Substratos enzimáticos

Chromogenic substrate for cellobiohydrolase and endoglucanase.

3-Indoxyl phosphate p-toluidine salt is a secretor of blood group A. It is used in the detection of the presence of alkaline phosphatase in serum and other body fluids. This compound has been shown to be an isoform of 3-indoxyl phosphate with a molecular mass of about 4,000 daltons. It is also used as a substrate for gel electrophoresis and filtration experiments. The purified form of this compound has been shown to have an alkaline phosphatase activity that is ˜4 times greater than that found in human serum and intestinal extracts.

4-Nitrophenyl 2-acetamido-3-O-(2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2,6,8,10,12-pentaoxoheptaacetic acid is a chemiluminescent substrate that is used for the detection of peroxidase and other enzymes. The reaction product is a yellow fluorescent product which can be measured using a fluorometer. It is sold as an aqueous solution at 1mg/mL in phosphate buffer at pH 7.4.

4-Methylumbelliferyl-a-D-mannopyranoside-2-phosphoethanolamine is a fluorescent substrate used in enzyme assays. It is an analog of the natural substrate, 4-methylumbelliferyl-alpha-D-mannopyranoside, which is a phosphoethanolamine linked to alpha-D-mannose. This product can be used as a chromogenic or fluorogenic substrate for various enzymes, including beta glucosidase, beta galactosidase, and beta lactonase. 4MUMPE can also be used for bioluminescent studies and has been shown to have high purity and quality.

Naphthol AS-D is a diagnostic chemical for phosphatase enzymes and is used to stain cells, tissues, and body fluids for diagnosis. The chemical reacts with the amino groups of proteins in cells and produces a brown color. Naphthol AS-D is not toxic to living cells in concentrations below 5%. It stains basophilic leukemia cells as well as brain cells. This chemical can be used to diagnose cutaneous lesions, such as psoriasis or melanoma, which are difficult to diagnose by standard methods.  present in these lesions and produce a brown color.

Aldol® 515 palmitate is a chromogenic substrate that is used in food testing, diagnostics, and other applications. It has a high purity and is conjugated to various ligands for specific applications. Aldol® 515 palmitate is an excellent chemiluminescent substrate for the detection of ATP and other nucleotides. It also has high bioluminescence activity for the detection of adenosine-5'-triphosphate (ATP) or luciferin as well as enzyme substrates to measure β-galactosidase or β-glucuronidase activity.

5-Bromo-3-indoxyl-beta-D-galactopyranoside-6-sulfate sodium salt is an enzyme substrate that has been used in diagnostic tests for detecting the presence of alpha galactosidase. 5-Bromo-3-indoxylbDgalactopyranoside 6 sulfate sodium salt is a ligand that has been shown to bind to the receptor on macrophages and neutrophils, which are cells that protect the body from infection. 5Bromo3indoxylbDgalactopyranoside 6 sulfate sodium salt can be used as a chromogenic substrate or fluorogenic substrate for chemiluminescence reactions. It can also be used in culture media for bacterial growth and for food testing.

Coelenterazine e is a synthetic, fluorescent amino acid that can be used to measure calcium ion concentration. It binds to calcium ions and emits light when excited with blue light. Coelenterazine e is an analog of coelenteramide, which is a luminescent protein found in jellyfish. Coelenterazine e has been shown to emit fluorescence when it interacts with calcium ions, while coelenteramide emits bioluminescence instead. The emission of light from coelenterazine e is due to the stabilization of the molecule by calcium ions.

Used for synthesis of chiral carboxylic acids from nitriles

6,8-Difluoro-4-methylumbelliferyl b-D-galactopyranoside, commonly known as DiFMUG, is a fluorogenic substrate to detect beta-galactosidase. It has higher sensitivity than the common MUG (4-methylumbelliferyl beta-D-galactopyranoside) subtrate.

Aldol® 518 acetate is a fluorogenic substrate for the enzyme aldolase. It is used in diagnostic kits for detecting aldolase in blood and other body fluids, and as an indicator of liver function. Aldol® 518 acetate can be used to detect the presence of aldolase in foods and beverages. The compound has also been shown to be an excellent substrate for bioluminescence and chemiluminescence reactions, which are widely used in research laboratories.

A fluorogenic MOSCERDAM® substrate targeting cellulases

Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.

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Luciferin-caprylate is a chemiluminescent substrate that is used in the detection of various substances. It is a high purity compound with CAS No. 583-22-8 and an E number of E711. This product can be used in diagnostic, food testing, and other applications. Luciferin-caprylate has been shown to be an excellent substrate for bioluminescence, fluorescence, and chemiluminescence reactions. It generates light through the oxidation of luciferin by the enzyme luciferase in the presence of oxygen or other oxidizing agents. The light generated by this reaction can be detected using a photomultiplier tube or luminometer, which can then be used to detect substances such as bacteria, protozoa, or fungi.

Chromogenic substrate for ?-glucosidase

6-Bromo-2-naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.

6-Chloro-1H-indol-3-yl b-D-mannopyranoside is an enzyme substrate that is used in the fluorogenic and chromogenic assays for detecting indoleamines. This compound has a conjugate, which is a fluorescent molecule that can be attached to an antibody to target specific proteins or cells. 6-Chloro-1H-indol-3-yl b-D-mannopyranoside is used as a diagnostic agent in the detection of bacterial and fungal infections. It is also used as an indicator of cell growth in culture media.

Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside can be used to inhibit the growth of bacteria, yeast, and fungi. It is a yellow powder that has been shown to cause regression in tumor xenografts in mice. Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside inhibits cellular proliferation by blocking the synthesis of proteins and nucleic acids. This compound also has been shown to have an effect on the environment because it inhibits the growth of phytoplankton. Phenylethyl 2-acetamido-2-deoxy-b-D-glucopyranoside was found to disrupt bacterial gene expression profiles, which may be due to its ability to inhibit protein synthesis at the ribosome level.

2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a synthetic substrate for various enzymes, including the enzymes that catalyze the conversion of D-glucose to glucose 6 phosphate. This compound is used as a growth factor and has been shown to inhibit chronic kidney disease in rats. In addition, it has also been shown to regulate matrix metalloproteinase activity in human urine.

1-Naphthyl beta-D-glucuronide (NPG) is a chromogenic enzyme substrate used to detect the presence of β-glucuronidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is commonly used in clinical assays to detect the presence of the enzyme in urine samples, as elevated levels can indicate liver or urinary tract diseases. It is also utilized in environmental testing, as some bacteria strains can produce the enzyme as a by-product, making NPG a useful tool for detecting these strains in contaminated water or food sources.

6-Chloro-3-indolyl b-D-cellobioside is a chromogenic substrate that produces a salmon-pink color after enzymatic cleavage. It is used for detecting the activity of beta-D-cellobiosidase in enzyme assays.

3-Indoxyl sulfate potassium salt (3ISKP) is a microbial metabolite that has been shown to have an optimum concentration in the range of 0.5-2.0 mM. It is water soluble, and can be found in human serum and human feces. 3ISKP is synthesized from tryptophan by gut bacteria as well as from dietary sources such as soybean protein. 3ISKP has been shown to inhibit mitochondrial membrane potential, which may lead to apoptosis of cells or inhibition of physiological function. 3ISKP also inhibits fatty acid synthesis, which may affect the metabolism of other nutrients. The analytical method for 3ISKP is LC-MS/MS with a reaction solution containing methanol and acetonitrile in a ratio of 1:1 with a pH range of 4-5.

2-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to detect β-galactosidase activity. This specific substrate is one of the most commonly used substrates for detecting β-galactosidase activity in diagnostic assays. It reacts with the enzyme to produce a blue color and has a fluorescence emission maximum at about 480 nm. The product is available in high purity and high quality and it is also an enzyme substrate.

Galβ(1-3)[neu5acα(2-6)]GlcNAc-β-PNP is a glycosylation product of the complex carbohydrate GlcNAc. It is synthesized by the enzyme β-1,3-galactosyltransferase and N-acetylneuraminic acid transferase. This product has been modified using click chemistry to introduce a fluorine atom at the 6 position of Neu5Acα2-6. Galβ(1-3)[neu5acα(2-6)]GlcNAc-β-PNP is available in high purity and custom synthesis.

Chemiluminescent substrate for b-galactosidase

Applications Met-enkephalin analog found in opioid extracts of brain and adrenal chromaffin cells that is an artifact generated by trichloroacetic acid extraction. MUS is used as a fluorimetric substrate for arylsulphatase enzyme. Subsitution of the hydroxyl group of 4-methylumbelliferone (4-methyl-7-hydroxy-coumarin) causes the excitation wavelength of the umbelliferone to shift to shorter values and also brings about a decrease in fluorescence.Fluorescence: max. Abs = 334 mm; max. Em. = 370 mm References Harinath, B., et al.: J. Neurochem., 18, 245 (1971), Marniemi, J., et al.: Int. J. Biochem., 4, 95 (1973), Bojarova, P., et al.: ChemBioChem., 9, 613 (2008),

Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their β-galactosidase activity.

5-Bromo-6-chloro-3-indolyl b-D-cellobiopyranoside is a fluorogenic substrate for the enzyme β-glucuronidase. It is used in diagnostic applications such as food testing, bacterial culture media and environmental testing. The product has CAS number: 123591-77-0.

Chromogenic substrate for N-acetyl-β-D-glucosaminidase yielding a blue precipitate. Contained in certain mycological culture media. Used in histochemistry.

2-Amino-5-pyridyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-galactopyranose is a chromogenic substrate for the enzyme β galactosidase. It is used to detect the presence of β galactosidase in bacterial cultures. The substrate is soluble in water and has a long shelf life. 2APAAG can be used for food testing and environmental testing as it produces a bioluminescent signal when cleaved by β galactosidase.

Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol® substrates can be used under aerobic and anaerobic conditions.

Resorufin b-D-glucuronide sodium salt is used as a fluorogenic substrate for beta-glucuronidase (GUS) to study drug metabolism.

4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is an extracellular nucleic acid molecule that is produced by the bacterium Corynebacterium glutamicum. This compound has been shown to inhibit cancer cells by blocking the transport of glucose through the cell membrane and may be a potential drug target for cancer treatment. 4NPTAPG has also been shown to have diagnostic properties and can identify corynebacterium glutamicum strains. It is a fluorescent molecule that can be used to detect the presence of this bacterium in culture media.

Please enquire for more information about 4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

2-Aminophthalylhydrazido 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate that is used as a diagnostic in biological research. It reacts with enzymes to form fluorescent products, and can be used to detect the presence of bacteria and fungi. This product has a CAS number of 128879-80-1 and can be used for food testing, environmental testing, or bioluminescence. It is an important component in the development of new antibiotics.

[2-Methoxy-4-(2-nitrovinyl)phenyl] beta-D-galactopyranoside is a chromogenic substrate (505 nm) for β-glucosidase.

Disodium salt trihydrate

A MOSCERDAM® substrate targeting the fucosidase enzyme

Fluorogenic substrate for beta-glucosidase yielding a blue fluorescent solution. Used for the detection of beta-glucosidase as an indicator of Enterococci.

5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-mannosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is used in alpha-mannosidosis diagnosis and other lysosomal storage disorder.

4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-galactopyranoside is a fluorescent marker that is used to measure the enzymatic activity of corynebacterium glutamicum. The enzyme catalyzes the conversion of 4-methylumbelliferyl glucopyranoside to 4-methylumbelliferone glucuronide. The production of 4 methylumbelliferone glucuronide can be quantified using fluorescence spectroscopy. This product can be used in research involving chronic inflammatory diseases and cancer.

AquaSpark® Peroxide Probe is a dioxetane-based substrate for the luminescence detection of hydrogen peroxide and other peroxides in biological samples. AquaSpark® Peroxide Probe is the most sensitive probe for H2O2 detection available. It is also an extremely versatile probe for oxidase detection applicable in a wide range of biocompatible buffers. For example it can be used for glucose oxidase detection andfor the quantitation of glucose levels (when coupled to the glucoseoxidase activity in an assay). In addition, AquaSpark® Peroxide Probe is used for the quantification of other biologically relevant compounds(e.g. amino acids) using the corresponding peroxide releasing oxidases.

5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt - micronized is a chemiluminescent substrate used in diagnostic assays. It is an enzyme substrate that is commonly used to detect peroxidase activity and has been shown to be effective in detecting various types of bacteria. This product is a high quality chemical with a purity of at least 98% and has been tested for environmental safety. 5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine salt - micronized can be used as a fluorogenic substrate for the detection of proteases and aminopeptidases.

5-Bromo-4-Chloro-3-Indolyl b-D-Mannopyranoside, also known as X-Man, is an enzyme substrate commonly used for detecting mannosidase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of mannosidases involved in glycoprotein processing and quality control.

Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ coliform culture media. About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products and shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants. The sodium salt has advantages over the cyclohexylammonium salt as being non-electrostatic and possessing a naturally-occurring cation.

A fluorogenic substrate for the detection of lysosomal enzyme N-acetyl-α-D-galactopyranosiaminidase (NAGA), which is driving the pathogenesis of Schindler disease. Also substrate for endo-α-N-acetylgalactosaminidase (O-glycosidase), which cleaves O-linked core 1 and core 3 disaccharides from glycoproteins.

Please enquire for more information about Ac-D-Cha-GR-aminoluciferin including the price, delivery time and more detailed product information at the technical inquiry form on this page

Chromogenic substrate targeting alpha-L-arabinofuranosidase

5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).

Aldol® 495 nonanoate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C9 esterases, yielding development of an orange coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 495 nonanoate can be used in enzyme assays and live bacterial cultures. Aldol® 495 nonanoate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.

AquaSpark® H2S/-SH is a chemiluminescent probe for the detection of sulfides and thiols. The chemiluminescence obtained through sulfide is 3-5 fold higher than chemiluminescence obtained through thiols. AquaSpark® H2S/-SH probe was used in a study identifying antibiotic-resistant bacterial strains by detecting the beta-lactamase-driven hydrolysis of beta-lactam antibiotics, where hydrogen sulfide is a biodegradation metabolite (Popat Gholap et al, 2021).

Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.

4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-2-acetamido-2-deoxybDglucopyranoside is a fluorescent compound that has been used as a substrate in enzyme assays. It has been shown to be an effective ligand for the enzyme beta galactosidase, which hydrolyzes the substrate to produce 4nitrophenol and o-(Nacetyl)a-neuraminic acid. This product is CAS No. 501427-92-5 and can be used as a high purity, high quality reagent for conjugation.

4-Methylumbelliferyl-alpha-L-arabinofuranoside is a nucleic acid molecule that has an average diameter of 10 nm. It is used as a tracer in electron microscopy to detect the presence of nucleic acid molecules in cells. This compound is also used to study the interactions between DNA and RNA molecules by labeling the DNA strand with 4-methylumbelliferyl-alpha-L-arabinofuranoside, which contains an alpha-(2,4,6) arabinofuranosyl group. The recombinant expression of this compound can be carried out in corynebacterium glutamicum, and it has a bulk density that ranges from 1.7 to 2.1 g/cm3 and an average particle diameter ranging from 0.5 to 1 micron. It is not soluble in water or alcohol but is soluble in acetone or chloroform.

Applications A novel fluorigenic substrate for the determination of Biotinidase activity. References Kumugi, S., et al.: Chemistry Letters, 391 (1997)

Fluorogenic substrate for esterase yielding a blue fluorescent solution upon cleavage.

This Clips-O substrate rac-7-(3',4'-Dihydroxybutyloxy)coumarin-4'-phosphate allows the detection of single and dual enzymatic activity (phosphatase and oxidation of 4-amino-3-hydroxybutyloxy spacer either enzymatically or by exposure to sodium periodate) with a single substrate resulting in a fluorescent signal. The main virtues of Clips-O substrates are an increased stability towards non-enzymatic hydrolysis that results in a reduced background and high sensitivity.

Resorufin phosphate disodium is a fluorogenic substrate for phosphatase exhibiting a red color after cleavage that can be measured at ~ 585nm.

5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-galactosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is used in lactose intolerance screening.

2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid, also known as p-nitrophenyl N-acetylneuraminic acid, is a chromogenic substrate specifically designed for the detection and quantification of neuraminidase (sialidase) activity. Upon cleavage by neuraminidase, it releases a yellow-colored product, 4-nitrophenol, which can be easily monitored spectrophotometrically at 405 nm. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of neuraminidase activity in biological samples such as viruses, bacteria, and cell lysates. It is particularly useful for studying the enzymatic properties of neuraminidases from different sources and for the development of novel antiviral and antibacterial agents targeting neuraminidase activity.

2'-(4-Methylumbelliferyl)-a-D-N-glycolylneuraminic acid is a fluorogenic substrate that is used for enzymatic labeling of glycolipids. It can be used as a diagnostic or food testing agent, and also has applications in environmental testing. The CAS number for this product is 1434265-32-3.

4-Nitrophenyl 4,6-ethylidene-?-D-maltoheptaoside is a substrate used in a reference measurement method for ?-amylase activity in the diagnosis of acute pancreatitis, approved by the International Federation of Clinical Chemistry (IFCC). The substrate is hydrolysed by serum ?-amylase into shorter 4-nitrophenylated oligosaccharides and their free counterparts. These reaction products are channeled into an indicator reaction with ?-glucosidase and are degraded into glucose and the chromophore, 4-nitrophenol.

4-Methylumbelliferyl 6-O-(N-acetylneuraminyl)-b-D-glucopyranoside is a fluorescent substrate for the enzyme β-D-galactosidase. It is used in the detection of β-D-galactosidase activity in the diagnosis of various diseases, including celiac disease and AIDS. 4-Methylumbelliferyl 6-O-(N-acetylneuraminyl)-b-D-glucopyranoside can also be used as a chromogenic or fluorogenic substrate for chemiluminescent detection of β-D-galactosidase activity. The conjugate with horseradish peroxidase can be used to detect hydrogen peroxide in water samples, such as drinking water, wastewater, and surface waters. 4MUG has been shown to have high purity and is CAS No. 78761-35-4.

Chromogenic substrate targeting alpha-D-galactosidase

Living cells of Escherichia coli can be reliably detected with media that contain a chromogenic or fluorogenic substrate for beta-glucuronidase (also termed β-D-glucuronidase), an enzyme that occurs almost exclusively in E. coli. RUG®, Resorufin-beta-D-glucuronic acid methyl ester is a highly sensitive chromogenic and fluorogenic indicator for E. coli. After removal of the methyl ester group by unspecific hydrolysis, orange colored product is cleaved by beta-glucuronidase in liquid media or on agar plates and yields an intense pink color. Red fluorescence is obtained upon excitation at 560-570 nm.Formally ER175455.

4-Nitrophenyl 3,4-O-isopropylidene-a-D-galactopyranoside is a chemiluminescent substrate for detecting beta-galactosidase activity in bacteria. This product is a high purity, high quality compound that can be used as a culture media and environmental testing agent. It has been shown to be effective in diagnostics, chromogenic substrate, and enzyme substrate reactions. 4NPPG has been shown to be a fluorogenic substrate for beta-galactosidase assay and can be used as food testing agent with conjugate.

4-Nitrophenyl 2-acetamido-3-O-acetyl-4,6-O-benzylidene-2-deoxy-aDglucopyranoside is a fluorogenic substrate that can be used for the detection of β-glucosidase activity. It is a water soluble substrate with an extinction coefficient of approximately 10,000 M−1 cm−1 at 420 nm. 4NPAABGDG is a chromogenic substrate that can be used to detect β-galactosidase activity. It has an extinction coefficient of approximately 1,700 M−1 cm−1 at 420 nm. The CAS number for 4NPAABGDG is 23262-56-8 and it is sold by Sigma Aldrich under catalog number L4959.

4-Methylumbelliferyl 3,4,6-tri-O-acetyl-b-D-galactopyranoside is a fluorescent compound that can be used as a substrate for the following enzymes: β-galactosidase, α-galactosidase, and β-glucuronidase. This product is used in diagnostics to measure the activity of these enzymes. It can also be used to detect bacteria in culture media and food testing. 4MUF3,4,6TABG is a high quality product that has been shown to have a high purity level by liquid chromatography. CAS number: 61440-03-2

Fluorogenic substrate for ?-galactosidase

A substrate for visualising alkaline phosphatase activity. It produces a purple insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. DMF soluble.

Fluorogenic substrate for aminopeptidase

Histamine (Scombrotoxin) fish poisoning is the most frequent cause of fish poisoning incidents today. Cymit Quimica's BCM®; Histamine-producingbacteria Broth (C-0450) is a growth medium for the detection of histamine-producing bacteria in food and water samples. The medium contains an indicator that turns orange to red due to metabolites released by histidine decarboxylase.

4-Nitrophenyl 6-O-(alpha-D-glucopyranosyl)-beta-D-glucopyranoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 6-O-(alpha-D-glucopyranosyl)-beta-D-glucopyranoside is used in different applications such as the Cellulose metabolism research, biofuel production

2-Chloro-4-nitrophenyl b-D-cellobioside (CNPG) is a chromogenic enzyme substrate that is commonly used to detect and measure the activity of β-glucosidases. CNPG is cleaved by the enzyme into the chromogenic product, which can be detected spectrophotometrically. β-glucosidases are important enzymes in the breakdown of cellulose, and CNPG is a widely used substrate for the detection and quantification of these enzymes in various research studies.

AquaSpark™ α-D-galactoside is a dioxetane-based substrate for the luminescence detection of α-galactosidase activity. It is the first single-compound chemiluminescent substrate commercially available for the detection of α-galactosidase activity. α-galactosidase assays are used in molecular biology to monitor the transcriptional activity of the GLA gene that is correlated with metabolic diseases or gene mutations. In microbiology the α-galactosidase activity is used to detect specific bacteria such as α-galactosidase positive Salmonella strains (Salmonella Enteritidis RKI 05/07992).

6-Chloro-1H-indol-3-yl octanoate is a fluorogenic substrate that can be used to detect enzymes such as phosphatases, lipases, esterases, and proteases. 6-Chloro-1H-indol-3-yl octanoate is a ligand for metal ions such as Cu(II), Ni(II) and Zn(II). This product has high purity and quality. It is an enzyme substrate for many enzymes such as phosphatases, lipases, esterases, and proteases. This product can be used in diagnostics of various substances including food testing, environmental testing and staining. 6CIO is a chromogenic substrate that can be used to detect bioluminescence reactions. 6CIO is CAS No. 159954-35-5.

2-Nitrophenyl 2,3-di-O-acetyl-b-D-xylopyranoside is a chromogenic substrate used to assay the activity of xylosidase enzymes. It is hydrolyzed by xylosidase to yield a yellow-colored product, 2-nitrophenol, which absorbs light at 405 and 410 nm. This substrate is used in research to study the function of xylosidase enzymes and their role in various biological processes. It is also used in diagnostic tests for the detection of xylosidase activity in different microorganisms, including bacteria and fungi.