reaction between 4NPAA and hydrogen peroxide generates light at 540 nm. This product has been tested for purity and quality using various methods including culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, enzyme substrate, food testing and conjugate.

Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color

Aldol® 470 nonanoate is a chemiluminescent substrate that can be used as a ligand for the detection of C8/C9-esterases, lipases activity, to result in an orange/green color. Aldol® 470 nonanoate has been shown to be active with horseradish peroxidase in a number of different assay systems. The purity and quality of Aldol® 470 nonanoate has been tested with culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, chromogenic substrate, enzyme substrate, food testing and conjugates.

Aldol 518 Palmitate is a high purity and high quality, bioluminescent substrate for use in diagnostic applications. Aldol 518 Palmitate is an enzyme substrate that is used for the detection of hydrogen peroxide through chemiluminescence or bioluminescence. It can be used in the diagnosis of bacterial infections and to test food products for spoilage. The enzyme substrate has a CAS number of 646-12-2.

Remazol Brilliant Blue R D-xylan is a chromogenic substrate used for the detection and quantification of xylanase enzyme activity. This substrate consists of a xylan polymer backbone covalently linked to the Remazol Brilliant Blue R dye. Upon cleavage by xylanase enzymes, the dye is released, producing a vibrant blue color that can be easily measured spectrophotometrically. This substrate is widely used in various applications, including the study of xylanase-producing microorganisms, optimization of enzyme production, and evaluation of enzyme kinetics.

2-Naphthyl-β-D-galactopyranoside is a substrate for β-galactosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

Aldol® 495 nonanoate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C9 esterases, yielding development of an orange coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 495 nonanoate can be used in enzyme assays and live bacterial cultures. Aldol® 495 nonanoate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is a useful substrate for enzymatic assays. This high-sensitivity chromogenic pNP substrate offers enhanced selectivity and increased detection ability, allowing for a broad range of applications in the field of glycosidase research. Featuring rapid colorimetric readouts with easily detectable endpoint determination, this substrate delivers clear and accurate results every time.

2-Chloro-4-nitrophenyl beta-D-maltotrioside, often abbreviated as CNP-G3, is a chromogenic substrate to detect and mesaure alpha-amylase activity. Evaluation and monitoring of alpha-amylase activity is important to help diagnose certain diseases such as pancreatitis.

2,4-Dinitrophenyl 2-deoxy-2-fluoro-β-D-glucopyranoside, sometimes referred as 2F-DNPG, is an enzyme substrate for measuring beta-glucosidase activity. It is a mechanism-based inactivator of glycosidases, including beta-glucocerebrosidase (Gcase), a lysosomal enzyme encoded by the GBA1 gene responsible for breaking down glucosylceramide into ceramide and glucose. The use of 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-D-glucopyranoside as an enzyme substrate permits the study of the catalytic mechanism and reaction intermediate of these enzymes.

Chromogenic β-maltosidase substrate yielding a yellow solution upon cleavage.

4-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside is a chromogenic substrate for alpha-N-acetylgalactosaminidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside is used in different applications such as the diagnosis of Schindler-Kanzaki disease and other lysosomal storage disorders.