Substratos fluorogénicos

4-Methylumbelliferyl pyrophosphate diester disodium salt is a fluorogenic substrate for pyrophosphatases or diphosphatases. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl pyrophosphate diester disodium saltas a substrate for measuring the pyrophosphatases or diphosphatases activity is used for monitoring and diagnosis of pyrophosphatase deficiency and bone metabolism disorder.

4-Methylumbelliferyl alpha-D-xylopyranoside is a fluorogenic substrate for alpha-xylosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-xylopyranosideas a substrate for measuring the alpha-xylosidase activity is used for oligosaccharidosis monitoring and other lysosomal storage disorder diagnosis.

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4-Methylumbelliferyl-beta-D-cellobioside heptaacetate is a fluorogenic substrate for beta-D-glucosidase and can be used to detect this enzyme in the presence of other glycosidases. It is also used as a chromogenic substrate for beta-galactosidase, alpha-mannosidase, alpha-fucosidase, and alpha-N-acetylgalactosaminidase. 4MMB Cellobiose Haptaacetate is a conjugate that can be used to measure the activity of beta-glucuronidase and beta-xylosidase. This product is suitable for use in diagnostics, ligands, food testing, environmental testing, and culture media.

Hexadecanoylamino-4-methylumbelliferyl b-D-galactopyranoside is a substrate used for the diagnosis of Krabbe disease. Krabbe disease (globoid cell leukodystrophy/ galactosylceramide lipidosis), is a rare and often fatal lysosomal storage disease that results in progressive damage to the nervous system. It is inherited in an autosomal recessive pattern and involves dysfunctional metabolism of sphingolipids (MG44866 b-D-Galactosylsphingosine - Synthetic)

A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.

Fluorescein mono-b-D-galactopyranoside or FMG is a fluorogenic substrate for beta-galactosidase. The fluorophore fluorescein is released after enzymatic cleavage, exhibiting a greenish fluorescent. This substrate is particularly valuable in gene expression studies (e.g., lacZ reporter assays) and as a probe for cell-based assays for real-time monitoring.

FLsharp™ - Phosphate, disodium salt is a fluorogenic water soluble substrate for phosphatase enzymes. After cleavage of the substrate by phosphatase activity an insoluble product is formed that is highly fluorescent in the solid state. Upon UV irradiation this very stable fluorophore emits green light in the 530 nm range for an extended period of time. FLsharp™ - Phosphate is a unique substrate - optimized for the detection of phosphatase activity in solid matrices such as agar media, tissuesections or microscopic preparations. FLsharp™ - Phosphate, disodium salt is used in microbiology as asubstrate for bacterial phosphatases. The substrate is suitable to detect the specific phosphatase activity of S. aureus or C. perfringensin food samples or clinical samples. Colonies growing on plating media supplemented with FLsharp™ emit green light upon UV light excitation(Excitation max: 365 nm; emission max: 530 nm). The enzyme product formed from FLsharp™ in agar plating media enables high precision for the localization of the relevant colonies on the plate. As a result small bacteria colonies of phosphatase-positive bacteria can be detected already after a short incubation time.  In cytochemical and histochemical imaging FLsharp™ - Phosphate, disodiumsalt can be used as a substrate for Alkaline Phosphatase (AP)-coupled antibodies. The fluorophore that is released from FLsharp™ - Phosphateby phosphatase activity will precipitate. This insoluble fluorophoreen ables a precise localization of the antibody-bound epitope in cells or tissues under a fluorescence microscope or fluorescence imaging system. The green fluorescence of FLsharp™ can be clearly differentiated from autofluorescence or counterstainings such as (blue) DAPI or (red) Alexafluorophores.

A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.

4-Trifluoromethylumbelliferyl b-D-glucuronide potassium salt is a fluorogenic substrate for beta-glucuronidase. Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl b-D-glucuronide potassium salt is commonly used in bacterial detection and for drug metabolism studies as well as lysosomal storage disorder screening.

L-Proline 7-amido-4-methylcoumarin hydrobromide salt is a fluorogenic peptide substrate for proline-specific peptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.

4-Methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraoside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N'',N'''-tetraacetyl-beta-D-chitotetraosideas a substrate for measuring the chitinase activity is used for fungal infection detection and Gaucher disease screening.

Fluorogenic substrate for N-Acetyl-β-D-glucosaminidase yielding a blue fluorescent solution. Used for rapid identification of Salmonella species.

Fluorogenic substrate targeting beta-glucosidase

Fluorogenic substrate for alpha-D-glucosidase. It has been used in studies of Pompe«s disease (Glycogen Storage Disease). Additionally, it is used for differentiation and enumeration of Enterobactersakazakii, based on alpha-glucosidase activity.

Fluorogenic substrate for b-glucuronidase GUS

6,8-Difluoro-4-methylumbelliferyl b-D-glucuronide is a chemiluminescent substrate for the enzyme glucuronidase. The compound has been shown to be stable in the presence of a wide range of pHs and temperatures. It can be used in applications such as diagnostics, culture media and food testing. 6,8-Difluoro-4-methylumbelliferyl b-D-glucuronide has a high purity level and can be used as an indicator for other compounds such as acetaminophen or ibuprofen.

4-Methylumbelliferyl-alpha-L-iduronic acid, cyclohexylammonium salt is a fluorogenic substrate for the detection of alpha-L-iduronidase activity. It has been used to measure the activity of this enzyme in cells and tissues and as an indicator of tissue damage. The product is also used in food testing, diagnostics, and environmental testing.

6,8-Difluoro-4-methylumbelliferyl b-D-cellotetraoside is a chemiluminescent substrate for the detection of esterases. It is also used as an enzyme substrate in diagnostic applications and as a fluorogenic substrate in food testing. 6,8-Difluoro-4-methylumbelliferyl b-D-cellotetraoside has been shown to be an excellent ligand for use in immunoassays and can be conjugated to antibodies or antigens. 6,8-Difluoro-4-methylumbelliferyl b-D-cellotetraoside is chemically stable and has high purity and high quality.

Resorufin β-D-galactopyranoside is a non-fluorescent compound and is orange-yellow in color. It is hydrolyzed by the enzyme β-galactosidase (β-Gal) to yield fluorescent resorufin.