Substrats d'enzymes

Fluorogenic substrate for beta-galactosidase

N-Acetyl-L-serine p-nitroanilide is a fluorescent probe used for the detection of glucose in urine. It has a high purity and is suitable for use as a substrate for many enzymes, including alkaline phosphatase, β-galactosidase, and peroxidase. N-Acetyl-L-serine p-nitroanilide has been shown to be an excellent indicator of bacterial growth in culture media.

4-Nitrophenyl 2-azido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for the enzyme β-galactosidase. It is used in the food industry to test for the presence of milk in foods that are not supposed to contain it. This product also has many other applications, including as a diagnostic tool in clinical laboratories and as an environmental testing agent. The high purity and high quality of this product makes it ideal for use in conjugates with other proteins, such as enzymes or antibodies.

2-Naphthyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate that is used in food testing as well as environmental testing. It has been shown to be a ligand for the enzyme beta-galactosidase. This product is also a chemiluminescent and bioluminescent substrate that can be used in diagnostics and culture media.

4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-alpha-D-galactopyranoside is a chromogenic substrate for alpha-galactosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-alpha-D-galactopyranoside is used in different applications such as the Fabry disease diagnosis, glycolipid metabolism studies

Aldol® 515 beta-D-xylopyranoside is a chromogenic substrate that is used in food testing. It is an alternative to the more common glucose and sucrose, which are not suitable for some types of assays. Aldol® 515 beta-D-xylopyranoside is also used in diagnostics as a ligand for fluorescent antibody staining and chemiluminescence assays. It has been shown to increase the sensitivity of these tests by up to 10 times. The substrate has been tested extensively and is safe for use with a wide range of bacteria including E. coli, S. typhimurium, M. tuberculosis, and C. albicans at concentrations up to 100 mM without any inhibitory effects on growth or viability. END>.

Aldol® 470 L-alanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-alanine aminopeptidase activity. The colorless enzyme substrate is cleaved by L-alanine aminopeptidase, yielding orange-yellow coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 470 L-alanine amide can also be used in enzymatic assays or within live bacterial cultures. Aldol® 470 L-alanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

5-Bromo-4-chloro-3-indolyl acetate (5BCIA) is an enzyme substrate that has been shown to inhibit the activity of a number of enzymes, such as proteases and phosphatases. It has also been shown to inhibit the growth of a number of human pathogens, including Staphylococcus aureus, Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, and Mycobacterium tuberculosis. 5BCIA inhibits bacterial growth by binding to the active site of the enzyme phosphatase and preventing it from hydrolyzing a phosphate group from ATP. This process is necessary for energy metabolism in all living cells. 5BCIA has been shown to be effective against methicillin resistant Staphylococcus aureus (MRSA).

2-(N-Hexadecanoylamino)-4-nitrophenylphosphocholine hydroxide, often abbreviated as HDA-PC, is a chromogenic substrate for acid sphingomyelinase enzyme. HDA-PC is used as a key substrate for measuring the activity of this enzyme, responsible for catalyzing the hydrolysis of sphingomyelin to ceramide. The activity assay for this measuring this enzyme's acitivy is to detect a deficiency (or lack) of the enzyme which is directly related to the Niemann-Pick disease.

5-Bromo-4-chloro-1-methyl-3-indolyl-α-D-glucopyranoside is a fluorescent substrate used for the detection of α-glucuronidase.

3-(4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionamido)propyl b -D -lactoside (FDPBP) is a substrate for alkaline phosphatase. It reacts with the enzyme to produce a blue color. FDPBP is used in diagnostic applications such as food testing and bioluminescence.

Chromogenic substrate for α-glucosidase yielding a salmon-colored precipitate.

Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. This may also be used for in-gel assays

4-(Hydroxymethyl)phenyl-beta-D-triacetylglucuronic acid methyl ester is an enzyme substrate that can be used for bioluminescence, staining, and colorimetric assays. It has been shown to be a good ligand for enzyme substrates such as alkaline phosphatase, glucose oxidase, β-galactosidase, and horseradish peroxidase. This product is a high quality reagent that is suitable for use in diagnostics.

4-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-glucuronide methyl ester is a fluorescent substrate for the beta-glucuronidase enzyme. It can be used to detect the presence of this enzyme in bacteria and other microorganisms by means of fluorometric or colorimetric assays. It has been shown to have an excellent stability in a variety of culture media and food testing applications. This product is available in high purity and quality, with a CAS number of 18472-49-6.

4-Nitrophenyl-beta-L-fucopyranoside is a high-quality pNP enzyme substrate developed for the sensitive detection and quantification of Beta-L-fucosidase activity. The enzymatic hydrolysis of the substrate generates 4-nitrophenol, a yellow chromogenic component that can be conveniently measured spectrophotometrically, allowing for accurate determination of enzyme concentration and kinetics. This substrate finds applications in various research fields, diagnostics, and industrial enzyme production.

Fluorogenic substrate for proglutamyl peptidase 1 yielding a blue fluorescent solution upon cleavage. Used in identification of Enterococcus spp. and group A streptococci (Streptococcus pyogenes).

Please enquire for more information about H-Ala-Phe-Pro-pNA trifluoroacetate including the price, delivery time and more detailed product information at the technical inquiry form on this page

Aldol® 515 L-alanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-alanine aminopeptidase activity. The colorless enzyme substrate is cleaved by L-alanine aminopeptidase produced by microorganisms, grown in liquid or solid media yielding red color. In addition, red fluorescence is generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-alanine amide can be used under aerobic and anaerobic conditions.

chromogenic substrate for beta-glucuronidase

Chemiluminescence phosphatase substrate, broad pH range 6 - 10

A fluorogenic MOSCERDAM® substrate targeting cellulases

2-Nitrophenyl b-D-xylobioside is a chromogenic substrate for the enzyme xylanase.

Chromogenic substrate targeting amylase and maltase

Resorufin pentyl ether is a drug transporter that can be used as an experimental model for studying the effects of chemical pesticides on humans. Resorufin pentyl ether inhibits energy metabolism and inhibits hepatic p450 activity and polymerase chain reaction in hepatocyte-like cells in vitro. It has been shown to inhibit the enzyme activities of rat liver p450s and human cytochrome P450 3A4 (CYP3A4) at physiological levels. Resorufin pentyl ether has also been shown to be toxic in mice, with toxicity studies showing an LD50 of 4.5 mg/kg after oral administration. This drug is not active against bacteria or fungi, but it is able to inhibit protein synthesis in model systems containing mouse monoclonal antibodies or calf thymus DNA.

4-Methylumbelliferyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is a fluorescent marker for nucleic acid molecules. It can be applied to identify the metabolic pathways of bacteria in an ecosystem by using fluorescence microscopy. 4-Methylumbelliferyl 2,3,4,6-tetra-O-acetyl-a-D-glucopyranoside is also used to measure the activity of some enzymes in vitro. This compound has been shown to be effective against spittlebugs and other pests that feed on plant sap. The toxic effect of this compound is due to its ability to inhibit acetate synthesis and disrupt membrane transport systems.

6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin is a fungicide that inhibits the growth of fungi by interfering with their DNA synthesis. It does this by inhibiting the activity of two enzymes: the fungal enzyme oxime and the enzyme cinerea, which is found in many fungi. 6-Chloro-7-hydroxy-4-(trifluoromethyl)coumarin has been shown to be effective against Alternaria and Botrytis cinerea. The compound also has an antifungal effect against other types of fungi, such as Penicillium roqueforti, when used in combination with a solvent such as ether.

Chromogenic substrate for cellobiohydrolase and endoglucanase

Aldol® 518 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase-producing bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 518 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.

Stability Hygroscopic Applications Isocitric acid is the protonated analogue of isocitrate, a substrate of the citric acid cycle. References Zhang, Q., et al.: J. Am. Chem. Soc., 133, 18931 (2011); Crowe, L.M., et al.: Arch. Biochem. Biophy., 242, 240, (1985);

Chlorophenol red beta-D-galactopyranoside sodium salt is a water soluble, colorless salt that is used as a substrate for the reduction of contaminants in soil and water. It can be used to measure the activity of microorganisms in the environment and has been shown to inhibit growth of certain bacteria, such as methylotrophic bacteria. Chlorophenol red beta-D-galactopyranoside sodium salt is also used in biological treatment systems. This product can be mixed with other substances in order to regulate the microbial population and reduce nutrients.

4-Methylumbelliferyl 4-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 4-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis and in screening studies of glycoprotein metabolism disorders.

Methyl 4-methylumbelliferyl hexyl phosphonate is a lipase suicide inhibitor that is a fluorogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases and the fluorescent signal can be detected at Ex 363 nm and Em 465 nm.

4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside is a chemiluminescent substrate that is used in the detection of enzymes. It is also used to measure the activity of beta-glucosidase, alpha-galactosidase, and alkaline phosphatase. The product has been tested for environmental sensitivity and has not shown any effects on bacterial growth. 4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside can be used as a high quality stain for histological sections and immunohistochemical studies. This product has been tested for staining properties with different dyes and has shown no interference with the staining process.

Aldol 458 nonanoate is a high quality, chromogenic and fluorogenic substrate for use in enzymatic, chemiluminescent, and immunochemical reactions. Aldol 458 nonanoate is used as a chromogenic substrate for the detection of glucose oxidase activity in culture media and as a fluorogenic substrate for the detection of glucose oxidase activity in environmental testing. This product has CAS number 2484872-52-6.

4-Methyl-2-nitrophenyl b-D-galactopyranoside is a fluorogenic substrate for β-galactosidase. It has a high purity and is of high quality. 4-Methyl-2-nitrophenyl b-D-galactopyranoside is used as an enzyme substrate in diagnostics, as a chromogenic substrate in staining, and as a conjugate in chemiluminescence. It can be used for the detection of β-galactosidase in culture media and for the detection of β-galactosidase in environmental testing. This chemical also has bioluminescent properties that make it useful for food testing.

This powerful chromogenic substrate is designed specifically for the assay and detection of a-L-fucosidase enzyme activity. With a unique combination of bromo, chloro, and indolyl moieties, it offers enhanced stability and promotes color development that is unparalleled in clarity and intensity. Ideal for biochemistry, molecular biology, and related life sciences applications, this substrate provides unmatched performance and reliability for all your a-L-fucosidase enzyme research needs.

2-Chloro-4-nitrophenyl b-D-lactoside is a chromogenic enzyme substrate used to study the activity of enzymes. It produces a color when hydrolyzed, which makes it a valuable tool in screening for enzyme activity and studying enzyme kinetics.

Aldol 457 Beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase, which is an enzyme that catalyzes the hydrolysis of the terminal galactose residue in D-galactose to form D-galactitol and D-glucose. Aldol 457 Beta-D-galactopyranoside is used as a reagent for the detection of beta-galactosidase activity in biological samples, such as cultured cells or tissues, and has been shown to be a ligand in binding studies with Galectin 1. This product is also used in diagnostic applications such as food testing, culture media, fluorogenic substrates, environmental testing and more.

Also known as BCIP; can be used for visualization of phosphatase labelled antibodies on Western blots. This method quickly became extremely popular in diagnostics and biology research.

Please enquire for more information about 4-Methylumbelliferyl b-D-xylopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

Chromogenic substrate for β-D-glucosidase yielding a magenta precipitate. Differentiation of Salmonella spp. (negative) from other Enterobacteriaceae.

2-Naphthyl beta-D-galactopyranoside is a glycoside that is found in the flowers of the plant Eschscholzia californica. It is used as a localizer for bacterial strains and can be hydrolyzed by enzymes to release naphthalene. 2-Naphthyl beta-D-galactopyranoside has been shown to inhibit the growth of corynebacterium and diazonium salt, which may be due to its ability to bind to DNA and disrupt protein synthesis. The potential use of this molecule is for its insolubility, which prevents it from being absorbed by skin cells.

Stability Hygroscopic Applications Labelled analogue of γ-Butyrobetaine, a synthetic carnitine related compound used as a transporter substrate in the cloning and sequencing of human carnitine transporter 2 (CT2). References Enomoto, A. et al.: J. Biol. Chem., 277, 36262 (2002);

5-bromo-3-indolyl-b-d-glucopyranoside, also known as 5BIG, is an enzyme substrate for beta-glucosidase. It is used in bacterial assays for the identifcation of Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis. After cleavege, it gives an intense blue color.

3-HTC reacts reversibly with thiols and disulfides, and can be used to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates.(λmax: 448 nm in its reduced thiolate form, and a λmax 370-410 nm for the oxidized mixed disulfide).

5-Bromo-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a lapis substrate, a chromogenic substrate that yields a deep blue color after cleavage. It is used in lysosomal storage disorder screening such as for Sanfilippo syndrome.

2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.

4-Nitrophenyl 2-acetamido-3-O-(3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-b-D-glucopyranosyl)-2-deoxy -a -D -galactopyranoside is a Fluorogenic substrate that is used in the detection of beta glucuronidase activity. It can be used for food testing, staining, environmental testing and chromogenic substrate for diagnostic purposes. It has a CAS number of 184840-55-9 and is available in high purity and quality. 4NPPAG is an enzyme substrate that is used to measure the amount of beta glucuronidase activity in culture media by chemiluminescence or bioluminescence.

Fluorogenic substrate targeting esterase and lipase

4-Methylumbelliferyl alpha-L-idopyranosiduronic acid 2-sulfate disodium salt is a chromogenic substrate that is used in the detection of glycosidases. It reacts with enzymes such as alpha-amylase, beta-galactosidase, and beta-glucosidase to produce a fluorescent product. This product can be detected using a fluorometer or spectrophotometer. The CAS number for 4-methylumbelliferyl alpha-L-idopyranosiduronic acid 2-sulfate disodium salt is 1045020-74-3.

DMNPE-caged Luciferin is a photoactivatable probe that can easily penetrate the cell membrane, and by exposure to UV light it undergoes a photochemical decomposition liberating a Luciferin molecule. Once exposed to Luciferase enzyme expressed by the cells, Luciferin emits visible (and measurable) light. Using_x000D_this technique, imaging of luciferase activity was performed both in living cells and in mouse tumor xenograft, by intraperitoneal injection of the caged DMNPE luciferin (Chem. Commun., 2009, 0, 4028-4030).

2-Chloro-4-nitrophenyl β-D-cellotrioside is a chromogenic substrate use in cellulase activity assays. This enzyme, which is responsible to break down cellulose, hydrolyzes 2-chloro-4-nitrophenyl beta-D-cellotrioside and releases 2-chloro-4-nitrophenol than can be quantified spectrophotometrically. The use of 2-chloro-4-nitrophenyl beta-D-cellotrioside as substrate for enzymatic assays allows detecting bacterial cellulose in urine tract infections in the biofilm state.

3,3'-Diaminobenzidine tetrahydrochloride or DAB hydrochloride is the hydrochloride salt of biphenyl tetra-aniline. DAB hydrochloride is a water-soluble formulation of DAB and is used in immunohistochemical staining of nucleic acids and proteins. DAB hydrochloride is used in Alzheimer's disease research, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize causing a colour change. 3,3’-Diaminobenzidine tetrahydrochloride is also available as the tetrahydrochloride hydrate and as the free base.

L-Luciferin is an enzyme substrate that is used in the detection of bioluminescence. It is a fluorogenic substrate that can be used to detect the presence of glucose in urine. L-Luciferin is also a chemiluminescent substrate that can be used to detect ATP, which is required for cell metabolism. L-Luciferin has been shown to be stable at pH levels ranging from 2.5 to 9 and can be stored for up to 3 years when stored at 4°C with no light or oxygen exposure. This product has CAS No. 754978-59-1, indicating high purity and quality. L-Luciferin will not stain or react with other compounds, making it highly suitable for use as a conjugate in diagnostic tests or as a ligand in fluorescent staining reactions.

5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a chemiluminescent substrate that provides a significant increase in light emission. It has a CAS number of 59820-92-0 and is supplied in high purity and high quality. This product is used for the detection of various biological reactions, including diagnostics, enzyme substrates, food testing, and more.

4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).

Butyl 4-nitrophenyl hexyl phosphonate is a lipase suicide inhibitor that is a chromogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases, especially in lipases preferring long chain leaving groups.

Chromogenic substrate for α-amylase yielding a yellow solution upon cleavage.

Chromogenic substrate for N-acetylgalactosaminase

Aldol 491 acetate is a food and environmental testing chemical, which can be used for the detection of bacteria. Aldol 491 acetate provides a chromogenic substrate for the detection of enzymes and can be used as a substrate in enzyme-linked immunosorbent assay (ELISA) procedures. Aldol 491 acetate also has chemiluminescent properties and can be used as a fluorogenic substrate.

The 4-Methylumbelliferyl b-D-ribofuranoside is a top-notch fluorogenic substrate for glycosidase activity detection. Upon hydrolysis by enzymes, it generates a strongly fluorescent 4-methylumbelliferone molecule, enabling rapid assay results and real-time monitoring of enzymatic reactions. This product is suitable for many biological research applications, such as in vitro analysis, diagnostic testing, and drug discovery. With its high sensitivity and specificity, it delivers consistent and accurate results, making it an essential tool for scientists and researchers.

3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.

As an alternative to commonly used colorimetric and fluorescent substrates, 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane, abbreviated AMPPD, is a chemiluminescent substrate for alkaline phosphatase (ALP). It allows detection of alkaline phosphatase at a high sensitivity. Literature reports that the co-presence of 0.1% bovine serum albumin (BSA) leads to a signal increase of 10-folds under selected conditions.

5-Fluorescein isothiocyanate isomer I (5FITC) is a fluorescent probe that has been used to study enzyme activities in human serum. 5FITC binds to the active site of many enzymes, including dopamine β-hydroxylase, and can be detected by measuring the fluorescence intensity. The optimum concentration for this enzyme form is 10 nM for dopamine β-hydroxylase and 20 nM for brain functions. Mouse monoclonal antibodies against 5FITC have also been developed to detect the presence of toll-like receptor 4 (TLR4) on macrophages in vivo. 5FITC has also been used as a fluorescence probe for human immunoglobulin G (IgG) and erythrocytes in blood samples. Colloidal gold conjugates with 5FITC have been developed to measure the disulfide bonds in proteins such as human serum albumin.

5-Bromo-6-chloro-3-indoxyl phosphate, disodium salt monohydrate is a substrate used to visualize phosphatase activity via showing magenta colored end product when used with Nitroblue Tetrazolium.

4-Nitrophenyl beta-D-cellotrioside is a chromogenic substrate for cellulase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-cellotrioside is used in different applications such as the Biomass conversion research, paper industry applications

A fluorogenic MOSCERDAM® substrate targeting alpha-arabinofuranosidase

Chromogenic substrate for beta-glucanase

A substrate for beta-glucuronidase.

Living cells of Escherichia coli can be reliably detected with media that contain a chromogenic or fluorogenic substrate for beta-glucuronidase (also termed β-D-glucuronidase), an enzyme that occurs almost exclusively in E. coli. RUG®, Resorufin-beta-D-glucuronic acid methyl ester is a highly sensitive chromogenic and fluorogenic indicator for E. coli. After removal of the methyl ester group by unspecific hydrolysis, orange colored product is cleaved by beta-glucuronidase in liquid media or on agar plates and yields an intense pink color. Red fluorescence is obtained upon excitation at 560-570 nm.

Chromogenic substrate for ?-glucanase

Naphthol AS-BI N-acetyl-beta-D-glucosaminide is a fluorogenic substrate for the pararosaniline-glyoxalase (PG) reaction. This substrate is used to study enzymatic activity in two human isoenzymes of PG, PG I and PG II. The conversion of this substrate by PG I or PG II results in a color change from yellow to red. Naphthol AS-BI N-acetyl-beta-D-glucosaminide may be used as an experimental tool to measure the activity of these enzymes and to study the effect of inhibitors on their activity. It can also be used as a marker for studying enzyme transfer between cells.

Monohydrate; beta-glucuronidase substrate; yellow/pink color Beta-glucuronidase activity can be measured in a colorimetric assay with phenolphthalein beta-D-glucuronide as substrate. Starting assay conditions can include 30 µg of enzyme-containing material, 0.6 mM phenolphthalein beta-D-glucuronide in 100 mM sodium phosphate buffer in a total volume of 100 µL and monitoring absorbance changes at 540 nm. Temperature incubation can be used to promote the enzymatic activity (Galindo, 2012).

2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used to detect and measure the activity of glycosidase enzymes. Glycosidases are enzymes that hydrolyze glycosidic bonds in carbohydrates, and are produced by many organisms. The substrate is cleaved by glycosidase to release a chromophore, which can be quantified via spectrophotometry analysis. 2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the detection of NAGase activity in biological samples, such as serum, urine, and cell lysates. It is also used for the identification and characterization of glycosidases in microorganisms and the study of glycosylation-related processes in cells and tissues.

5-Bromo-6-chloro-3-indolyl sulfate potassium salt is a fluorogenic substrate that can be used for food testing. It is a conjugate of 5-bromo-6-chloroindolylsulfate and potassium, which is dissolved in buffer solution. This product can also be used as a chromogenic substrate in environmental testing, an enzyme substrate in diagnostics, and a ligand for bioluminescence. It has high purity and quality, and can be used for chemiluminescence or bioluminescence. The CAS number for this product is 6581-24-4.