Substrats chromogènes

4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2,3)-O-b-D-galactopyranosyl-(1,3)-2-acetamido-2-deoxy-a-D-galactopyran oxide is a fluorogenic substrate that is used in the detection of glycosidases. It has been shown to be an excellent ligand for the enzyme beta galactosidase, which cleaves the glycan substrate 4NPOBGPA into 4NP and O-(N acetyl) neuraminic acid. 4NPOBGPA can be used as a chromogenic substrate for detecting beta galactosidase activity and can also be conjugated to other fluorescent molecules. This product is high purity and high quality.

Substrate for a-N-acetyl-galactosaminidase

Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Chromogenic substrate for β-D-glucuronidase (GUS) yielding a magenta precipitate. It is used fordetection of GUS+ bacterial colonies as analternative to 5-bromo-4-chloro-3-indoxyl-β-D-glucuronide (X-GlcA).

5-Bromo-4-chloro-3-indoxyl-alpha-L-arabinopyranoside is a fluorogenic substrate that can be used in enzyme and chromogenic assays. It is a high purity, stable product with a CAS number of 53673-61-7. This product has been shown to have fluorescence properties under acidic conditions, as well as chemiluminescence properties when exposed to an oxidizing agent. This compound can be used for the detection of bacterial activity in culture media and for the detection of pathogens in environmental samples. The conjugate can also be used for food testing.

Aldol 495 nonanoate is a bioluminescent substrate that can be used to measure the activity of Aldolase, which is an enzyme that catalyzes the conversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate to D-glyceraldehyde. This substrate is used in various diagnostic assays, including food testing and staining. Aldol 495 nonanoate was developed by Cymit Quimica Pharmaceuticals and has been patented by EP 2427431 and US 8940909.

Reducing co-substrate for heme peroxidases; test reagent in microbiology

4-Aminoantipyrine has been used as an analgesic and anti-inflammatory agent. It can also target peroxidase enzymes to produce a red color

Aldol® 495 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding orange color as well as green fluorescence if a fluorescence enhancer or a suitable matrix are present. Aldol® 495 beta-D-galactopyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar plates. Aldol® 495 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

4-Nitrophenyl 2-azido-2-deoxy-a-D-galactopyranoside is a superior chromogenic substrate for the precise determination of alpha-D-galactosidase enzyme activity. This substrate features a unique 2-azido moiety providing enhanced sensitivity and specificity in biochemical assays. Upon enzymatic cleavage, it releases the highly fluorescent compound 4-nitrophenol, which simplifies quantification and tracking of enzyme activity in applications such as drug discovery, genetic studies, and enzyme inhibition assays.

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-4,6-O-benzylidene-b-D-glucopyranoside is a compound that is used in gene expression profiling. It has been shown to be an effective inhibitor of glutamicum and corynebacterium glutamicum. 4NPBG can inhibit the production of acid molecules by blocking the activity of cytochrome P450 enzymes in the cells of these bacteria. The compound also inhibits cellular transport and membrane transport genes in these species. This inhibition prevents the synthesis of nucleic acid molecules such as RNA and DNA.

Chromogenic substrate for β-D-Cellobiosidase yielding a salmon-colored precipitate.

N-Methylindoxyl-beta-D-galactopyranoside monohydrate (MG) is a chromogenic probe that interacts with galactose residues found on glycoproteins, which are found on cell surfaces, leading to activation of cells. MG also binds specifically to the surface of lung cells and can inhibit the development of certain types of infections..

Chromogenic substrate for α-galactosidase yielding a salmon-colored precipitate.

5-Bromo-6-chloro-3-indoxyl butyrate is an enzyme substrate that is used for the detection of bacteria and fungi in environmental testing, food testing, and diagnostics. It is also used as a fluorogenic substrate in bioluminescence and chemiluminescence assays. 5-Bromo-6-chloro-3-indoxyl butyrate binds to proteins on the cell surface, causing an increase in fluorescence when excited by light of a specific wavelength. The conjugate can be detected using a variety of methods, including staining with dyes such as safranin or methylene blue. This product has high purity and high quality.

Ostazin brilliant red H-3B hydroxyethylcellulose is a chromogenic enzyme substrate that produces a bright red color upon reaction with specific enzymes. This substrate is particularly useful for the detection and visualization of enzyme activity in histochemical and cytochemical staining techniques. The vibrant red color produced by the reaction allows for easy observation and analysis of enzyme distribution and localization in cells and tissues. Ostazin brilliant red H-3B hydroxyethylcellulose is often used in combination with other substrates to study the presence and activity of multiple enzymes simultaneously, providing valuable insights into the complex interactions and functions of enzymes in biological systems.

2-Bromo-4-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that is used for the detection of β-galactosidase activity in the biochemical reaction. It has been shown to be an excellent ligand for metal ions and can be used as a reagent in the determination of metal ions in biological systems. This product is manufactured with high purity and quality, which ensures its suitability for use in culture media, environmental testing, and diagnostics. 2-Bromo-4-nitrophenyl b-D-galactopyranoside has been tested as a fluorogenic substrate for β-galactosidase. It has also been used as a chromogenic substrate for β-galactosidase and an enzyme substrate for β-galactosidase. This product can be conjugated with other molecules such as biotin to enable rapid analysis of proteins or

Chromogenic substrate targeting beta-L-arabinofuranosidase

6-Chloro-3-indolyl phosphate disodium salt is a substrate for visualising alkaline phosphatase activity. It produces a red colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.

4-Nitrophenyl 4,6-cyclohexylidene-b-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of β-glucosidase activity. It is a high purity product with a ligand that binds specifically to β-glucosidase. 4NPPM is an enzyme substrate that can be used in diagnostics for the detection of β-glucosidase activity and conjugate for chemiluminescence and bioluminescence. This product has been shown to react with glucose in the presence of hydrogen peroxide and luminol, emitting light when excited by ultraviolet light at 365 nm. 4NPPM also reacts with glucose oxidase to produce hydrogen peroxide, which then reacts with luminol, producing light. The CAS number for this compound is 102717-16-8.