Fluorogenic Substrates
In this category, you will find a comprehensive selection of fluorogenic substrates designed for enzymatic catalysis. These substrates are used with various types of enzymes and are capable of producing color and fluorescence upon reaction. Fluorogenic substrates are essential tools in biochemical and molecular biology research, enabling the detection and quantification of enzyme activity with high sensitivity and specificity. They are widely used in assays for studying enzyme kinetics, screening for enzyme inhibitors, and monitoring cellular processes. At CymitQuimica, we provide high-quality fluorogenic substrates to support your research, ensuring reliable and accurate results in your enzymatic assays.
Products of "Fluorogenic Substrates"
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4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside
4-Methylumbelliferyl 3,4,6-tri-O-acetyl-2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)-b-D-galactopyranoside is a fluorogenic substrate that is used for the detection of β D -galactosidase. β D -Galactosidase is an enzyme that breaks down lactose in milk and is used as a diagnostic tool for identifying lactose intolerance. This substrate can be used as a conjugate in immunoassays to detect antibodies against the enzyme or it can be used to measure the activity of the enzyme in food samples. It also can be used to measure α1,3 galactosyltransferase activity in colorectal cancer cells or bacteria.Purity:Min. 95%Molecular weight:922.9 g/mol4-MUNANA
CAS:Fluorogenic substrate to assay the activity of neuraminidase. 4-Methylumbelliferone is released from 4-MUNANA by neuraminidase and can be detected fluorometrically with excitation at 365-380 nm and emission at 445-454 nm. Also applicable for fluorescent staining of sialidases in PAGE.Formula:C21H24NNaO11Purity:Min. 96%Color and Shape:White PowderMolecular weight:489.41 g/molRef: 3D-EM05195
Discontinued product3-(7-Hydroxycoumarin-3-carboxamido)propyl 4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside
3-(7-Hydroxycoumarin-3-carboxamido)propyl 4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside is a fluorogenic substrate that is used for the detection of beta-galactosidase. It has been shown to be an efficient substrate for the enzyme beta galactosidase and can be used to detect this enzyme in cell culture media and other diagnostic applications. 3-(7-Hydroxycoumarin-3-carboxamido)propyl 4-O-(b-D-galactopyranosyl)-b-D-glucopyranoside can also be used as a chromogenic substrate, which is added to a reaction mixture containing beta galactosidase, where it is hydrolyzed by the enzyme to produce a blue color. This product also has food testing and environmental testing applications.Purity:Min. 95%4-Methylumbelliferyl beta-D-lactoside
CAS:Fluorogenic substrate for beta-galactosidaseFormula:C22H28O13Purity:Min. 98 Area-%Color and Shape:White PowderMolecular weight:500.45 g/mol4-Methylumbelliferyl 6-thio-palmitate-b-D-glucopyranoside - Moscerdam™ biochemical purity
CAS:A fluorogenic substrate for the assay of palmitoyl-protein thioesterase used in the diagnosis of infantile neuronal ceroid lipofuscinosis.Formula:C32H48O8SPurity:Min. 95%Color and Shape:White PowderMolecular weight:592.78 g/mol4-Methylumbelliferyl-a-D-mannopyranoside-6-phosphoethanolamine
4-Methylumbelliferyl-a-D-mannopyranoside-6-phosphoethanolamine is a synthetic fluorogenic substrate that can be used in the detection of phosphatases. This substrate is a chromogenic and fluorogenic substrate that reacts when it is cleaved by enzymes. It has been shown to work with a number of different enzyme types, including esterases, phosphatases, and proteases. 4-Methylumbelliferyl-a-D-mannopyranoside-6-phosphoethanolamine has demonstrated high quality for use in bioluminescence, food testing, and environmental testing.4Methylumbelliferyl - a - D - mannopyranoside - 6 - phosphoethanolamine has been assigned CAS No. 81771-07-2.Purity:Min. 95%5-Octanoylaminofluoroscein di-b-D-glactopyranoside
5-Octanoylaminofluoroscein di-b-D-glactopyranoside is a chemiluminescent substrate that can be used for the detection of peroxidase activity. It is a bifunctional substrate, which means it has two reactive groups: one at the 5 position and one at the 8 position. The 5-octanoylaminofluorescein reacts with hydrogen peroxide in a 1:1 ratio, emitting light at 490 nm. The 8-position amine reacts with 2,2'-azino-bis(3-ethylbenzthiazoline)6 sulphonic acid (ABTS) to produce an orange color product that acts as a chromogen. The substrate can be used in diagnostics, food testing, and enzyme substrates.Purity:Min. 95%Molecular weight:797.8 g/mol4-Methylumbelliferyl 2-acetamido-2-deoxy-a-D-glucopyranoside-6-sulfate sodium salt - Moscerdam™ biochemical purity
CAS:A substrate used for the diagnosis of Sanfilippo disease type D (MPS IIID).Formula:C18H20NNaO11SPurity:Min. 95%Color and Shape:White PowderMolecular weight:481.41 g/mol4-Trifluoromethylumbelliferyl β-D-galactopyranoside
CAS:4-Trifluoromethylumbelliferyl β-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase . Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl β-D-galactopyranoside is commonly used in bacterial detection and in LacZ gene expression assays.Formula:C16H15F3O8Purity:Min. 95%Color and Shape:PowderMolecular weight:392.28 g/mol4-Methylumbelliferyl phosphate, bis(2-amino-2-methyl-1,3-propanediol) salt
CAS:Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases.Formula:C18H31N2O10PPurity:Min. 98.0 Area-%Molecular weight:466.43 g/mol4-Methylumbelliferyl-2-O-(a-L-fucopyranosyl)-a-L-fucopyranoside
4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis.Purity:Min. 95%Color and Shape:White PowderMolecular weight:468.45 g/mol4-Methylumbelliferyl 2-azido-2-deoxy-3-O-(tetra-O-acetyl-b-D-galactopyranosyl)-4,6-O-benzylidene-a-D-galactopyranoside
CAS:4-Methylumbelliferyl 2-azido-2-deoxy-3-O-(tetra-O-acetyl-b-D-galactopyranosyl)-4,6-O-benzylidene a D galactopyranoside is a chemiluminescent substrate for detecting ligands. It has been used to detect the presence of bacteria, fungi, and parasites in food samples and as an indicator for enzymatic activity. This product is an excellent fluorescent substrate for the detection of nucleic acids such as DNA. This product is a conjugate with high purity, which makes it suitable for use in various types of applications.Formula:C37H39N3O16Purity:Min. 95%Molecular weight:781.72 g/mol4-Methylumbelliferyl phosphate, dilithium salt
CAS:4-Methylumbelliferyl phosphate, dilithium salt is a fluorescent probe that is used for the detection of phosphatases. The fluorescence of this compound is inversely proportional to the activity of phosphatases. This compound can be used as a substrate for phosphatases, which are enzymes that break down phosphate groups from compounds such as ATP, ADP, and AMP. 4-Methylumbelliferyl phosphate, dilithium salt reacts with surface antigens on cells and shows reproducible results when screened with monoclonal antibodies.Formula:C10H7Li2O6PMolecular weight:268.02 g/molRef: 3D-M-5920
1gTo inquire5gTo inquire250mgTo inquire500mgTo inquire2500mgTo inquire-Unit-ggTo inquire4-Trifluoromethylumbelliferyl Tetra-O-acetylated α-D-N-Acetylneuraminate Methyl Ester
CAS:4-Trifluoromethylumbelliferyl Tetra-O-acetylated alpha-D-N-Acetylneuraminate Methyl Ester is a chemiluminescent substrate that is often used in enzyme and immunoassays. It is a high purity, high quality chemical with a CAS number of 195210-09-4. The 4-Trifluoromethylumbelliferyl Tetra-O-acetylated alpha-D-N-Acetylneuraminate Methyl Ester is used in the detection of bacterial infections and can be used to measure the activity of enzymes such as lysozyme and peroxidase. This product has been tested for its ability to induce bioluminescence in bacteria grown in culture media, as well as for its ability to cause fluorescence or staining in cells or tissues. The 4TUMBTM Tetra O AcetylatedFormula:C30H32F3NO15Purity:Min. 95%Molecular weight:703.57 g/mol6,8-Difluoro-4-methylumbelliferyl b-D-cellotrioside
6,8-Difluoro-4-methylumbelliferyl b-D-cellotrioside is a ligand that binds to active sites of enzymes such as beta-glucuronidase and beta-glucosidase. It is used in diagnostics for the detection of bacteria in culture media and food testing where it can be used as a chromogenic or fluorogenic substrate. 6,8-Difluoro-4-methylumbelliferyl b-D-cellotrioside is also used to detect the presence of bacteria and fungi in environmental samples. It has a high purity and conjugate with other molecules for use in fluorescence microscopy.Purity:Min. 95%Color and Shape:White to off-white powder.Molecular weight:698.57 g/mol4-Trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid
CAS:4-Trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid is a fluorescent chemiluminescent substrate that is used in the detection of beta-galactosidase activity. Beta-galactosidase catalyzes the hydrolysis of 4-trifluoromethylumbelliferyl a-D-N-acetylneuraminic acid to form 4-trifluoromethylumbelliferone and D-galactose, which emits light at approximately 480 nm. This reaction can be detected by fluorescence spectrophotometry using a fluorometer or photometer. 4TFUNA is not toxic, does not inhibit enzymes, and has been tested for toxicity and mutagenicity using bacteria and mammalian cells.Purity:Min. 95%Molecular weight:521.39 g/mol4-Methylumbelliferyl a-L-idopyranosiduronic acid sodium salt
CAS:PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI179383. 4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).Formula:C16H15NaO9Purity:Min. 98 Area-%Color and Shape:White PowderMolecular weight:374.27 g/mol4-Methylumbelliferyl-β-D-xylopyranoside
CAS:Fluorogenic substrate for β-D-xylosidase. Identification within the family of Enterobacteriaceae.Formula:C15H16O7Purity:Min. 99.0 Area-%Molecular weight:308.29 g/mol4-Trifluoromethylumbelliferyl b-D-xylopyranoside
4-Trifluoromethylumbelliferyl b-D-xylopyranoside (4TUF) is a fluorescent probe that can be used to monitor the activity of protein kinases. The fluorescence intensity of 4TUF changes in response to the phosphorylation state of the target protein. This probe can be used in computational and experimental investigations of protein kinase signalling pathways, as well as for optimizing drug design.Purity:Min. 95%N-Succinyl-Ala-Phe-Lys 7-amido-4-methylcumarin acetate (salt)
CAS:Controlled ProductFluorogenic substrate targeting plasminFormula:C34H43N5O10Purity:Min. 95%Molecular weight:681.7 g/mol
