Substratos Cromogénicos

Please enquire for more information about 5-Bromo-4-chloro-1-methyl-3-indolyl-β-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page

This product is a fluorogenic substrate that reacts with the enzyme beta-galactosidase to produce a fluorescent product. It can be used in food testing, environmental testing, and diagnostics. The CAS number for this product is 609-33-2. This product is available in high purity and high quality.

3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.

2-Naphthyl-beta-D-glucuronide sodium salt is an enzyme substrate to detect beta-glucuronidase. The enzyme cleaves the glycosidic bond in 2-naphthyl-beta-D-glucuronide to release 2-naphtol and glucuronic acid. Beta-glucuronidase activity assays are important in many fields such as cancer research, as the enzyme is expressed in some tumors and its activity can be targeted for cancer therapy. The enzyme has also an important role in drug metabolism as it recognizes and binds to drug glucuronide conjugate (as 2-naphthyl-beta-D-glucuronide) facilitating its hydrolysis to release the active part. 2-Naphthyl-beta-D-glucuronide sodium salt has a higher solubility as it is in its salt form.

6-Chloro-3-indoxyl-beta-D-glucuronic acid (cyclohexylammonium salt) is a chromogenic substrate for beta-glucuronidase yielding a salmon colored precipitate upon cleavage.

4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity

6-Bromo-2-naphthyl b-D-glucopyranoside is an enzyme substrate used in chromogenic assays with beta-glucosidases. It has been used in culture media for the identification of Legionella pneumophila.

Chromogenic substrate used to measure phospholipase C (PLC) activity

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranosyl)-3-O-(2,3,4,6-tetra -O acetyl -b D galactopyranosyl)-2 deoxy a D galactopyranoside is a high purity fluorogenic substrate for the enzyme β galactosidase. It can be used for the detection of β galactosidase in biological samples. 4NPAG can also be used as a ligand for various types of chromogenic substrates. This product is also commonly used in diagnostics and conjugates to detect β galactosidase activity or as a culture media supplement.

4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is a chromogenic substrate for glucose-6-phosphatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is used in different applications such as the Glycogen storage disease diagnosis (e.g., von Gierke disease)

Modified naphtol AS substrate used in histochemical and cytochemical staining

Please enquire for more information about 3-Indoxyl myo-inositol-1-phosphate, ammonium salt including the price, delivery time and more detailed product information at the technical inquiry form on this page

Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.

2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.

5-Bromo-3-indolyl nonanoate (BIN) is a chromogenic enzyme substrate often used for the detection of esterase activity. It is hydrolyzed by esterase to produce a blue product, which can be detected visually or by spectrophotometry. BIN is particularly useful for the detection of esterase activity in bacteria and fungi, and can be used to differentiate between different types of organisms based on their esterase activity patterns

6-Chloro-3-indoxyl-beta-D-glucuronic acid (cyclohexylammonium salt) is a chromogenic substrate for beta-glucuronidase yielding a salmon colored precipitate upon cleavage.

Substrate for beta-N-acetylglucosaminidase

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a state-of-the-art chromogenic substrate designed for rapid and sensitive enzyme activity detection. As a versatile pNP substrate, it produces a noticeable color change upon enzyme-catalyzed hydrolysis, facilitating easy monitoring and quantification of enzyme levels in research and diagnostic applications. The substrate's acetylated glucose moiety ensures specificity and stability, allowing for precise and reliable results across a range of experimental conditions.

Aldol® 518 myo-inositol-1-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-inositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms grown in liquid or solid media, yielding an intense red coloration. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also obtained. Aldol® 518 myo-inositol-1-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Substrate for a-L-arabinopyranosidase

6-Bromo-2-naphthyl beta-D-glucuronide is a substrate for beta-glucuronidase, an enzyme involved in detoxification and bacterial metabolism. Upon enzymatic hydrolysis, it releases 6-bromo-2-naphthol, which forms a detectable dye when reacted with diazonium salts. This compound is widely used in microbiology to detecting E. coli in water testing and histochemical staining. 6-Bromo-2-naphthyl beta-D-glucuronide is also been used in cancer research studies. It has been found that some tumors are related to a high activity of beta-glucuronidase, responsible to reactivate the carcinogens and promote tumor progression, developing a drug resistance to chemotherapy.

Aldol® 470 nonanoate is a chromogenic substrate with a molecular weight of 496.5 g/mol. Aldol® 470 nonanoate has been used to detect the presence of L-amino acids using a fluorogenic assay. This substrate can be used as a ligand in an enzyme-linked immunosorbent assay (ELISA) to measure the concentration of L-amino acids in food and drink samples, as well as in diagnostics for detecting fluoroquinolone resistance in bacteria. Aldol® 470 nonanoate is also used in the detection of bioluminescence and chemiluminescence reactions. CAS No.: 2484872-50-4

5-Bromo-4-chloro-3-indoxyl sulfate p-toluidine salt is a staining agent used for the detection of glucose in urine and tissues. It is also an enzyme substrate that can be used to detect the presence of lipases, amylases, esterases, phosphatases, and proteases. This product has a CAS number of 6581-23-3 and comes in high quality.

5-Bromo-4-chloro-3-indoxyl nonanoate (5BCNI) is a chromogenic substrate for the detection of alkaline phosphatase. It is soluble in most organic solvents and is stable at room temperature for up to 30 days. 5BCNI has been shown to be a high quality, sensitive and specific substrate for the detection of alkaline phosphatase in culture media, diagnostics, conjugates, environmental testing and chemiluminescence. It has also been used as an enzyme substrate in bioluminescence studies. The CAS number for 5BCNI is 133950-77-3.

Chromogenic substrate targeting alpha-amylase

4-Nitrophenyl myristate (NphOMy) is a chromogenic enzyme substrate used as a diagnosis tool for detection of β-lactamase hydrolysis activities. In the presence of certain drugs, 4-nitrophenyl myristate HSA-Tyr411-catalyzed hydrolysis seems to be inhibited.

5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-arabinofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is used in bacterial arabinan degradation assays (e.g. Bacillus spp.).

2-Chloro-4-nitrophenyl 4-O-b-D-galactopyranosylmaltoside, also known as Gal-G2-CNP, is a high quality, water soluble, chromogenic substrate that is used as an enzyme substrate and can be used in bioluminescence and chemiluminescence assays. This product is designed for use in diagnostics, culture media, food testing, environmental testing, and other research applications. 2-Chloro-4-nitrophenyl 4-O-b-D-galactopyranosylmaltoside has been tested in chromogenic assays with alpha-amylases.

2-Chloro-4-nitrophenyl b-D-maltoheptaoside is a fluorescent compound that is used in medical research for the visualization of muscle glycogen. It is also used to study the binding of molecules to proteins, as well as to measure enzyme activity. The compound has been shown to be insensitive to changes in pH and temperature, so it can be used at different pH levels and temperatures without affecting its function. 2-Chloro-4-nitrophenyl b-D-maltoheptaoside can be detected by titration calorimetry due to its high molecular weight. The molecule's affinity for carbohydrates also allows it to be used in optimization studies of carbohydrate molecules.

2-Chloro-4-nitrophenyl β-D-glucopyranoside is a chromogenic substrate for beta-glucosidases. In biochemical enzyme assays and in vitro diagnostics analysis, the substrate is used to monitor the glycosyltransferase activity. When 2-chloro-4-nitrophenyl β-D-glucopyranoside is cleaved by beta-glucosidase, 2-chloro-4-nitrophenol is released and quantified.

2,4-Dinitrophenyl-beta-D-glucopyranoside is a chromogenic substrate used to assay enzymes, such as glucosidases. These enzymes catalyze the hydrolysis of glycosidic bonds in sugars.

5-Bromo-3-indoxyl phosphate, disodium salt is an enzyme substrate that is used for environmental testing, bioluminescence, culture media, chemiluminescence, fluorogenic substrate and food testing. It is also used as a ligand for diagnostic purposes. 5-Bromo-3-indoxyl phosphate, disodium salt has CAS number 16036-59-2 and it is soluble in water. This product has high quality and purity with a conjugate of 5% phenol red. 5-Bromo-3-indoxyl phosphate, disodium salt reacts with the enzyme horseradish peroxidase to produce a red color. This product can be used to detect hydrogen peroxide in the presence of an oxidizing agent such as hydrogen peroxide or potassium permanganate. The reaction produces a blue color when the solution is viewed in daylight or under ultraviolet light.

5-Bromo-4-chloro-3-indolyl N-acetyl-a-D-neuraminic acid methyl ester is a chromogenic substrate that reacts with enzymes to produce a colored product. It can be used in diagnostic tests for the detection of various bacteria, including Salmonella and Brucella, as well as for the determination of concentration in food testing or environmental testing. 5B4C3INA can also be conjugated to other substances, such as biotin or an antibody, which allows it to be used in immunoassays.

Aldol® 470 L-alanine ester, hydrochloride is a chromogenic substrate that is used as a food testing reagent and enzyme substrate in diagnostics. It is also used to detect the presence of ligands in an environment. Aldol® 470 L-alanine ester, hydrochloride is used in chemiluminescence and bioluminescence reactions.

6-Bromo-2-naphthalenyl-b-D-mannopyranoside is a boronic acid that is used as a universal substrate for the detection of peroxidase activity. This product has been shown to be stable in most culture media and can be used to detect peroxidase activity in food, environmental, and other biological samples. 6-Bromo-2-naphthalenyl-b-D-mannopyranoside also has high purity and is conjugated with various fluorophores for use in chemiluminescence and bioluminescence assays.

3-Indoxyl caprylate is an enzyme substrate used in a variety of diagnostic applications, including the detection of various bacterial and viral infections. It is used as a substrate for beta-galactosidase, which can be detected by bioluminescence or chromogenic substrates. 3-Indoxyl caprylate also serves as a ligand for receptor binding and has been shown to bind to CD4 receptors on human T cells. 3-Indoxyl caprylate is also used as an enzyme substrate in the detection of DNA polymerases, RNA polymerases, and ATPases. This product is available with a high purity that ranges from 98% to 99%. 3-Indoxyl caprylate is soluble in water, alcohols, acetone, acetic acid, and chloroform.

4-Chloro-3-indoxyl-1,3-diacetate is a chromogenic substrate used in the identification of bile acids. It reacts with 4-chlorophenol to form a red dye. This compound is also used as a ligand for metal ions.

4-Nitrophenyl 4-O-(b-D-mannopyranosyl)-b-D-glucopyranoside is a fluorogenic substrate that can be used for the detection of β-glucosidase activity in biological samples. It is an excellent substrate for the enzyme β-glucosidase, which catalyzes the hydrolysis of ester bonds in glycosides with release of glucose and formation of a phenolic product. The 4-nitrophenol group reacts with oxygen to produce light, making it useful as a chemiluminescent or bioluminescent substrate. The conjugate may also be used to detect β-glucosidase activity in culture media by adding the substrate to the culture medium and measuring its fluorescence. 4NP4OBG has been shown to have high purity, with only trace amounts of impurities detected such as benzaldehyde and 2,6-dinitro

2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside is a compound that is commonly used as an enzyme substrate for glycogen synthase kinase (GSK). It is activated by GSK-3β and has been shown to inhibit the activity of this enzyme. This compound has also been found to have inhibitory effects on other enzymes such as carbonic anhydrase and lactate dehydrogenase. Additionally, 2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside exhibits antifungal activity against various fungi, including those resistant to other antifungal agents such as fluopyram and prothioconazole. Its mechanism of action involves interfering with ergosterol biosynthesis, an essential component of fungal cell membranes. Furthermore, this compound has potential applications in the development of new drugs targeting GSK-3β and β-catenin signaling pathways, which are implicated in various diseases including cancer and neuro

Chromogenic substrate for aryl-sulfatase. Yields a blue precipitate upon cleavage.

4-Nitrophenyl palmitate is a lipase-sensitive chromogenic substrate commonly used in the study of lipase and esterase activity. Its hydrolysis by lipases produces a yellow 4-nitrophenol, which can be easily detected spectrophotometrically, offering a quantitative measure of enzyme activity. This substrate is widely utilized in the screening of lipase-producing microorganisms and the optimization of enzyme-catalyzed reactions in various fields, including biotechnology and pharmaceutical research.

4-Nitrophenyl 2,4,6-tri-O-acetyl-3-O (2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-a-D-glucopyranoside is a chemiluminescent substrate that can be used for the detection of aminoglycosides in biological fluids. It is a high purity and high quality product with CAS No. 482743. The product has been tested for safety and purity using culture media and environmental testing. This product may be used as a bioluminescence substrate to detect the presence of aminoglycosides in samples such as urine, serum or plasma. 4NTPAGDG is also useful as a fluorogenic substrate for diagnostics and enzyme substrates for food testing.

Aldol® 518 myo-inositol-1-phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-inositol specific phospholipase C activity (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC or PI-PLC-positive microorganisms grown in liquid or solid media, yielding an intense red coloration. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also obtained. Aldol® 518 myo-inositol-1-phosphate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

2-Nitrophenyl butyrate is a chromogenic enzyme substrate that is commonly used in enzymatic assays to detect lipases and esterases. When hydrolyzed by these enzymes, the substrate yields a yellow product that can be detected spectrophotometrically at 405 nm. The advantage of using 2-nitrophenyl butyrate is its high sensitivity and specificity for detecting lipolytic and esterolytic activities. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of esterase activity in biological samples such as serum, plasma, and cell lysates.

4-nitrophenyl 2-acetamido-3-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-3,4,6-tri-O-acetyl -D -galactopyranosyl]-2 deoxy 6 O trityl b D glucopyranoside is a chemiluminescent substrate that can be used in biochemical assays. It reacts with oxygen to produce light when it is excited by a UV or blue light source. This product has been tested for purity and quality by HPLC analysis and spectroscopy.

Chromogenic substrate for β-D-fucosidase yielding a blue precipitate.

5-Bromo-4-chloro-3-indoxyl myristate is a chemiluminescent substrate that can be used in the detection of peroxide. It reacts with hydrogen peroxide to emit light, which can be detected using a luminometer. 5-Bromo-4-chloro-3-indoxyl myristate is also used for the detection of bacteria, such as Staphylococcus aureus and Escherichia coli, by staining. The compound is also used for diagnostic tests to identify diseases such as tuberculosis. 5-Bromo-4-chloro-3 indoxyl myristate is soluble in organic solvents and water, not toxic, and has a high purity level.

Chitin azure is a chromogenic substrate for chitinases. It is one of the few specific substrates for this enzyme that produces chito-oligosaccharides from chitin.

Chromogenic substrate targeting beta-galactosidase

4-Nitrophenyl b-D-galactopyranoside hydrate is a water-soluble pNP-based enzyme substrate ideal for quantifying b-galactosidase activity in a variety of sample types. This chromogenic substrate features a unique hydrate composition, which allows for enhanced solubility and stability when compared to standard pNP-galactosides. With its high sensitivity and robust performance, this substrate is widely used in biotechnology, biochemistry, and molecular biology fields, including the investigation of enzyme kinetics, characterization of glycoside hydrolase specificity, and assessment of gene expression levels.

4-Nitrophenyl 2-acetamido-3-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-3,4,6-tri-O-acetyl-2 deoxy -D-galactopyranosyl]-b D glucopyranoside is a fluorescent substrate for the enzyme β galactosidase. It can be used to test for β galactosidase activity in food products, environmental samples and diagnostics. 4NPFAPG is a conjugate of 4 nitrophenol and 2 acetamido 3 O-[2 O-(2,3,4 tri O benzoyl a L fucopyranosyl) 3,4 6 tri O acetyl 2 deoxy D galactopyranosyl] b D glucopyranose. 4NPFAPG is water soluble and has

The 4-Nitrophenyl-alpha-D-galactopyranoside is a high-quality chromogenic substrate specifically designed for the enzymatic assay of alpha-galactosidase. Upon enzyme catalysis, it yields a highly fluorescent 4-nitrophenol product, which allows easy and accurate measurement of enzyme activity in a variety of research applications, such as biochemical analysis, inhibitor screening, and enzyme kinetics studies.

Chromogenic substrate for carboxylesterase yielding a blue precipitate upon cleavage.

1-Naphthyl b-D-glucuronide sodium salt is a chromogenic substrate commonly used to assay the activity of beta-glucuronidase enzymes. It is hydrolyzed by beta-glucuronidase to yield a yellow-colored product, 1-naphthol, which can be quantified spectrophotometrically. This substrate is widely used in diagnostic tests for detection of beta-glucuronidase activity, particularly in clinical microbiology for the identification of E. coli and other enteric bacteria. With its sodium salt form, it boasts increased solubility in aqueous solutions, ensuring seamless integration into various experimental settings.

4-Nitrophenyl 2-acetamido-2-deoxy-6-O-trityl-b-D-glucopyranoside offers unmatched versatility as a chromogenic pNP substrate for detecting b-D-glucopyranosidase activity. The substrate is specifically designed to produce a yellow 4-nitrophenol product upon enzyme hydrolysis, which can be measured using absorbance spectroscopy. The unique tritylated glucopyranoside group ensures improved compound stability and selectivity while providing ease of handling. This versatile substrate is ideal for a broad range of biochemical, research, and diagnostic applications.

5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-glucosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is used in Pompe disease screening and glycogen storage disorder diagnosis.

5-Bromo-4-chloro-3-indoxyl oleate is a fluorescent substrate that is used for staining and enzyme substrate. It is a conjugate that is used in environmental testing, food testing, and diagnostics. 5-Bromo-4-chloro-3-indoxyl oleate has a CAS number of 341972-97-2 and its molecular weight is 229.5 g/mol. It's purity is > 98%, with a specific optical rotation of +147° (c=1, CHCl3). This compound can be used as a chromogenic or fluorogenic substrate in bioluminescence assays. 5BRIO3 has been shown to be an excellent ligand for the detection of DNA polymerase I activity.

Chromogenic substrate specific for α-D-mannosidase. Yields a blue precipitate upon cleavage. Identification of Listeria species.

5-Bromo-6-chloro-3-indoxyl choline phosphate is a chemiluminescent substrate for the detection of bacterial activity. It reacts with an enzyme, luciferase, which is present in a variety of living cells. The reaction produces light. This product has been tested for environmental and food safety by the manufacturer. 5-Bromo-6-chloro-3-indoxyl choline phosphate is used as a diagnostic tool to determine the presence of bacteria in humans, animals, and plants. It can be used to measure levels of bacteria in culture media, water samples, and food products. 5-Bromo-6-chloro-3-indoxyl choline phosphate can also be used as a substrate for enzymes such as phosphatases or esterases that catalyze reactions with substrates with brominated or chlorinated groups.

6-Bromo-2-naphthyl 2-acetamido-2-deoxy-beta-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.

The Aldol® 470 N-tosyl-L-alanine ester is a high purity, high quality fluorogenic substrate for the enzyme alkaline phosphatase. It is a conjugate of the tosyl group with L-alanine and has been used as a fluorogenic substrate in various food testing applications. The Aldol® 470 N-tosyl-L-alanine ester also has several other uses including in diagnostic methods, culture media, and enzyme substrates.

Aldol® 495 beta-D-glucuronic acid is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucuronidase activity. The living cells of Escherichia coli can be reliably detected in growth media containing Aldol® 495 beta-D-glucuronic acid, as beta-glucuronidase occurs almost exclusively in E. coli. Aldol® 495 beta-D-glucuronic acid can also be in GUS reporter assays. Aldol® 495 beta-D-glucuronic acid can be used in liquid culture and solid media, and is suitable for use under aerobic and anaerobic conditions. Upon exposure to active beta-glucuronidase, Aldol® 495 beta-D-glucuronic acid develops orange colour and green fluorescence.

A substrate for visualising alkaline phosphatase activity. Produces a red colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.

5-Bromo-4-chloro-3-indolyl b-D-fucopyranoside is a chromogenic substrate used is for the enzyme beta-fucosidase.

Used in biological studies as a chromogenic substrate for β-galactosidase; red color.Chlorophenol red indicates changes of pH in the range of 4.8 to 6.7 by turning the solution from yellow (at pH 4.8) to violet (at pH 6.7). Converted as signalogenic moiety of an enzyme substrate, chlorophenol red beta-D-galactopyranoside has a good proven sensitivity in the detection of beta-galactosidase activity in transfected mammalian cells, when compared to ONPG (o-nitrophenyl-beta-D-galactopyranoside) (Eustice, 1991)

Aldol® 518 alpha-D-glucopyranoside is a fluorogenic enzyme substrate used for detection of alpha-D-glucosidase activity. The colorless enzyme substrate is cleaved by alpha-D-glucosidase, generating red fluorescent signal and in some conditions, an orange colour in the visible spectrum can get formed, staining bacterial colonies in faint orange coloration. Aldol® 518 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.

Calcium 2-naphthylphosphate, which is the calcim salt of 2-naphthylphosphate, is a chromogenic substrate to measure alkaline phosphatase activity. After enzymatic cleavage, the product yielded (2-naphthol) generates a red-colored complex upon reaction with a diazonium salt like Fast Blue RR or Fast Garnet GBC.

Aldol® 515 caprylate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C8 esterases, yielding development of red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 caprylate can be used in enzyme assays and live bacterial cultures. Aldol® 515 caprylate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

6-Chloro-3-indolyl butyrate is a chromogenic substrate for lipases. Use in lipid digestion and metabolism studies, it produces a salmon-pink color when 6-chloro-3-indolyl is released after enzymatic cleavage.

Methyl 4-nitrophenyl hexylphosphonate is a phosphonate inhibitor of the enzyme serine esterase. It has been shown to be more potent than other irreversible inhibitors such as ethyl 4-nitrophenyl hexylphosphonate and methyl 4-nitrophenoxybutyl phosphate. This compound is an immobilized form of the methyl 4-nitrophenyl hexylphosphonate that is immobilized on an inert surface, such as silica gel, via a covalent bonding process. The immobilization of the molecule ensures that it will not leach from the surface and is stable over time. The molecular weight of this substance is 411.5 Daltons and its molecular formula is C8H15NO4P. This substance has a diameter of 0.8 nm and can be found in crystalline form with a melting point of 143 degrees Celsius at atmospheric pressure. Methyl 4-nitrophenyl

2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.

6-Bromo-2-naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.

2-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to detect β-galactosidase activity. This specific substrate is one of the most commonly used substrates for detecting β-galactosidase activity in diagnostic assays. It reacts with the enzyme to produce a blue color and has a fluorescence emission maximum at about 480 nm. The product is available in high purity and high quality and it is also an enzyme substrate.

4-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-4-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-b-D-glucopyranoside is modified acetylated chromogenic substrate designed for the detection and quantification of enzyme activity. This highly specific substrate is cleaved by enzymes, releasing a yellow 4-nitrophenol product that can be easily measured spectrophotometrically. This allows for accurate and sensitive analysis of enzyme kinetics, inhibition studies, and substrate specificity determination.

5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-xylosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is used in bacterial xylan metabolism studies and plant cell wall degradation assays.

2-Naphthyl-α-D-glucopyranoside is a substrate for α-glucosidase. 2-Naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding reddish-brown azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

Chromogenic substrate for α-galactosidase yielding a blue precipitate. Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifido bacteria species from Lactobacillus species.

5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside is a crystalline compound that is used in microbiological studies. It inhibits the growth of organisms by binding to active enzymes and preventing the formation of ATP. The crystalline form of 5-bromo-4-chloro-3 indoxyl beta D cellobioside has been shown to be effective against recombinantly produced proteins in proteomic studies. This compound has also been shown to inhibit the activity of hydrolytic enzymes, which are involved in both carbohydrate and protein metabolism. 5-Bromo-4 chloro 3 indoxyl beta D cellobioside has been found to be expressed at endogenous levels in a number of organs, including the liver, kidney, brain, and spleen.

Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their β-galactosidase activity.

Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol® substrates can be used under aerobic and anaerobic conditions.

[2-Methoxy-4-(2-nitrovinyl)phenyl] beta-D-galactopyranoside is a chromogenic substrate (505 nm) for β-glucosidase.

Aldol® 515 caprylate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C8 esterases, yielding development of red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 caprylate can be used in enzyme assays and live bacterial cultures. Aldol® 515 caprylate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ coliform culture media. About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products and shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants. The sodium salt has advantages over the cyclohexylammonium salt as being non-electrostatic and possessing a naturally-occurring cation.