CAS: 102110-74-7

Azocasein

Azocasein is a chromogenic substrate, which is derived from casein, with azo dye linkages. It functions by undergoing hydrolysis in the presence of proteolytic enzymes, resulting in the release of soluble azo dye fragments that can be quantified spectrophotometrically. This mode of action allows for the measurement of protease activity by evaluating the intensity of the color change, typically at an absorbance wavelength of around 350-440 nm. Azocasein is widely employed in biochemical assays to determine the activity of various proteases, including those from microbial, plant, and animal sources. Its ease of use and reliability make it a preferred choice in enzyme kinetics studies, enabling researchers to assess enzyme specificity and inhibitor effects. The soluble products of its hydrolysis provide a straightforward way to quantify enzymatic activity, facilitating detailed analyses in both research and industrial applications. As a versatile tool in proteomics, azocasein aids in the understanding of protein digestion and turnover.