Substratos enzimáticos

Chromogenic substrate for alkaline phosphatase

4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is a fluorescent substrate for the enzyme beta-glucosidase. It reacts with an acyl group at the C2 position of the glycoside moiety, producing an aldehyde group at the C1 position and a hydroxymethylene group at the C4 position. This reaction is catalyzed by beta-glucosidase, which cleaves the glycosidic linkage in sucrose and other disaccharides. The product can be detected using luminescence methods, such as chemiluminescence or bioluminescence. 4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is used to detect β glucosidase activity in bacteria. This substrate has been shown to be sensitive and specific for β glucosidase activity when combined with fluorescence detection technology

a-Naphthyl phosphate disodium salt hydrate is a highly water-soluble chromogenic substrate used to determine phosphatase activity in research and analytical applications, particularly for staining and differentiating acid and alkaline phosphatases.

2-Nitrophenyl alpha-D-galactopyranoside is a chromogenic substrate used to detect the enzymatic activity of alpha-galactosidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl alpha-D-galactopyranoside is used in Fabry disease screening.

Coelenterazine-v is a coelenterazine analog that emits a red fluorescent light in the presence of calcium (Ca2+). It is used as a reporter molecule for intracellular calcium concentration. Coelenterazine-v has been used in vitro to detect protein-protein interactions and in animal experiments to detect luciferase activity. Other coelenterazine-v analogs have also been developed with different color properties, including blue, green, yellow, orange, and purple. All these analogs, including coelenterazine-v, are used for optical imaging of cells or tissues or for detecting the activity of luciferases.

4-Trifluoromethylumbelliferyl b-D-xylopyranoside (4TUF) is a fluorescent probe that can be used to monitor the activity of protein kinases. The fluorescence intensity of 4TUF changes in response to the phosphorylation state of the target protein. This probe can be used in computational and experimental investigations of protein kinase signalling pathways, as well as for optimizing drug design.

Substrate for determining hydrolytic activity of chitinase

4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.

Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191 is a chemiluminescent ligand that can be used for detecting and quantifying proteins, nucleic acids, proteins, carbohydrates and lipids. Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191 has been shown to be a high quality and high purity reagent with excellent stability in the presence of heat, light or pH changes. The product has been tested for safety in cell cultures and animals, as well as for its effects on the environment. It has also been found to be safe for use in food products. This chemiluminescent ligand is an ideal tool for diagnostic purposes, such as detection of bacteria or viruses in culture media or environmental testing.

6-Chloro-4-methylumbelliferyl b-D glucuronide is a fluorogenic substrate for beta-glucuronidase that exhibits longer absorption/emission maxima (~385/500 nm) than typical 4MU-derived substrates.

4-Nitrophenyl b-D-glucuronide sodium salt is a high-quality chromogenic substrate specifically designed for the colorimetric detection and measurement of β-glucuronidase activity. Upon enzymatic cleavage, it releases the yellow 4-nitrophenol, enabling quantitative analysis via spectroscopy. This compound is conveniently provided as a sodium salt to ensure enhanced solubility, stability, and shelf life. It is an ideal choice for biochemical, research, and diagnostic applications requiring a reliable and efficient b-D-glucuronide substrate.

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Luc-E.coli K is a protease substrate that is used as a reagent for the detection of enzymes in bioluminescence, chromogenic, and fluorogenic substrates. Luc-E.coli K is also used to detect the presence of specific ligands or antigens in diagnostic assays, such as immunoassays, enzyme-linked immunosorbent assay (ELISA), and radioimmunoassay (RIA). Luc-E.coli K is a high purity product with a CAS number.

Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.

2-Nitrophenyl-beta-D-galactopyranoside is used as chromogenic substrate for quantitative studies and enzyme kinetics of beta-galactosidase. It is used in assays for quantification of the activity of beta-galactosidase reporter gene constructs in molecular biology. This test is routinely used in diagnostics for the identification of lactose-fermenting bacteria of the Enterobacteriaceae family.

Fluorogenic substrate targeting plasmin

Thymidine 5′-monophosphate p-nitrophenyl ester sodium is a fluorogenic substrate that is used in food testing and environmental testing. It is an important component of the Ligand Assay, a diagnostic test for the detection of antibodies to HIV. Thymidine 5′-monophosphate p-nitrophenyl ester sodium has been shown to be a good substrate for use in enzyme assays, such as DNA polymerase, DNA ligase, and RNA polymerase. This product is also used in bioluminescence and chemiluminescence reactions.

Substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.

2-Chloro-4-nitrophenyl-beta-D-galactopyranoside is a chromogenic substrate used to test enzyme activities. It is commonly used in molecular biology research for the detection and measurement of beta-galactosidase activity.

Used in the MTT assay to asses the viability of cells. The yellow MTT is reduced by the living cells to a water-insoluble purple formazan. In order to make a quantitative measurement the formazan is often dissolved by addition of a solubilization solution to yield a colored solution that can be measured in a spectrophotometer. The rate of color formation reflects the dynamics and intensity of the microbial metabolism.

5-Bromo-4-chloro-3-indoxyl oleate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl oleate is used in lipid metabolism disorder screening.

5-Bromo-6-chloro-3-indolyl palmitate is a magenta enzyme substrate that is widely used in the study of lipase and esterase enzymes. This compound consists of an indole ring with bromine and chlorine atoms at positions 5 and 6, respectively, and a palmitate moiety esterified to the indolyl ring. The palmitate group is a long-chain fatty acid, making this substrate particularly useful for investigating enzymes that hydrolyze ester bonds in lipids. Upon cleavage by lipases or esterases, 5-Bromo-6-chloro-3-indolyl palmitate releases a magenta-colored product, which can be easily detected and quantified to measure enzyme activity.

4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.

Water soluble substrate for firefly luciferase.

4-Chloro-3-indoxyl-1,3-diacetate is a fluorogenic or chromogenic substrate used in food testing. It is also an enzyme substrate that can be used to detect the presence of bioluminescence. 4-Chloro-3-indoxyl-1,3-diacetate has been shown to have high purity and high quality for use in diagnostic kits. 4-Chloro-3-indoxyl-1,3-diacetate is a ligand that is conjugated with other molecules to form complexes for use in fluorescence and chemiluminescence experiments.

Methylumbelliferyl butyrate is structurally based on 4-methylumbelliferone and is a MOSCERDAM® substrate. It targets the esterase enzyme.

2-Nitrophenyl 2,2',3,3',4'-penta-O-acetyl-b-D-xylobioside is an oligosaccharide that is used as a substrate for the transport of nucleic acid molecules. It can be used to study the function of membrane transport proteins and their homologues. This compound has been shown to inhibit the growth of corynebacterium glutamicum and trisaccharide emissions.

Substrate for synthesis of proluminescent bioconjugates

Chromogenic substrate targeting beta-D-xylosidase

4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-a-D-galactopyranoside is a fluorescent substrate that can be used in chemiluminescent assays. It is a high purity, high quality chemical that has been tested for culture media, environmental testing and bioluminescence. This product is an excellent choice for use in diagnostics, chromogenic substrates, enzyme substrates and food testing.

Chromogenic substrate for cellulases

4,6-Benzylidene-4-nitrophenyl-alpha-D-maltoheptaoside is a fluorogenic substrate used in the detection of alpha amylase. It reacts with alpha amylase to produce light, and can be detected by measuring the increase in light emission over time. 4,6-Benzylidene-4-nitrophenyl-alpha-D-maltoheptaoside is an excellent substrate for use in both enzymatic assays and chemiluminescence assays. It can be conjugated to antibodies or other proteins and is stable in culture media at high pH. This product has been shown to produce a signal that is proportional to the concentration of alpha amylase. The enzyme substrate comes in high purity and high quality, with a ligand that has been shown to have good binding affinity.

Chromogenic substrate for alpha-galactosidase

6-Chloro-3-indoxyl caprylate is a high quality, enzyme substrate, chromogenic substrate, bioluminescence, chemiluminescence ligand, staining and diagnostics reagent. It has CAS No. 159954-35-5 and is used in food testing, culture media and environmental testing. 6-Chloro-3-indoxyl caprylate is also a fluorogenic substrate that is used for high purity applications such as conjugation with other molecules.

Aldol® 484 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of ?-glucosidase activity. The colorless enzyme substrate is cleaved by bacteria producing ?-glucosidase in liquid or solid media, yielding intense red color and green fluorescent signal. Aldol® 484 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.

5-Bromo-4-chloro-3-indolyl a-D-maltotrioside is an enzyme substrate that shows chemiluminescence. It is used in diagnostic assays to detect bacterial and fungal infections, as well as other diseases. The test uses 5-Bromo-4-chloro-3-indolyl a-D-maltotrioside as the substrate for the enzyme horseradish peroxidase. When light is applied, the substrate reacts with hydrogen peroxide in the presence of horseradish peroxidase to form colored products that can be detected by a luminometer.

Resorufin maltotriose is an alpha-amylase substrate that releases the dye resorufin with red emission (lmax=585 nm) after enzyme reaction.

Fluorogenic substrate for β-glucosidase

Resorufin ethyl ether is a fluorescent substrate that is used to measure the activity of cytochrome P450 enzymes. It can be used in vitro to study the effects of drugs on enzyme activities and in vivo to study liver lesions. Resorufin ethyl ether is also used as a model system for studying synchronous fluorescence, which is the emission of light by two or more substances at the same time. The nitrogen atoms in resorufin ethyl ether can be substituted with other atoms such as magnesium or zinc, which will affect its activity and fluorescence properties. Resorufin ethyl ether binds to cytochrome P450 enzymes and therefore inhibits their activity. This inhibition is reversible, and it can be reversed by adding a substance that competes with the substrate for binding sites on the enzyme.

Aldol® 515 beta-D-xylopyranoside is a chemiluminescent ligand that can be used for the detection of beta-xylosidase activity to yield a red color.

Stability Moisture, Temperature sensitive Applications Used as a substrate for the detection of sialidase-like enzyme in screening of enzymes, studying physiological activities of gangliosides, and recombinant technologies. References Biorg. Med. Chem., 1, 147 (1993)

4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy--bDglucopyranoside is a compound that has been shown to interact with glutamicum and casein kinase. It is also a test compound for corynebacterium glutamicum and interacts with it by functional theory. The compound has been shown to inhibit inflammatory diseases and may be used as a potential treatment for these diseases.

Growth medium for the detection of histamine-producing bacteria in food and water samples. The medium contains an indicator that turns orange/red (absorbance at 555 nm) due to metabolites released by histidine decarboxylase. The BCM® Histamine-producing bacteria Broth contains tryptone (FT09555) and yeast extract, together with the chromogenic agent. High-level histamine-producing bacteria yield orange to red coloration in less than 2 days and even after 24 hours, while cultures of other bacteria remain yellow.

Aldol® 584 is a fluorogenic substrate that can be used for enzymatic and chemical assays. It is an important component of the Cymit Quimica Aldol Reagent Kit and other diagnostic kits. Aldol® 584 is used in immunoassays, such as ELISA, to measure the activity of serine proteases, including cathepsin D, elastase, and leukocyte elastase. This product has a CAS number of 2484873-11-0.

Applications Several novel 5-substituted 2'-deoxyuridine (dUrd) analogs were evaluated as substrates for highly purified herpes simplex virus type 1 (HSV-1)-encoded thymidine kinase (TK) derived from HSV-1 TK gene-transfected murine mammary carcinoma FM3A cells, and human platelet thymidine (dThd) phosphorylase. References Bohman, C., et al.: J. Biol. Chem., 269, 8036 (1994),

2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-galactopyranoside is a chemiluminescent substrate for the detection of beta galactosidase. It is a white to light yellow powder with a molecular weight of 564.87 and an assay of ≥ 98% by HPLC. This product has been shown to be stable when stored at 4°C for up to three months.

Substrate for alpha-galactosidase

5-Bromo-3-indolyl decanoate (BID) is a chromogenic enzyme substrate often used for the detection of lipase activity. It is hydrolyzed by lipase to produce a blue-violet product, which can be detected visually or by spectrophotometry. BID is particularly useful for the detection of microbial lipases in food and environmental samples. It is commonly used in a wide range of biochemical, biological, and environmental testing applications that require precise lipase enzyme analysis.

Inorganic phosphate targeted colorimetric probe

4-Nitrophenyl-N-acetyl-beta-D-galactosaminide is a synthetic substrate that is used in enzyme kinetics. It is a beta-galactosidase substrate that reacts with the enzyme to form a product. The rate of reaction can be measured by following the change in absorbance over time. This product will react with an acceptor, such as chondroitin sulfate, to produce an observable reaction product. 4NPBGA has been shown to be an effective carbon source for plant physiology studies and has been shown to have optimal activity at pH 6.5.

1-Naphthyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate for bacterial beta D glucosidase. It is used for measuring the enzyme activity in different types of culture media and in the detection of bacterial contamination in food and environmental samples. This product can be used to detect the presence of beta D glucosidase by measuring luminescence with a luminometer. 1NAAG can also be used as a chromogenic substrate or to conjugate with antibodies for use in immunoassays.br>

2-Chloro-4-nitrophenyl a-D-maltotrioside (2CNP) is a potent hypoglycemic agent that has been shown to decrease postprandial blood glucose levels in humans. 2CNP is a white crystalline solid that is soluble in water and ethanol. The transfer reactions of 2CNP are enhanced by benzalkonium chloride, which forms an organic complex with the drug. The optimum concentration for the hypoglycemic effect of 2CNP is determined to be 10 μM, which can be detected using an optical sensor. This compound also inhibits α-amylase and other enzymes involved in carbohydrate metabolism, leading to the accumulation of glycogen and lowering the blood glucose level.

2-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside is a chromogenic substrate used to assay the activity of enzymes that cleave N-acetyl-alpha-D-glucosaminide, such as chitinases and lysozymes. Upon cleavage by the enzyme of interest, the substrate releases a yellow-colored 2-nitrophenol that can be measured spectrophotometrically. This substrate is widely used in the food industry to monitor the activity of lysozyme in cheese, as well as in biomedical research for studying cellular and molecular mechanisms involved in conditions such as inflammation and cancer.

A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.

4-Methylumbelliferyl 2-acetamido-2-deoxy-3,6-di-O-pivaloyl-b-D-galactopyranoside is a chemiluminescent substrate that reacts with an enzyme to produce light. It is used in the detection of ATPase activity and in the diagnosis of diseases. This product has been tested for quality and environmental safety, and it does not contain any hazardous substances. It is highly pure and can be used for testing food, diagnosing diseases, or staining cells.

4-Nitrophenyl 2-O-(α-D-glucopyranosyl)-α-D-glucopyranoside is a specialized chromogenic pNP substrate featuring dual glucose-linked moieties, designed for accurate detection and quantification of specific glycosidase enzymes. Upon cleavage by target enzymes, this substrate releases 4-Nitrophenol, which can be easily monitored spectrophotometrically as a yellow color change. This advanced substrate offers enhanced specificity and sensitivity in enzyme assays, facilitating cutting-edge research in biochemistry, enzymology, and other related fields.

Aldol® 518 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color as well as red fluorescence when a fluorescence enhancer or a suitable matrix is present. Aldol® 518 beta-D-galactopyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar plates. Aldol® 518 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

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4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1->3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.

4-Methylumbelliferyl propionate is a fluorogenic substrate for esterase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl propionateas a substrate for measuring the esterase activity is used for esterase deficiency screening assays.

4-Nitrophenyl hepta-O-acetyl-1-thio-b-lactoside is an inorganic molecule that binds to the surface of bacteria. It has been used as a fingerprinting tool for a variety of organisms, including corynebacterium glutamicum. The molecule can be used to measure the metabolic activity of corynebacterium glutamicum with a technique called incremental acidification. This technique is based on the observation that the concentration of 4-nitrophenol decreases by one unit for each glucose metabolized by corynebacterium glutamicum. The use of 4NPHTBL and other primers can also be useful in diagnosing patients with autoimmune diseases such as celiac disease and inflammatory bowel disease.

6'-(O-(Carboxymethyl))-2',7'-dichlorofluoroscein 3'-(O-(N-acetyl-b-D-glucosamide)) is a chemiluminescent substrate that can be used in the detection of ligands. This product has a CAS number of 44636-76-8 and is available at a purity level of 98%. It has been tested for bacterial contamination, as well as for adverse effects on culture media and environmental testing. 6'-(O-(Carboxymethyl))-2',7'-dichlorofluoroscein 3'-(O-(N-acetyl-b-D-glucosamide)) is an enzymatic substrate that can be used in diagnostic applications such as the detection of fluorescence, staining, and bioluminescence. It has also been shown to be useful in food testing.

Chromogenic substrate for α-D-glucosidase yielding a yellow solution upon cleavage. Also used for the detection of glucansucrases and for yeast α-D-glucosidase and for studies of the crystal structure of the complexes of concanavalin A.

Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a chromogenic substrate that can be used in the detection of enzymes and other proteins. It is a fluorogenic substrate that can be used in chemiluminescence to detect and measure free radicals. Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is stable at pH 7, and has a high purity with a CAS number of 103213-40-7. This product is suitable for use as an enzyme substrate or ligand, as well as for staining and diagnostics.

2-Naphthyl caprylate is a substrate that is employed in an assays for carboxylesterase and lipase and when hydrolyzed, produces 2-naphthol, a compound that can also be identified via fluorescence analysis. By combining it with a diazonium salt, the formation of the corresponding azo dye occurs. In particular, this substrate is used to detect the extracellular lipase of Pseudomonas fluorescens B52.

4-Nitrophenyl 2,3,4-tri-O-acetyl-a-D-glucopyranoside is a high quality chemical used in the development of various diagnostic and research applications. It is also used in the production of enzymatic substrates for enzyme activity assays. 4NPTAG is a chromogenic substrate that produces chemiluminescence when it reacts with an oxidizing agent and has been shown to be very effective as a fluorogenic substrate for protein kinases. This compound has been shown to bind to ligands and can be used as a bioluminescent reporter for molecular biology applications. The CAS number for this product is 54973-14-0.

Aldol® 470 N-tosyl-L-alanine ester is a fluorogenic substrate that is used in enzyme and chromogenic assays. It is a high purity, high quality, and highly soluble substrate for chemiluminescent reactions. Aldol® 470 N-tosyl-L-alanine ester can be used to detect the presence of bacteria in culture media and as an indicator of bacterial growth. It is also used to test for the presence of different types of enzymes that catalyze the oxidation of alcohols.

4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-2-O-benzoyl-4,6-O-benzylidene a -D-mannopyranoside is a synthetic substrate for glycosidases that can be used as an alternative to other substrates. It can be used in the detection of enzyme activity and in the diagnosis of diseases such as diabetes mellitus and Gaucher's disease. 4NP3ODABAB has been shown to have high quality and purity. This substrate has not been evaluated by CAS yet.

2,4-Dinitrophenyl b-L-arabinofuranoside is a high quality chromogenic substrate for dinitrophenylase. It is a ligand that reacts with an enzyme to form a product that can be detected by various methods, including colorimetry and fluorimetry. 2,4-Dinitrophenyl b-L-arabinofuranoside has shown to be useful for the detection of organic contaminants in water and air. 2,4-Dinitrophenyl b-L-arabinofuranoside is also used as a reagent for the preparation of conjugates for immunoassays.

Naphthol AS-BI-beta-D-glucuronic acid is a β-glucuronidase substrate. The substrate is used in combination with stabilized diazonium salts such as Fast Red Violet LB salt (5-chloro-4-benzamido-2-methylbenzenediazonium chloride). Free Naphthol AS and Fast Red Violet undergo a chemical reaction to yield intensely colored azo-dyes.

Phosphatase substrate