Fluorogenic Substrates

4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.

Methylumbelliferyl butyrate is structurally based on 4-methylumbelliferone and is a MOSCERDAM® substrate. It targets the esterase enzyme.

Resorufin maltotriose is an alpha-amylase substrate that releases the dye resorufin with red emission (lmax=585 nm) after enzyme reaction.

Fluorogenic substrate for β-glucosidase

Resorufin ethyl ether is a fluorescent substrate that is used to measure the activity of cytochrome P450 enzymes. It can be used in vitro to study the effects of drugs on enzyme activities and in vivo to study liver lesions. Resorufin ethyl ether is also used as a model system for studying synchronous fluorescence, which is the emission of light by two or more substances at the same time. The nitrogen atoms in resorufin ethyl ether can be substituted with other atoms such as magnesium or zinc, which will affect its activity and fluorescence properties. Resorufin ethyl ether binds to cytochrome P450 enzymes and therefore inhibits their activity. This inhibition is reversible, and it can be reversed by adding a substance that competes with the substrate for binding sites on the enzyme.

4-Methylumbelliferyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy--bDglucopyranoside is a compound that has been shown to interact with glutamicum and casein kinase. It is also a test compound for corynebacterium glutamicum and interacts with it by functional theory. The compound has been shown to inhibit inflammatory diseases and may be used as a potential treatment for these diseases.

L-Proline 7-amido-4-methylcoumarin hydrobromide is a fluorogenic substrate that is used to measure esterase and peptidase activity in a variety of animal and microbial sources. This compound has been shown to have a lipase activity. L-Proline 7-amido-4-methylcoumarin hydrobromide is also used as an enzyme preparation and as an enzyme source.

A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.

4-Methylumbelliferyl 2-acetamido-2-deoxy-3,6-di-O-pivaloyl-b-D-galactopyranoside is a chemiluminescent substrate that reacts with an enzyme to produce light. It is used in the detection of ATPase activity and in the diagnosis of diseases. This product has been tested for quality and environmental safety, and it does not contain any hazardous substances. It is highly pure and can be used for testing food, diagnosing diseases, or staining cells.

4-Methylumbelliferyl alpha-D-mannopyranoside (4-MU-a-D-Man) is a fluorogenic substrate used in activity assays for alpha-mannosidase. When it is hydrolyzed by alpha-mannosidase, it produces a fluorescent compound, 4-methylumbelliferone (4-MU). 4-MU-a-D-Man is often used in medical research to monitor the activity of alpha-mannosidase, which is important in lysosomal storage diseases.  In addition, 4-Methylumbelliferyl alpha-D-mannopyranoside has been used to study the the activity of concanavalin A, a lectin that specifically binds to alpha-D-mannopyranosyl and alpha-D-glucopyranosyl residues on various glycoproteins. The use of this fluorogenic enzyme substrate permits study the degree of alpha-D-mannosidic linkage cleavage by the enzyme.

6'-(O-(Carboxymethyl))-2',7'-dichlorofluoroscein 3'-(O-(N-acetyl-b-D-glucosamide)) is a chemiluminescent substrate that can be used in the detection of ligands. This product has a CAS number of 44636-76-8 and is available at a purity level of 98%. It has been tested for bacterial contamination, as well as for adverse effects on culture media and environmental testing. 6'-(O-(Carboxymethyl))-2',7'-dichlorofluoroscein 3'-(O-(N-acetyl-b-D-glucosamide)) is an enzymatic substrate that can be used in diagnostic applications such as the detection of fluorescence, staining, and bioluminescence. It has also been shown to be useful in food testing.

Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a chromogenic substrate that can be used in the detection of enzymes and other proteins. It is a fluorogenic substrate that can be used in chemiluminescence to detect and measure free radicals. Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is stable at pH 7, and has a high purity with a CAS number of 103213-40-7. This product is suitable for use as an enzyme substrate or ligand, as well as for staining and diagnostics.

Substrate for aminopeptidase a or the assay of γ-glutamyl transferase yielding a blue fluorescent solution upon cleavage.

N-Acetyl-3,7-dihydroxyphenoxazine is a model system that has been shown to have receptor activity. The rate constant of this compound was determined by measuring the binding affinity for recombinant cytochrome P450. This compound also has an angiogenic process and may be useful in the treatment of cancer.

Fluorogenic substrate for phosphatidylinositol-specific phospholipase C. Isolation and identification of Listeria monocytogenes, bacteria of the Bacillus cereus group, yeasts and molds. See also B-7404.

Glycine 7-amido-4-methylcoumarin hydrobromide is a chromogenic substrate that can be used for the detection of alkaline phosphatase. It reacts with the enzyme to produce a blue color, which can be measured spectrophotometrically. The product is also used as a ligand in cell culture media and as a diagnostic agent in environmental testing. It has been shown to be an excellent substrate for chemiluminescence reactions with luminol and luciferin, and bioluminescence reactions with luciferase. Glycine 7-amido-4-methylcoumarin hydrobromide is available from several suppliers at different prices, but we are able to offer it at a competitive price due to our high quality standards. The following product description was generated for 6-Fluoro-3-indoxyl-beta-D-galactopyranoside: Rif

A substrate used for the diagnosis of Niemann-Pick A/B.

A substrate used for the diagnosis of Mucopolysaccharidosis (MPS IV A).

Substrate of galactose-6-sulphate sulfatase ; for diagnosing MPS IV A

Ethyl umbelliferone-3-carboxylate-a-D-glucopyranoside is a fluorogenic substrate used for detecting alpha-glucosidase activity in enzymatic assays. When alpha-glucosidase cleaves , the glycosidic bond, umbelliferone-3-carboxylate is released, which exhibits strong fluorescence. This substrate is commonly utilized in glycobiology research, diagnostic tests, and high-throughput screening of enzyme inhibitors.