CAS 31282-04-9: Igromicina B
Descrizione:
Igromicina B è un antibiotico aminoglicosidico prodotto dal batterio *Micromonospora hygroscopicus*. È principalmente conosciuto per la sua capacità di inibire la sintesi proteica in batteri e alcune cellule eucariotiche, rendendolo uno strumento prezioso nella biologia molecolare per la selezione di organismi geneticamente modificati. Igromicina B è efficace contro un ampio spettro di microrganismi, comprese alcune batteri Gram-positivi e Gram-negativi, così come funghi. Il suo meccanismo d'azione implica il legame con l'RNA ribosomiale, interrompendo il processo di traduzione. Il composto è tipicamente utilizzato in ambienti di laboratorio per la selezione di cellule che sono state trasformate con geni di resistenza alla higromicina. Igromicina B è solubile in acqua e mostra stabilità in varie condizioni di pH, anche se è sensibile alla luce e dovrebbe essere conservato in un ambiente buio. Sono necessarie precauzioni di sicurezza quando si maneggia questo composto, poiché può essere tossico per gli esseri umani e altri organismi ad alte concentrazioni. In generale, Igromicina B è un reagente cruciale nella ingegneria genetica e nella ricerca in biologia cellulare.
Formula:C20H37N3O13
InChI:InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20?/m1/s1
InChI key:InChIKey=GRRNUXAQVGOGFE-HUCHGKBZSA-N
SMILES:O[C@H]1C2(O[C@]3([C@@](O2)([C@@H](O)[C@@H](CO)O[C@H]3O[C@H]4[C@H](O)[C@@H](NC)C[C@@H](N)[C@@H]4O)[H])[H])O[C@]([C@H](CO)N)([C@H](O)[C@@H]1O)[H]
Sinonimi:- (3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-{[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy}-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)octahydro-4H-spiro[1,3-dioxolo[4,5-c]pyran-2,2'-pyran]-3',4',5',7-tetrol (non-preferred name)
- <span class="text-smallcaps">D</smallcap>-Streptamine, O-6-amino-6-deoxy-<smallcap>L</smallcap>-glycero-<smallcap>D</smallcap>-galacto-heptopyranosylidene-(1→2-3)-O-β-<smallcap>D</span>-talopyranosyl-(1→5)-2-deoxy-N<sup>3</sup>-methyl-
- Antihelmycin
- Hygromix 2.4
- Hygromix-8
- Hygromycin B
- Hygromycin B - Solution
- Hygromycin B Streptomyces hygroscopicus
- Hygromycin B solution Streptomyces hygroscopicus
- Hygrovetin
- Hygrovetine
- Vedi altri sinonimi
Ordinare per
18 prodotti.
Hygromycin B
CAS:Hygromycin B binds near the A site of 16S rRNA and induces errors in the code by inhibition of translocation of peptydil-tRNA, however to a lesser extent compared to other aminoglycosides. Hygromycin B is efficient against bacteria, fungi and other eukaryotic cells, but more often used for the selection of eukaryotic cells including yeasts, such as, P. pastoris and S. cerevisiae, and mammalian cell lines, such as, CHO.Formula:C20H37N3O13Purezza:Min. 95%Colore e forma:White PowderPeso molecolare:527.52 g/molHygromycin B
CAS:Hygromycin B disrupts protein synthesis in 70S ribosomes, used as an anthelmintic in swine.Formula:C20H37N3O13Purezza:98% - 99.78%Colore e forma:solidPeso molecolare:527.52Hygromycin B
CAS:Formula:C20H37N3O13Purezza:>80.0%(HPLC)Colore e forma:White to Light yellow to Light orange powder to crystalPeso molecolare:527.52Hygromycin B (HGR), 90%
CAS:Formula:C20H37N3O13Purezza:min. 90%Colore e forma:White to off - white, PowderPeso molecolare:527.52Hygromycin B (HGR Solution), 50mg/ml in Aq. Solution
CAS:Formula:C20H37N3O13Colore e forma:Light yellow to very dark yellow to brown-yellow or light orange to very dark orange, Liquid, Clear to slight hazyPeso molecolare:527.52Hygromycin B (HGR) for tissue culture, 90%
CAS:Formula:C20H37N3O13Purezza:min. 90%Colore e forma:White to off white, PowderPeso molecolare:527.52Hygromycin B Deuterated (d4 major)
CAS:Prodotto controllatoFormula:C20H33D4N3O13Colore e forma:NeatPeso molecolare:531.54Hygromycin B, PBS solution
CAS:Hygromycin B, PBS solutionFormula:C20H37N3O13Purezza:(hplc) min. 90.0% 91.5% (Typical Value in Batch COA)Colore e forma:LiquidPeso molecolare:527.52g/molHygromycin B, powder
CAS:Hygromycin B, powderFormula:C20H37N3O13Purezza:By hplc: 99.4% (Typical Value in Batch COA)Colore e forma: light tan powderPeso molecolare:527.52g/molHygromycin B - min 90%
CAS:Inhibitor of protein synthesis; aminoglycosideFormula:C20H37N3O13Purezza:Min. 90 Area-%Colore e forma:PowderPeso molecolare:527.52 g/molRef: 4Z-H-062002
5mgPrezzo su richiesta10mgPrezzo su richiesta25mgPrezzo su richiesta50mgPrezzo su richiesta100mgPrezzo su richiestaHygromycin B
CAS:Prodotto controllatoApplications Hygromycin B is an aminoglycoside and a selective antibiotic agent for the hph gene. Hygromycin B inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package References Cha, T.S., et al.: World. J. Microbiol. Biotechnol., 28, 1771 (2012); Herrero, L., et al.: Biochem., 52, 171 (2013);Formula:C20H37N3O13Colore e forma:Off-WhitePeso molecolare:527.52Hygromycin B
CAS:An aminoglycoside antibiotic that kills bacteria by inhibiting protein synthesisHygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells that contains the hygromycin resistance gene. This gene is frequently used as a selectable marker in research on plants. It stabilizes the transfer ribonucleic acid (tRNA)-ribosomal acceptor site, thereby inhibiting translocation. In addition to this, it serves as an aminoglycosidic antibiotic which inhibits protein synthesis. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.Formula:C20H37N3O13Colore e forma:Cream to plale brown, PowderPeso molecolare:527.52Hygromycin B, 50 mg/ml in distilled water, sterile-filtered
CAS:Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.Formula:C20H37N3O13Colore e forma:Liquid, Pale yellow to brownPeso molecolare:527.52Hygromycin B
CAS:Formula:C20H37N3O13Purezza:≥ 90.0%Colore e forma:White to beige powderPeso molecolare:527.53
