Compostos relacionados com DNA e RNA

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

FSP Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does, can be used in a variety of PCR. The FSP PCR Buffer, designed for FSP Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FSP Taq DNA Polymerase is 2-fold higher than the one of regular Taq DNA polymerase, which shortens the amplification time by half.

These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 18 linear double-stranded fragments.A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 100bp and 200bp fragment are present at increased intensity to allow easy identification.Size range : 800 to 300 bpContains Bromophenol Blue and Xylene Cyanol as the tracking dyes.Mix concentration :168 ng /µlRecommended loading volume is 6 µl. (1µg)

Description: Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 0.9~1.2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that result ion a 3'-dA overhangs PCR product. The buffer is 10X Roseate Taq Buffer with Mg2+. This buffer has a roseate coloured dye enabling the researcher to load the sample directly after PCR onto an agarose gel for electrophoresis.

FSP Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does, can be used in a variety of PCR. The FSP PCR Buffer, designed for FSP Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FSP Taq DNA Polymerase is 2-fold higher than the one of regular Taq DNA polymerase, which shortens the amplification time by half.

Taq Plus, a mixture of Taq and Pfu polymerase, blends the processivity of Taq with the high fidelity of Pfu. Therefore, this specially formulated Taq plus allow amplification of the higher fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3'-dA overhangs PCR products, which can be used in TA clone.

Taq Plus, a mixture of Taq and Pfu polymerase, blends the processivity of Taq with the high fidelity of Pfu. Therefore, this specially formulated Taq plus allow amplification of higher fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3'-dA overhangs PCR products, which can be used in TA clone.

These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 13 linear double-stranded fragments. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 250bp and 500bp fragments are present at increased intensity to allow easy identification. Contains Bromophenol Blue as the tracking dyes.Size range : 50 to 1000 bpMix concentration :128 ng /µlRecommended loading volume is 8 µl (1µg)

The FluroBronze 500bp ladder is specially prepared as ready-to-load fluorescent ladders ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments. The 2,500bp fragment is present at increased intensity to allow easy identification.. The flurobronze ladder contains in addition to the tracking dye, a non-carcinogenic nucleic acid stain. The ladder should be loaded onto an agaorse gel (of appropriate concentration) containing no fluorescent dye. The stain contained in the ladder replaces ethidium bromide, thus eliminating its addition to the gel.

These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands.A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. Mix Concentration: 100ng/µl Recommended loading volume is 10µl.Size range : 100 to 1500 bp Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes

These Ready-to-Lload ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 10 linear double-stranded fragments. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 2,500bp fragment is present at increased intensity to allow easy identification. Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes.Size range : 500 to 5000 bpMix concentration :92 ng /µlRecommended loading volume is 11 µl (1µg)

Plus Mix (2X) is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTP, Mg2+ and Reaction Buffer a optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer. Taq Plus, a mixture of taq and pfu polymerase, blends the processivity of taq with the high fidelity of pfu. Therefore, this specially formulated Taq plus allow amplification of the higer fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich template. And it is suitable as a direct replacement for ordinary Taq Polymerase inmost applications. In addition, Using Taq plus results in 3'-dA overhangs PCR products, which can be used in TA clone

These Ready-to-Load ladders are designed with precise DNA fragments for accurate quantification by agarose gel electrophoresis. The concentration of each band is kept uniform to give sharp bands. The ladder consists of 8 linear double-stranded fragments. A single or multiple bands in the ladder are made intense to orient the user to the other bands in the ladder. The 250bp fragment is present at increased intensity to allow easy identification. Contains Bromophenol Blue and Xylene Cyanol as the tracking dyes.Size range : 50 to 500 bpMix concentration : 76 ng /µlRecommended loading volume is 13µl (1µg)

2X FSP Taq Mix is a premixed, ready-to-use solution containing FSP Taq DNA Polymerase, dNTP, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. The mix retains all features of FSP Taq DNA Polymerase - possesses high amplification efficiency and fast elongation. Can be used in various kinds of PCR. It has an elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by half. It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity, which results in a 3'-dA overhangs PCR product.

HSP Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. HSP Taq DNA Polymerase can amplify DNA target up to 5 kb. The elongation velocity is 0.9~1.2kb/min. It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity that results in a 3'-dA overhang PCR product. All components of the HSP PCR Buffer are at optimal concentration for efficient amplification. It contributes to highly specific incorporation of primer and template.

Long Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long Taq was shown to amplifiy long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template. Long taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long Taq in your PCR reactions results in 3 ' -dA overhangs PCR products, which can be used in TA clone.