Substrats fluorogènes
Dans cette catégorie, vous trouverez une sélection complète de substrats fluorogènes conçus pour la catalyse enzymatique. Ces substrats sont utilisés avec divers types d'enzymes et sont capables de produire de la couleur et de la fluorescence lors de la réaction. Les substrats fluorogènes sont des outils essentiels dans la recherche en biochimie et biologie moléculaire, permettant la détection et la quantification de l'activité enzymatique avec une grande sensibilité et spécificité. Ils sont largement utilisés dans les essais pour étudier la cinétique enzymatique, rechercher des inhibiteurs d'enzymes et surveiller les processus cellulaires. Chez CymitQuimica, nous proposons des substrats fluorogènes de haute qualité pour soutenir vos recherches, garantissant des résultats fiables et précis dans vos essais enzymatiques.
Produits appartenant à la catégorie "Substrats fluorogènes"
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4-Methylumbelliferyl 4-deoxychitobiose
CAS :4-Methylumbelliferyl 4-deoxychitobiose is a fluorescent substrate used to detect the activity of chitinase. It is a chromogenic substrate that can be used in diagnostic applications and provides a fluorescence response upon hydrolysis. This product is highly pure, with a minimum of 98% purity. It can be used as an enzyme substrate for the detection of chitinase activity or as a ligand for immunoassays.Degré de pureté :Min. 95%Masse moléculaire :566.55 g/mol(+)-Biotin 4-amidobenzoic acid sodium salt
CAS :(+)-Biotin 4-amidobenzoic acid sodium salt, often referred as (+)-biotin PABA, is a fluorogenic substrate used to measure biotinidase activity, especially in newborn screening programs.Formule :C17H20N3NaO4SDegré de pureté :Min. 95%Couleur et forme :White Slightly Yellow PowderMasse moléculaire :385.41 g/mol4-Methylumbelliferyl sulfate, potassium salt
CAS :4-Methylumbelliferyl sulfate, potassium salt is a metabolite of 4-methylumbelliferone and has been used as a human serum marker. This molecule is conjugated with glucuronide and p-hydroxybenzoic acid to form the sulfate, glucuronide conjugate. The reaction mechanism of this compound is not known, but it is thought that the metabolism may involve oxidation at the methyl group. The analytical method for this compound can be performed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This method can also be used in vitro to test antimicrobial activity against bacteria such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.Formule :C10H7KO6SDegré de pureté :Min. 99.0 Area-%Masse moléculaire :294.33 g/mol6,8-Difluoro-4-methylumbelliferyl b-D-glucopyranoside
CAS :6,8-Difluoro-4-methylumbelliferyl b-D-glucopyranoside is a versatile fluorogenic substrate suitable for the measurement of various glycosidase enzymes. Its unique structure allows for enhanced sensitivity and selectivity in enzyme assays, making it ideal for studying enzyme kinetics and screening of potential enzyme inhibitors.Degré de pureté :Min. 95%Masse moléculaire :374.29 g/molBoc-Val-Pro-Arg-PAB-Resorufin
Boc-Val-Pro-Arg-PAB-Resorufin is fluorogenic substrate for thrombin and the thrombin-staphylocoagulase complex.Formule :C40H50N8O8Degré de pureté :Min. 85 Area-%Masse moléculaire :770.87 g/molBis-(4-methylumbelliferyl)phosphate
CAS :Bis-(4-methylumbelliferyl)phosphate, or bis-4MU-phopshate, is a fluorogenic substrate used to study phosphatase activity, particularly in enzyme-linked immunosorbent assays (ELISAs).Formule :C20H15O8PDegré de pureté :Min. 95 Area-%Couleur et forme :White PowderMasse moléculaire :414.3 g/mol6-(b-D-Galactopyranosyloxy)-9-(4-methoxy-2-methylphenyl)-3H-xanthen-3-one
CAS :6-(b-D-Galactopyranosyloxy)-9-(4-methoxy-2-methylphenyl)-3H-xanthen-3-one (MGBG) is a hydrophilic activatable fluorescence probe that has been shown to be effective in photodynamic therapy. MGBG is activated by light and has a high affinity for esterases, which are found in many tissues. The fluorescence of the MGBG is quenched by the esterase enzymes, but when it is exposed to light and oxygen, the probe becomes activated and its fluorescence increases. MGBG can be used as a fluorescent probe for intraperitoneal tumor resection or as a fluorescent imaging agent in optical microscopy.Degré de pureté :Min. 95%Masse moléculaire :494.49 g/molN-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride
CAS :N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a Ffluorogenic substrate used in protease activity assays.Formule :C24H27N5O5·ClHDegré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :501.96 g/mol4-Methylumbelliferyl acetate - 95%
CAS :4-Methylumbelliferyl acetate is a fluorescent compound that can be used to measure the activity of cytochrome P450 enzymes. The fluorescence intensity of 4-methylumbelliferyl acetate increases when it reacts with substrates and inhibitors in vitro. This chemical has been shown to inhibit the enzyme cyclooxygenase in rat liver microsomes, which may lead to blood disorders. It also inhibits coumarin derivatives and human pathogens, such as erythromycin-resistant Staphylococcus aureus (MRSA). 4-Methylumbelliferyl acetate is used as a substrate for polymerase chain reaction (PCR) analysis because it is not susceptible to hydrolysis by esterases.Formule :C12H10O4Degré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :218.21 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-4-O-(a-L-fucopyranosyl)-b-D-glucopyranoside
CAS :4-Methylumbelliferyl 2-acetamido-2-deoxy-4-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-4-O-(alpha-L-fucopyranosyl)-beta-D-glucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis (deficiency of the enzyme alpha-L-fucosidase) and glycoprotein metabolism disorder detection.Formule :C24H31NO12Degré de pureté :Min. 95 Area-%Couleur et forme :PowderMasse moléculaire :525.5 g/mol4-Methylumbelliferyl 4,6-O-benzylidene-b-D-galactopyranoside
4-Methylumbelliferyl 4,6-O-benzylidene-b-D-galactopyranoside is a fluorogenic substrate that is used in the detection of galactosidases. It has been shown to be a potent inhibitor of these enzymes and can be used to study the activity of various galactosidases. This substrate is used in the measurement of carbohydrate metabolism, including glycolysis and glycogenolysis. 4-Methylumbelliferyl 4,6-O-benzylidene-b-D-galactopyranoside has been shown to produce chemiluminescence when reacted with horseradish peroxidase and hydrogen peroxide. It also produces bioluminescence when exposed to luciferase and adenosine triphosphate (ATP).Degré de pureté :Min. 95%Masse moléculaire :426.42 g/mol4-Methylumbelliferyl a-L-idopyranosiduronic acid
CAS :4-Methylumbelliferyl a-L-idopyranosiduronic acid is an ester that is used as a marker for the identification of glycolipids. It is a fluorogenic substrate for alpha-L-iduronidase used as a marker for the diagnosis of autoimmune diseases, including diabetic neuropathy. 4-Methylumbelliferyl a-L-idopyranosiduronic acid has been shown to inhibit inflammation and cancer, although it is not currently approved for this use. The mechanism by which 4-methylumbelliferyl a-L-idopyranosiduronic acid inhibits inflammation may be due to its ability to block cation channels on the surface of cells, thereby preventing inflammatory mediators from entering cells.Formule :C16H16O9Degré de pureté :Min. 97 Area-%Couleur et forme :White Off-White PowderMasse moléculaire :352.29 g/molResorufin oleate
Resorufin oleate is a fluorogenic lipase substrate.Degré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :477.64 g/mol6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside
6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside is a fluorescent substrate that is used to measure the activity of the enzyme beta-glucuronidase. It is a chemiluminescent compound that emits light when hydrolyzed by an enzyme. 6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotetraoside has been shown to be a high quality and environmentally friendly product, as it does not cause any significant change in pH or turbidity in water samples. It has also been shown to be nontoxic and nonmutagenic at concentrations up to 400 ppm. This product can be used for a variety of purposes, including diagnostic tests for bacterial infections or food testing.Degré de pureté :Min. 95%Masse moléculaire :913.15 g/molL-Arginyl-L-arginine 7-amido-4-methylcoumarin trihydrochloride
CAS :Fluorogenic substrate for cathepsin B or aminopeptidase III. Yields a blue fluorescent solution upon cleavage.Formule :C22H36Cl3N9O4Degré de pureté :Min. 98 Area-%Masse moléculaire :596.95 g/mol4-Methylumbelliferyl 2-deoxy-2-sulfamino-b-D-glucopyranoside sodium salt
4-Methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium salt is a fluorogenic substrate for N-sulfoglucosamine sulfohydrolase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium saltas a substrate for measuring the N-sulfoglucosamine sulfohydrolase activity is used for screening of Mucopolysaccharidosis IIIA (MPS IIIA) and diagnosis of Sanfilippo A syndrome.Formule :C16H19NO10SNaDegré de pureté :Min. 95%Couleur et forme :PowderMasse moléculaire :439.37 g/molL-Alanine-7-amido-4-methylcoumarin hydrochloride
CAS :L-Alanine-7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for alanine aminopeptidase.Formule :C13H14N2O3·HClDegré de pureté :Min. 90%Couleur et forme :PowderMasse moléculaire :282.72 g/mol4-Methylumbelliferone
CAS :4-Methylumbelliferone is a coumarin derivative that inhibits the activity of enzymes such as p-nitrophenyl phosphate oxidase and aminopeptidase. It is used in vitro to study oxidative injury, which plays a role in the pathogenesis of inflammatory diseases, including primary sclerosing cholangitis and bone marrow transplantation. 4-Methylumbelliferone has been shown to induce apoptosis by increasing reactive oxygen species levels and activating toll-like receptor 4 (TLR4). It also has inhibitory effects on osteosarcoma cell proliferation in vitro.Formule :C10H8O3Masse moléculaire :176.17 g/mol4-Methylumbelliferyl phosphate, free acid
CAS :A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.Formule :C10H9O6PMasse moléculaire :256.15 g/mol4-(Trifluoromethyl)umbelliferyl-b-D-cellotrioside
CAS :4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is a fluorogenic substrate for cellulase. After cleavage, the fluorophore 4-(trifluoromethyl)umbelliferone, commonly referred as TFMU, is released, exhibiting a blueish color. 4-(Trifluoromethyl)umbelliferyl-beta-D-cellotrioside is used to study the enzyme kinetics in bacterial cellulose degradation assays as well as in biomass conversion research studies.Degré de pureté :Min. 95%Couleur et forme :White to off-white solid.Masse moléculaire :716.56 g/mol
