Détection de l'ADN

The kit is for use in determining the presence of host cell DNA impurities (to 1 part per billion) in products manufactured by recombinant expression in E. coli. It contains reagents for DNA extraction as well as calibrated DNA standards and primers for DNA amplification in a microplate format, but it does not contain PCR master mix. This kit allows you to test samples with very high concentrations of drug substance without the need for excessive sample dilutions, which effectively lowers the LLOQ of the assay when compared to similar methods.

Used to digest samples containing high level of proteins (~10 mg/ml), which can interfere when determining DNA levels.

This kit is designed for sensitive and specific quantification of residual host cell DNA present in upstream/downstream process samples or drug products manufactured by recombinant expression or viral vectors in human cell lines.

For use in spike and recovery experiments and as standards. Concentration of  ~10 µg/ml

Used to measure residual CHO host cell DNA, this kit uses a 2 ml microfuge tube format and includes our proprietary extraction procedure, which does not use iodine or glycogen. The superior co-precipitating reagents do not interfere with qPCR and ensure that pellets do not become dislodged during extraction. This eliminates the need to centrifiuge samples or repeat a run. Cell line-specific, CHO DNA calibrators are included in the kit. Upon completion of extraction, samples are reacted with PicoGreen® solution. Time to completion is under 2 hours.Each kit supplies enough reagent for the analysis of 50 samples in duplicate.

This kit is designed for sensitive and specific quantification of residual host cell DNA present in upstream/downstream process samples or drug products manufactured by recombinant expression in CHO cell lines.

TE buffer used in DNA analysis and molecular biology procedures.

This kit is designed for sensitive and specific quantification of residual host cell DNA present in upstream/downstream process samples or drug products manufactured by recombinant expression in E. coli. The D1415 kit includes all the necessary reagents and consumables for quantitative PCR from extracted DNA samples obtained by your extraction method of choice. Cygnus offers DNA Extraction Kits (D100T and D100W) developed specifically for efficient and reproducible host cell DNA extraction. The qPCR reagents included in the D1415 kit feature a state-of-the-art master mix containing CleanAmp® dNTPs and Hot Start Taq DNA Polymerase to prevent non-specific priming during PCR setup, and an E. coli-specific primer/probe mix containing FAM-labeled probes that are quenched with BHQ-1™ until PCR extension. The kit includes E. coli DNA concentrate and TE buffer for setting up standard curves and diluting your sample if necessary. This kit is compatible with a variety of qPCR instruments, enabling analysis using your software of choice.

This kit is designed for sensitive and specific quantification of residual host cell DNA present in upstream/downstream process samples or drug products manufactured by recombinant expression in CHO cell lines.

This method utilizes a 2 ml microfuge tube format to measure residual E. coli host cell DNA. The kit includes our proprietary extraction procedure, which isolates residual DNA without the use of iodine or glycogen.The superior co-precipitating reagents do not interfere with qPCR and ensure that pellets do not become dislodged during the extraction procedure. This eliminates the need to centrifuge samples again as well as the need to retest or repeat a run. Cell line-specific E. coli DNA calibrators are included, and upon completion of extraction, samples are reacted with PicoGreen® solution. Time to completion is under 2 hours. Each kit supplies enough reagent for the analysis of 50 samples in duplicate.

For use in spike and recovery experiments and as standards.

This high-throughput method measures residual NS/0 host cell DNA with our proprietary extraction procedure, which does not use iodine or glycogen. Cell line-specific NS/0 DNA calibrators are included, and upon completion of the extraction, the samples are reacted with PicoGreen® solution. Time to completion is under 2 hours for multiple samples and multiple deep-well plates. Each kit supplies enough reagents for the analysis of 96 samples.

This kit is designed for sensitive and specific quantification of residual host cell DNA present in upstream/downstream process samples or drug products manufactured by recombinant expression in CHO cell lines.

This high-throughput method is designed to measure residual human host cell DNA. The kit includes our proprietary extraction procedure for isolating residual DNA without the use of iodine or glycogen. The time to completion of analysis is under 2 hours for multiple samples. Each kit supplies enough reagents for the analysis of 96 samples.

The kit is for use in determining the presence of host cell DNA impurities (to 1 part per billion) in products manufactured by recombinant expression in E. coli. It contains reagents for DNA extraction as well as calibrated DNA standards and primers for DNA amplification in a microtube format, but it does not contain PCR master mix. This kit allows you to test samples with very high concentrations of drug substance without the need for excessive sample dilutions, which effectively lowers the LLOQ of the assay when compared to similar methods.

For use in spike and recovery experiments and as standards. Concentration of  ~10µg/mL

Expression of therapeutic proteins in human cells is a cost-effective method for producing commercial quantities of a drug substance. However, the manufacturing and purification process of these products leaves the potential for DNA impurities from the host cells. This kit utilizes 2 ml microfuge tubes and includes our proprietary extraction procedure to isolate residual host cell DNA without the use of iodine or glycogen. Each kit supplies enough reagent for the analysis of 50 samples in duplicate.

This DNA extraction buffer is for use with wells, 30 ml

PCR assay plate with optical seal, five pack.

100 ng/10 μl x 120 μl