Compostos relacionados com DNA e RNA

The SafeDye 500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments. The 2,500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production.BioLit™ SafeDye emits green fluorescence when bound to dsDNA, ssDNA and RNA. This stain has a fluorescence excitation maxima when bound to nucleic acid at approx. 290-320nm and emits at 490nm. This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel

The FluroBronze 1kb ladder plus are specially prepared as ready-to-load fluorescent laddersand is ideal for determining the size of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments. The 500bp and 3,000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production.The flurobronze ladder contains in addition to the tracking dye, a non-carcinogenic nucleic acid stain. The ladder should be loaded onto an agaorse gel (of appropriate concentration) containing no fluorescent dye. The stain contained in the ladder replaces ethidium bromide, thus eliminating its addition to the gel.

These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis.The concentration of each band is kept uniform to give sharp bands. The ladder consists of 15 linear double-stranded fragments. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 500bp and 3000bp fragment are present at increased intensity to allow easy identificationSize range : 100 to 10,000 bpContains Bromophenol Blue and Xylene Cyanol as the tracking dyes.Mix concentration :144 ng /µlRecommended loading volume is 7 µl(1µg)

These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 12 linear double-stranded fragments. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 1,000bp fragment is present at increased intensity to allow easy identification. Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes. Size range : 200 to 4000 bpMix concentration : 108 ng /µlRecommended loading volume is 9 µl (1µg)

The FluroBronze 100bp ladder is specially prepared as ready-to-load fluorescent ladders and are ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs. The ladder consists of 11 linear double-stranded fragments .The 500bp fragment is present at increased intensity to allow easy identification. The flurobronze ladder contains in addition to the tracking dye, a non-carcinogenic nucleic acid stain.The new range of DNA ladders are specially prepared as ready-to-load fluorescent ladders. The flurobronze ladder contains in addition to the tracking dye, a non-carcinogenic nucleic acid stain. The ladder should be loaded onto an agaorse gel (of appropriate concentration) containing no fluorescent dye.

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

The SafeDye 100bp ladder are ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs. The ladder consists of 11 linear double-stranded fragments .The 500bp fragment is present at increased intensity to allow easy identification.. All fragments are precisely quantified and mixed during the production. For 5 mcl loading, all fragments except 500bp are 40ng. The 500bp fragment is 100ng.BioLit™ SafeDye emits green fluorescence when bound to dsDNA, ssDNA and RNA. This stain has a fluorescence excitation maxima when bound to nucleic acid at approx. 290-320nm and emits at 490nm. This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel.

FSP Taq Mix Direct for Blood (2X) is a premixed, ready-to-use solution containing FSP Taq DNA Polymerase, dNTP mix, and all other PCR components, except DNA template and primers. FSP Taq Mix Direct for Blood is specific for whole blood amplification. It contributes to fast, specific, sensitive and reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. The FSP Mix (2X) can be used with conventional PCR machines.

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 10 linear double-stranded fragments.A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 2000bp and 5000bp fragment are present at increased intensity to allow easy identificationSize range : 500 to 10,000 bpContains Bromophenol Blue and Xylene Cyanol as the tracking dyes.Mix concentration : 104 ng /µlRecommended loading volume is 9 µl (1 µg)

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that result ion a 3'-dA overhangs PCR product.

These ready -to-Load DNA ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. In this ladder the 500bp and 1200bp fragments are present at increased intensity to allow easy identification. Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes. Size range : 100 to 3000 bp Mix concentration : 136 ng /µl Recommended loading volume is 7 µl (1 µg)

Pfu Mix(2x) is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTP, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intensifier and optimizer. Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.