10X Tris-Tricine-SDS Buffer for molecular biology
Ref. SR-37852
500ml
32.00€
Product Information
Name:10X Tris-Tricine-SDS Buffer for molecular biology
Brand:Sisco Research Laboratories
Description:The Tris-Tricine-SDS Gel Running Buffer is specifically designed for separating proteins with molecular weights between 1 and 100 kDa, making it particularly effective for resolving proteins smaller than 30 kDa. This approach differs from traditional SDS-PAGE by substituting glycine (pK 9.6) with tricine (pK 8.15), which enhances the stacking and destacking of low molecular weight proteins and improves the resolution of smaller peptides due to the differing pK values. Incorporating urea into the stacking gel allows for the effective separation of two proteins that share the same molecular weight. Additionally, the lower acrylamide concentrations in Tricine gels facilitate the transfer of hydrophobic proteins during Western blotting. Tricine-SDS-PAGE is therefore commonly employed for the separation of lower molecular weight proteins.
Notice:Our products are intended for lab use only. For any other use, please contact us.
Chemical properties
Color/Form:Clear, Colourless, Liquid
Technical inquiry about: 10X Tris-Tricine-SDS Buffer for molecular biology
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