Neuroscience

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CAST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CAST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CAST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CAST in the samples is then determined by comparing the OD of the samples to the standard curve.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human EM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EM2 in the samples is then determined by comparing the OD of the samples to the standard curve.

ETAP, a dual inhibitor of both monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B), exhibits antidepressant-like effects and is utilized in the research

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5HTR2C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5HTR2C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5HTR2C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5HTR2C in the samples is then determined by comparing the OD of the samples to the standard curve.

GSNKGAIIGLM(131602-53-4(free base)) (Amyloid beta-peptide(25-35)) is the fragment Aβ(25-35) of the Alzheimer's amyloid β-peptide.

Nitroxazepine (CIBA 2330Go), a tricyclic antidepressant, inhibits serotonin and norepinephrine reuptake, used in depression research.

Dipraglurant: a blood-brain permeable mGluR5 NAM, orally active, selective, IC50 = 21 nM, treats PD-LID.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HPCAL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HPCAL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HPCAL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HPCAL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Anti-Nestin/NES Antibody (9E461) is a Mouse antibody targeting Nestin/NES. Anti-Nestin/NES Antibody (9E461) can be used in ELISA, WB, IHC, IF, FCM.

Paroxetine hydrochloride hemihydrate (Paxil) is an effective and selective serotonin reuptake inhibitor (SSRI).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CHGA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CHGA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CHGA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CHGA in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AchE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AchE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AchE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AchE in the samples is then determined by comparing the OD of the samples to the standard curve.

AMPA Receptor Modulator-5 (Example 217) serves as an AMPA receptor modulator, utilized in neurological disease research [1].

Applications The meta isomer of Fluoromethcathinone (F591580), a new substance of abuse. References Osorio-Olivares, M., et al.: Bioorg. Med. Chem., 12, 4055 (2004), Rosner, P., et al.: Forensic Sci. Int., 148, 143 (2005),

BACE1-IN-5 inhibits BACE1 (IC50: 9.1 nM) & Aβ production (IC50: 0.82 nM), enhances hERG inhibition & P-gp efflux.