Rilevamento del DNA

Used to measure residual NS/0 host cell DNA, this kit uses a 2 ml microfuge tube format and includes our proprietary extraction procedure, which does not use iodine or glycogen. The superior co-precipitating reagents do not interfere with qPCR and ensure that pellets do not become dislodged during extraction. This eliminates the need to centrifiuge samples or repeat a run. Cell line-specific, NS/0 DNA calibrators are included in the kit. Upon completion of extraction, samples are reacted with PicoGreen® solution. Time to completion is under 2 hours.Each kit supplies enough reagent for the analysis of 50 samples in duplicate.

The kit is for use in determining the presence of host cell DNA impurities (to 1 part per billion) in products manufactured by recombinant expression in CHO cell lines. It contains reagents for DNA extraction as well as calibrated DNA standards and primers for DNA amplification in a microtube format, but it does not contain PCR master mix. This kit allows you to test samples with very high concentrations of drug substance without the need for excessive sample dilutions, which effectively lowers the LLOQ of the assay when compared to similar methods.

Used for spike and recovery experiments and as standards,10 µg/ml

This high-throughput method measures residual E. coli host cell DNA. It uses our proprietary extraction procedure, which isolates residual DNA without the use of iodine or glycogen. Cell line-specific E. coli DNA calibrators are included, and upon completion of extraction, samples are reacted with PicoGreen® solution. Time to completion is under 2 hours for multiple samples and multiple deep well plates.Each kit supplies enough reagents for the analysis of 96 samples.

The kit is for use in determining the presence of host cell DNA impurities (to 1 part per billion) in products manufactured by recombinant expression in CHO cell lines. It contains reagents for DNA extraction as well as calibrated DNA standards and primers for DNA amplification in a microplate format, but it does not contain PCR master mix. This kit allows you to test samples with very high concentrations of drug substance without the need for excessive sample dilutions, which effectively lowers the LLOQ of the assay when compared to similar methods.

DNA precipitation buffer for use in DNA analysis, 55 ml

This DNA extraction buffer is for use with tubes, 30 ml

This high-throughput method measures residual CHO host cell DNA with our proprietary extraction procedure, which does not use iodine or glycogen. Cell line-specific CHO DNA calibrators are included, and upon completion of the extraction, the samples are reacted with PicoGreen® solution. Time to completion is under 2 hours for multiple samples and multiple deep-well plates. Each kits upplies enough reagents for the analysis of 96 samples.