Substrats d'enzymes

Aldol® 495 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding orange color and green fluorescence. Aldol® 495 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 495 phosphate can be used under aerobic and anaerobic conditions.

Coelenterazine and its derivatives spontaneously complex with aequorin to develop a Ca2+-dependent fluorescent signal.

Substrate for beta-N-acetylglucosaminidase

4-Nitrophenyl 2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that contains a reactive group of 2,3,4-tri-O-levulinoyl. This compound can be used to measure the activity of enzymes such as beta-glucosidase and alpha-galactosidase. 4NP2OMP is an excellent ligand for metal ions and has been shown to bind to copper in the presence of ATP. 4NP2OMP can also be used as a diagnostic agent for measuring the activity of bacterial beta-glucosidase and alpha galactosidase.

6-Chloro-4-trifluoromethylumbelliferyl b-D-galactopyranoside is a chromogenic substrate that can be used in enzyme assays to measure the activity of beta-glucuronidase. This compound is sold as a conjugate with an enzyme, which will not interfere with the assay. The compound has been tested to have high purity and quality.

Applications Fatty acyl esters of 4-methylumbelliferone for use as fluorogenic substrates to continuously follow the hydrolytic activity of carboxylesterases, such as acid and alkaline lipases. References Jacks, T.J., et al.: Anal. Biochem., 21, 279 (1967), Baillargeon, M.W., et al.: Lipids, 26, 831 (1991),

DMNPE-caged form of luciferin

Substrate for a-L-arabinopyranosidase

5-Bromo-4-chloro-3-indoxyl-1,3-diacetate (BCID) is a potent and selective inhibitor of the tyrosine kinase enzyme. BCID has been shown to inhibit the growth of various cell cultures in vitro including murine and human cells. BCID has been shown to be an effective inhibitor of TNF-α production in murine models for asthma and allergic rhinitis. The drug has also been shown to reversibly inhibit the activity of the enzyme without affecting its ability to bind substrate or to regenerate active enzyme form inactive enzyme, indicating that it does not act as a suicide inhibitor.

Chromogenic substrate for beta-D-galactosidase yielding a brown precipitate upon cleavage.

6-Bromo-2-naphthyl beta-D-glucuronide is a substrate for beta-glucuronidase, an enzyme involved in detoxification and bacterial metabolism. Upon enzymatic hydrolysis, it releases 6-bromo-2-naphthol, which forms a detectable dye when reacted with diazonium salts. This compound is widely used in microbiology to detecting E. coli in water testing and histochemical staining. 6-Bromo-2-naphthyl beta-D-glucuronide is also been used in cancer research studies. It has been found that some tumors are related to a high activity of beta-glucuronidase, responsible to reactivate the carcinogens and promote tumor progression, developing a drug resistance to chemotherapy.

Luc-Listeria Na is a chemiluminescent substrate that is used as a reporter molecule in enzyme assays. Luc-Listeria Na reacts with the luciferase enzyme, emitting light. The light emission can be detected using a luminometer and the intensity of light is proportional to the amount of substrate present. Luc-Listeria Na has been shown to react with Listeria monocytogenes and other bacteria that are resistant to conventional staining techniques. This product has passed testing for purity and quality, as well as being tested for its ability to cause an adverse effect on the environment when disposed of.

Aldol 470 acetate is a chromogenic substrate for carboxylesterases yielding a yellow precipitate. Furthermore it shows some fluorescence(excitation at 320 nm to 490 nm). In contrast to classical indoxylsubstrates, Aldol substrates work not only under aerobic but also under anaerobic conditions.

Aldol® 470 nonanoate is a chromogenic substrate with a molecular weight of 496.5 g/mol. Aldol® 470 nonanoate has been used to detect the presence of L-amino acids using a fluorogenic assay. This substrate can be used as a ligand in an enzyme-linked immunosorbent assay (ELISA) to measure the concentration of L-amino acids in food and drink samples, as well as in diagnostics for detecting fluoroquinolone resistance in bacteria. Aldol® 470 nonanoate is also used in the detection of bioluminescence and chemiluminescence reactions. CAS No.: 2484872-50-4

5-Bromo-4-chloro-3-indoxyl sulfate p-toluidine salt is a staining agent used for the detection of glucose in urine and tissues. It is also an enzyme substrate that can be used to detect the presence of lipases, amylases, esterases, phosphatases, and proteases. This product has a CAS number of 6581-23-3 and comes in high quality.