Substrats chromogènes

Aldol® 470 L-alanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-alanine aminopeptidase activity. The colorless enzyme substrate is cleaved by L-alanine aminopeptidase, yielding orange-yellow coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 470 L-alanine amide can also be used in enzymatic assays or within live bacterial cultures. Aldol® 470 L-alanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

4-Nitrophenyl 6'-deoxy-6'-(2-pyridylamino)-a-D-penta-(1-4)-glucopyranoside is a fluorescent substrate for the enzyme beta glucosidase. It is a chemiluminescent reagent that can be used to measure beta glucosidase activity. The substrate is an excellent high quality chemical and has been tested for environmental safety, heavy metal content and staining. 4-Nitrophenyl 6'-deoxy-6'-(2-pyridylamino)-a-D-penta-(1-4)-glucopyranoside is a chromogenic substrate that can be used in diagnostic testing, such as bioluminescence or fluorogenic assay. This product can also be used as a culture media additive or food testing agent.

6-Chloro-3-indolyl b-D-glucuronide sodium salt is a novel chemical entity that disrupts the function of the hsp90. It is an analog of 6-chloro-3-indolyl beta-D-galactopyranoside and is used for coating magnetic particles to be used in devices for detection and amplification of nucleic acid sequences. The compound has been shown to inhibit the interaction of hsp90 with its client proteins, which may lead to decreased levels of protein expression and cell growth. In addition, it has been shown to bind to aluminium surfaces in order to prevent their corrosion, as well as profiling and synchronization assays.

Chromogenic substrate targeting amylase

6-Chloro-3-indoxyl nonanoate is a fluorescent substrate that is used in the detection of beta-galactosidase activity. It has been used to detect the enzyme levels in various culture media and as a high quality, food testing, and environmental testing. The product is also used as a ligand for enzyme inhibition studies. 6-Chloro-3-indoxyl nonanoate has shown to be an excellent fluoroquinolone substrate for chemiluminescence assays. This product is CAS No. 133950-72-8 and is of high purity and high quality.

Chromogenic substrate for ?-maltosidase

S-(4-Nitrophenyl)mercapturic acid (S-NPMA) is the major metabolite of 4-nitrophenol, which is a precursor to dyes and pharmaceuticals. S-NPMA has been detected in urine samples from humans, rats, and mice. The main metabolic pathway for S-NPMA is an oxidative conversion by cytochrome P450 enzymes. S-NPMA has been found to be a useful biomarker for the presence of polycyclic aromatic hydrocarbons in wastewater and human urine. The levels of this compound have also been shown to increase with age in humans.

Enzyme substrate for alpha-glucosidase. Previously under code EN06601.

Chromogenic substrate for β-D-Cellobiosidase yielding a blue precipitate.

Chromogenic beta-maltosidase substrate

Methyl 4-nitrophenyl hexyl phosphonate is a lipase suicide inhibitor that is a chromogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases.

4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a chromogenic substrate that is used to test for the presence of an enzyme. It reacts with the enzyme to produce a light signal that can be detected by a spectrophotometer. The CAS number for this compound is 832476-41-8. This compound has been tested and found to be of high quality and purity. 4NP3OBG is also suitable for use in culture media, food testing, and as a fluorogenic substrate.

Chromogenic substrate for carboxylesterase yielding a blue precipitate upon cleavage. It has been proposed for the rapid detection of Branhamella catarrhalis in a strip test because unlike most other members of the family Neisseriaceae, Branhamella catarrhalis produces a butyrateesterase.

Chromogenic substrate for N-acetylglucosaminidase

Chromogenic substrate for ?-glucuronidase

5-Bromo-3-indoxyl-beta-D-galactopyranoside (5BIG) is a fluorogenic substrate that has been shown to be an effective enzyme substrate. It is used in the chemiluminescence, bioluminescence, and fluorescence detection of enzymes. 5BIG is also a chromogenic substrate for nucleic acid staining and can be used in food testing as well as diagnostics. 5BIG is highly pure and high quality with a CAS number of 1301706-59-1.

Aldol® 495 inositol-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, e.g. PE-Pellets or cellulose acetate filter. Aldol® 495 inositol-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside is a chromogenic substrate for chitinase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl N,N'-diacetyl-beta-D-chitobioside is used in different applications such as the Fungal infection detection, chitin degradation assays

Chromogenic substrate for β-D-galactosidase yielding a precipitate. Mostly used in histochemistry.

6-Fluoro-3-indolyl b-D-galactopyranoside (6-FI-gal) is a chromogenic enzyme substrate often used in microbiology to detect the expression of beta-galactosidase in bacterial colonies. When hydrolyzed by beta-galactosidase, 6-FI-gal produces a blue-green precipitate, allowing for easy identification of colonies that express the enzyme.

Jalapeno green-Gal (Jalapeno green-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a Jalapeno green colored precipitate. Jalapeno green-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach.

Chromogenic β-mannosidase substrate yielding a yellow solution upon cleavage.

5-Bromo-6-chloro-3-indolyl acetate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in bacterial esterase detection (e.g. Staphylococcus spp.).

2,4-Dinitrophenyl b-D-cellobioside is a chemiluminescent substrate that is used to detect the activity of enzymes and other proteins. It can be conjugated with an antibody or other ligand for use in diagnostic purposes. 2,4-DNPBC is a pure compound with a high quality that does not contain any other impurities. Its CAS number is 157956-96-2.

4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-thiogalactopyranoside (NPG) is a fluorogenic substrate that can be used in the detection of beta-galactosidase activity. It reacts with 4-nitrophenol to form an orange product at pH 6.0. The fluorescence intensity increases with increasing concentrations of the substrate and enzyme. The reaction is inhibited by borax, which competes for the enzyme active site. This product has been used as a chromogenic substrate for beta-galactosidase and as an enzyme substrate for beta-galactosidase in diagnostic tests for galactosemia and other carbohydrate metabolism disorders. NPG has also been used in chemiluminescent assays to detect beta-galactosidase activity in culture media and as a bioluminescence substrate in the detection of bacterial

6-Chloro-1H-indol-3-yl b-D-glucuronide is a chemiluminescence substrate that can be used in immunoassays and other biochemical analyses. 6CIG is a highly purified, high quality product with a CAS No. 138182-19-1. This product has been tested for bacterial contamination and found to be free of detectable levels of mycoplasma, yeast, mold, or other microbial agents. It has also been tested for its ability to support the growth of various types of cells and found to be suitable for cell culture media. This chemical has been shown to produce an intense blue chemiluminescent reaction when reacted with horseradish peroxidase enzyme and hydrogen peroxide.

A substrate for alkaline phosphatase yielding a blue insoluble product upon cleavage. Colour can be further enhanced when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. The micronized structure enhances solubility.

Please enquire for more information about 5-Nitro-3-indoxyl-β-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

4-Nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside is a chromogenic substrate for alpha-L-arabinofuranosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside is used in different applications such as the Plant cell wall degradation assays, bacterial enzyme profiling

Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.

Chromogenic β-glucuronidase substrate yielding a yellow solution upon cleavage. Used to detect E. coli contamination in food and drinking water due to their β-glucuronidase activity.

2-Nitrophenyl 6-O-trityl-b-D-galactopyranoside is a chromogenic enzyme substrate used in assays to detect galactosidase activity. It is a derivative of galactose that can be hydrolyzed by galactosidase to release the yellow-colored 2-nitrophenyl group, which can be detected spectrophotometrically. The trityl group helps to stabilize the molecule and prevent undesired hydrolysis during storage. This substrate is commonly used in the study of carbohydrate metabolism and in the detection of galactosidase activity in microorganisms.

b-D-glucosidase substrate for screening of enterococci and C.perferingens

Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases.

4-Nitrophenyl 2,4,6-tri-O-acetyl-3-O-(2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)-β-D-glucopyranoside is an advanced bifunctional chromogenic pNP substrate specifically tailored for the simultaneous detection and quantification of multiple glycosidase enzymes. This dual-action substrate undergoes cleavage by two separate enzymatic reactions, which increases the range and sensitivity of detection. The resulting 4-Nitrophenol release can be easily measured spectrophotometrically, providing rapid and reliable enzyme analysis for advanced enzymology studies, enabling new discoveries in biochemistry and biotechnology.

4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(β-D-galactopyranosyl)-α-D-glucopyranoside is a high-quality chromogenic pNP enzyme substrate specifically designed to enable effective and accurate detection of enzyme activity. By utilizing this advanced substrate, researchers and professionals can perform a range of applications, including activity assays, enzyme inhibition studies, and substrate specificity profiling. This substrate's unique properties result in exceptional sensitivity and throughput, ensuring effective results for a wide variety of enzyme assays.

Chromogenic substrate for carboxylesterase and lipase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a diazonium salt, the corresponding azo-dye is formed. Naphthols can also be detected by fluorescence analysis. Used in an colorimetric assay for the extracellular lipase of Pseudomonas fluorescens B52.

2-O-(4-Nitrophenyl)-a-D-N-glycolylneuraminic acid is commonly used to measure the activity of sialidase enzymes, specifically those that cleave glycoconjugates containing 2-O-(4-nitrophenyl)-alpha-D-N-glycolylneuraminic acid. The resulting product can be detected by measuring the absorbance at a specific wavelength using a spectrophotometer. This substrate offers a high degree of specificity for glycolylneuraminic acid-containing substrates and is useful for both research and diagnostic purposes.

4-Nitrophenyl 4,6-benzylidene-alpha-D-glucopyranoside is a chromogenic substrate for alpha-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 4,6-benzylidene-alpha-D-glucopyranoside is used in different applications such as the Pompe disease diagnosis, glycogen metabolism research

3-Indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a fluorogenic substrate that reacts with the enzyme β-galactosidase to produce a blue product. This product can be used to diagnose the presence of β-galactosidase in culture media, as well as in food and environmental testing. 3IAG is also a chromogenic substrate that can be used for detection of β-galactosidase activity.

6-Chloro-3-indoxyl choline phosphate is a chromogenic substrate for phosphatidylcholine-specific phospholipase C (PC-PLC) for the detection of mibrobial metabolites. The use of this specific substrate permits monitoring the activity of PC-PLC. This an important enzyme for the regulation of steady-state levels of phosphatidylcholine (PC) and diacylglycerol (DAG). Abnormally high levels of PC-PLC activity in various tissues have been linked to many disease progressions.

Chromogenic substrate for beta-D-galactosidase yielding a lapis colored precipitate. 5-Bromo-3-indoxyl-beta-D-galactopyranoside can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside as an alternative to 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), producing a darker blue color for detection of beta-galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants (white) from non-recombinants (blue). 5-Bromo-3-indoxyl-beta-D-galactopyranoside is also used in histochemistry to demonstrate beta-galactosidase activity in tissues.

Aldol® 458 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by phosphatases secreted by bacteria grown in liquid media, yielding yellow color and green fluorescence. Aldol® 458 phosphate can also be used in assays involving phosphatase-coupled antibodies in immunoassay applications (ELISA). Aldol® 458 phosphate can be used under aerobic and anaerobic conditions.

4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate is a chromogenic substrate that can be used to detect the presence of alkaline phosphatase. It is also used in food testing and diagnostics. 4NPPMD is a substrate for the enzyme alkaline phosphatase, which has been shown to play a role in the inflammatory response. The production of 4NPPMD by bacteria in the environment can be detected with chemiluminescence or bioluminescence. This product has been shown to react with both horseradish peroxidase and alkaline phosphatase, as well as other enzymes that are involved in metabolic pathways. This substrate also has high purity and fluorescence properties.

Please enquire for more information about 2-Nitrophenyl-beta-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

Crimson-Gal (Crimson-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a crimson colored precipitate. Crimson-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.

Chromogenic substrate for β-D-glucosidase. Yields a red precipitate upon cleavage.

5-Bromo-6-chloro-3-indolyl b-D-glucuronide (5BCIG) is an antibiotic that inhibits bacterial growth by binding to the DNA gyrase and topoisomerase IV, which are enzymes that maintain the integrity of bacterial DNA. 5BCIG is detectable in tissues, and quantified in blood. It encodes a protein to the cell cytoplasm. The sensitivity of 5BCIG against clostridium perfringens was found to be 10 times higher than that of penicillin G or erythromycin. 5BCIG has optimal activity against clostridium perfringens when expressed functionally as a polypeptide.

Substrate for the detection of beta-D-fucosidase

Chromogenic substrate for cellulases; red color