Apoptosis

Applications Labelled Isonicotinamide is an isomeric analogue of nicotinamide and a metabolite of isonicotinic thioamide. Isonicotinamide strongly inducde apoptosis in human acute myelomonocytic leukemia cells, HL-60. References Taguchi, H.: Furi Rajik. Rin., 14, 23 (1999); Golovinski, E. et al.: Comp. Rend. Acad. Bulg. Sci., 21, 375 (1968);

Indirubin (Couroupitine B) is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 uM and 0.6 uM.

Theophylline monohydrate (Quibron) appears to inhibit phosphodiesterase and prostaglandin production, regulate calcium flux and intracellular calcium

(Z)-Guggulsterone triggers apoptosis in prostate cancer cells and blocks angiogenesis by inhibiting VEGF pathways.

XX-650-23 is a CREB inhibitor that induces apoptosis and cell cycle arrest in AML cells and can be used to study acute myeloid leukemia (AML).

Applications Deoxycholyltaurine rescues human colon cancer cells from apoptosis by activating EGFR-dependent PI3K/Akt signaling. References Cheng, K., et al.: Biochem. J., 330, 353 (1998), Cheng, K., et al.: Cancer Res., 63, 6744 (2003), Bellacosa, A., et al.: Adv. Cancer Res., 94, 29 (2005), Cheng, K., et al.: Biochem. Pharmacol., 70, 1035 (2005), Fang, D., et al.: J. Biol. Chem., 282, 11221 (2007),

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep CASP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep CASP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep CASP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep CASP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Oxysophoridine protects against brain ischemia and heart damage through antioxidant, anti-apoptotic, and anti-inflammatory effects.

CQ-ER is a Coumarin-quinazolinone-based photosensitizer targeting the endoplasmic reticulum (ER). It induces ferroptosis, thereby enhancing photodynamic therapy (PDT).

Targapremir-210 (TGP-210) is a miRNA-210 (miRNA-210) inhibitor that inhibits pre-miR-210 processing and induces apoptosis in breast cancer cells.

CP-31398 stabilizes the active conformation of p53 in cancer cells with either mutated or wild-type p53, enhancing its activity. Additionally, CP-31398 upregulates target genes of p53 such as p21WAF1/Cip1 and KILLER/DR5. This compound possesses tumor-suppressive properties.

'CCT128930, potent Akt2 inhibitor (IC50=6 nM), 28x more selective over PKA.'

Jaceosidin: anti-oxidant, anti-inflammatory, anti-cancer, immunosuppressive; affects CYP1A2/UGT1A1/UGT1A7 metabolism.

Evofosfamide (TH-302) is a hypoxia-activated prodrug that releases Br-IPM in tumors to cause DNA damage and potentially induce apoptosis.

4-Methyldaphnetin (DHMC) is a potent inhibitor of lipid peroxidation and scavengers of superoxide anion radicals and of aqueous alkylperoxyl radicals.

Corilagin has anti-inflammatory activity.

Applications Labelled analogue of Sulindac Sulfone, an active metabolite of Sulindac. Inducer of apoptosis independent of cyclooxygenase inhibition. Inhibits cell growth by inducing apoptosis. References Piazza, G.A. et al.: Cancer Res., 55, 3310 (1995);

Applications tert-Butyl 3-Aminopropyl(methyl) Carbamate acts as a reagent for dihydroquinazoline derivatives preparation and antitumor activities, induction of apoptosis in human lung adenocarcinoma A549 cells by T-type calcium channel antagonist. References Choi, D. L., et al.: Bioorg. Med. Chem. Lett. 24, 1565 (2014); Byun, J. S., et al.: Bioorg. Med. Chem. Lett., 26, 1073 (2016);

NHWD-870 selectively inhibits BET bromodomains BRD2-4, BRDT; potent anti-cancer effect by inducing apoptosis, halting cell growth.

Volasertib (BI 6727) (BI-6727) is a potent inhibitor of PLK1 (IC50: 0.87 nM), inducing mitotic arrest and apoptosis.

Sandalore boosts hair growth, reduces cell death, and raises IGF-1, acting on olfactory receptor OR2AT4.

WK88-1, an inhibitor of Hsp90, effectively induces the degradation of various oncogenic signaling molecules, including EGFR, ErbB2, and ErbB3, in the gefitinib-resistant H1975 cell line. This leads to subsequent growth arrest and apoptosis.

Applications Solasodine Hydrochloride enhances the effect of As2O3 in inducing apoptosis in HeLa cell, which is related to its inhibiting telomerase activity in HeLa cells. References Ai, J., et al.: Chin. J. Endemiol., 30, 279 (2011)

SFI003 is a novel SRSF3 inhibitor that exerts anticancer activity against colorectal cancer by modulating the SRSF3 / DHCR24 / ROS axis and driving apoptosis in

ERK1/2 inhibitor 11 (compound L6) is a dual inhibitor of ERK1/2 that promotes the accumulation of DSBs and the degradation of ERK1/2 expression. This compound decreases BCL-2 levels and induces DNA damage by inhibiting PARP and ERK1/2. Additionally, ERK1/2 inhibitor 11 activates caspase 3 to induce apoptosis.

MIM1 (Inhibitor of Mcl-1) is an myeloid cell factor 1 (Mcl-1) inhibitor.

Ferroptosis-IN-6 is an efficient ferroptosis inhibitor, protecting cells from cell death induced by ferroptosis inducers, and inhibiting STY-BODIPY oxidation.

4E1RCat is a dual inhibitor of eIF4E:eIF4 g and eIF4E:4E-BP1 interaction. And it inhibits the binding of eIF4 g to eIF4E with IC50 of 3.2 μM.

BCL6-IN-7 (BCL6 inhibitor-7) is a BCL6 corepressor-interaction inhibitor with potential antitumor activity, used in lymphoma research.

2',3'-Dehydrosalannol deters Spodptera litura and inhibits TNBC cell growth by blocking cathepsin signals.

Atezolizumab is an antibody inhibitor, a humanized monoclonal antibody, IgG1, which targets PD-L1. Atezolizumab has antitumor effect. Cost-effective and quality-assured.

KY-05009 blocks TGF-β1 EMT in lung cancer cells, inhibits TNIK, Wnt genes, induces apoptosis, and is an ATP-competitive TNIK inhibitor (Ki = 100 nM).

SCR7, a specific DNA Ligase IV inhibitor, blocks nonhomologous end-joining (NHEJ).

FMP is a platinum (IV) complex. It significantly upregulates the expression of γ-H2AX and p53, enhances ROS production, and markedly increases the expression of apoptosis (Apoptosis) related proteins (DR5, Fas, caspase-8, Cyt-c, caspase-3, cleaved-PARP1, Bax). FMP exhibits antiproliferative activity against breast cancer.

EGFR-IN-134 (compound 3f) is a triazolo[3,4-a]quinoline derivative and functions as a potent EGFR inhibitor with an IC50 of 0.023 µM. It induces apoptosis and necrosis in cells, and causes cell cycle arrest in the G2/M and pre-G1 phases. EGFR-IN-134 modulates the expression of apoptosis-regulating proteins by downregulating anti-apoptotic protein Bcl2 and upregulating pro-apoptotic proteins p53, Bax, and caspases 3, 8, and 9, demonstrating significant antiproliferative and anticancer activities.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Smad2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Smad2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Smad2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Smad2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Vatalanib hydrochloride (PTK787 hydrochloride) is an orally available and highly potent tyrosine kinase (VEGF) inhibitor that reduces the number and size of Aβ plaques in the cortex of 5xFAD mice, which may be useful in the study of Alzheimer's disease and cancer.

Eriocalyxin B triggers apoptosis in pancreatic cancer via caspase, p53, and STAT3 inhibition.

CCT 137690 is a highly specific and oral-available aurora kinase inhibitor, for aurora A(IC50=15 nM ), B(IC50=25 nM) and C(IC50=19 nM).

Deguelin ((-)-Deguelin) is a PI3K/AKT Inhibitor, which is a natural product isolated from plants in the Mundulea sericea family.

Ac-IETD-CHO is a potent, reversible inhibitor of granzyme B and caspase 8, which also impedes Fas-mediated apoptosis, hemorrhagic conditions, and liver failure.

Minodronic acid (YM-529) is a P2X2/3 antagonist with anticancer properties, inhibiting cell growth and metastasis, and used in osteoporosis research.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alda-1 activates ALDH2*1 and ALDH2*2, permeates cells, and targets mitochondrial aldehyde dehydrogenase 2.

Palmatine, also known as Burasaine, inhibits dopamine production and may treat flavivirus, jaundice, dysentery, hypertension, and liver issues.

FLLL32 is an effective JAK2/STAT3 inhibitor (IC50 of <5 μM).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Bcl2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Bcl2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Bcl2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Bcl2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Prim-O-glucosylcimifugin (Cimifugin 7-glucoside) can inhibit the proliferation of SMC stimulated by TNF-alpha, increase the proportion of G0/G1 phase.

Necrostatin 2 is a RIPK1 inhibitor that can be used to study inner ear disorders such as sudden deafness, dizziness and tinnitus.

Manumycin A is an antibiotic with anticancer activity and is a TrxR-1 inhibitor.

SMBA1 is a Bax agonist with antitumor activity that can induce cell cycle arrest and apoptosis in malignant glioma cells.

(E)-Ferulic acid ((E)-Coniferic acid) causes the phosphorylation of β-catenin, resulting in proteasomal degradation of β-catenin and increases the expression of

Dihydrokaempferol (Aromadendrin) is a natural product. It induces apoptosis and inhibits Bcl-2 and Bcl-xL expression, possesses anti-inflammatory activity.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian ANXA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian ANXA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian ANXA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian ANXA5 in the samples is then determined by comparing the OD of the samples to the standard curve.

OSU-T315 (OSU-T315 (1,5-regioisomer)) (1,5-regioisomer) is a ILK inhibitor.

PU02 (6-[(1-Naphthalenylmethyl)thio]-9H-purine) is a potent and selective antagonist of 5-HT3 receptor.

Eeyarestatin I blocks ER protein degradation, hinders p97/atx3 processes, and induces anticancer proteins.

Nebularine, a purine nucleoside analog, inhibits DNA synthesis, induces apoptosis, and has broad antitumor effects, especially against lymphoid cancers.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1a in the samples is then determined by comparing the OD of the samples to the standard curve.

BAY1082439, an orally bioavailable, selective inhibitor of PI3Kα/β/δ, demonstrates high efficacy in inhibiting the growth of Pten-null prostate cancer [1][2].

C-DIM12 boosts Nurr1 gene expression, heightens Nurr1 protein in neurons, and improves survival against 6-OHDA.

Ciglitazone: potent PPARγ agonist, EC50 3 μM, oral hypoglycemic; reduces insulin, blood pressure, Th17 cells, VEGF; halts gastric cancer growth.

3′-Hydroxyflavanone is a cyclized flavonoid with anti-tumor properties that induces apoptosis in HeLa cells.

TC ASK 10 is a potent, selective and orally active inhibitor of apoptosis signal-regulating kinase 1 (ASK1,IC50 = 14 nM). The IC50 value for ASK2 is 0.51 μM.

Ciprofloxacin monohydrochloride (Bay-09867 hydrochloride) is a broad-spectrum antimicrobial carboxyfluoroquinoline.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CASP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CASP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CASP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CASP8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Ac-Pro-Gly-Pro-OH can be used as a CXCR2 agonist with bactericidal and anti-inflammatory activity for the study of sepsis and lung inflammation.

ZSQ836 is a dual covalent inhibitor of CDK12/CDK13 with oral bioactivity, displaying an EC50 value of 32 nM against CDK12. This compound can induce cell apoptosis (apoptosis) and demonstrates in vivo anticancer properties. ZSQ836 is applicable for research in ovarian cancer.

Dehydrocavidine inhibits tumor growth by inducing apoptosis in MCF-7 cells, regulating Bax/Bcl-2, and activating caspases.

DPN (Diarylpropionitrile) is an selective agonist of estrogen receptor β (ERβ) .

SCH79797 dihydrochloride is a PAR1 antagonist with IC50 70 nM, Ki 35 nM, antiproliferative, and pro-apoptotic properties.

Mcl1-IN-9 is a potent myeloid cell leukemia-1 (Mcl-1) Inhibitor with an IC50 of 446 nM in reengineered BCR-ABL+ B-ALL cells and a Ki of 0.03 nM.

Morroniside regulates liver inflammation, lipid metabolism, protects against diabetic kidney damage, and guards the brain from ischemic injury.