Apoptosis

Forodesine inhibits lymphocyte growth and induces apoptosis in leukemic cells; oral purine nucleoside phosphorylase blocker; IC50: 0.48-1.57 nM.

1. Liensinine, a kind of isoquinoline alkaloid, can antagonize the ventricular arrhythmias.

Erastin2 is an iron death inducer that induces cell death by binding to the lipophilic free radical trapping antioxidant ferrostatin-1 or the iron chelator DFO.

Helichrysetin shows mild anti-HIV-1 activity and potential as an anticancer agent with cytotoxic effects on A549, MCF-7, Ca Ski, HT-29 cells.

SM-164 Hydrochloride: cell-permeable, binds to XIAP (BIR2/3 domains), IC50 of 1.39 nM, potent XIAP antagonist.

Applications 6-Chloroisatin (cas# 6341-92-0) is a useful research chemical. Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package

UM4118 (N-quinolin-8-ylpyridine-2-carboxamide) is a copper ion carrier used in the study of acute myeloid leukemia.

Belantamab (GSK2857914) is a humanized IgG1 monoclonal antibody targeting BCMA, used for creating conjugates like mafodotin.

PD168393 is an irreversible EGFR inhibitor (IC50: 0.70 nM), irreversibly alkylate Cys-773; inactive against PKC, FGFR, PDGFR, and insulin.

CCCP blocks STING activation, TBK1, IRF3 signaling, and IFN-β production by disrupting MMP.

Applications Selonsertib is an apoptosis signal-regulating kinase 1 (ASK1) inhibitor with potential anti-inflammatory, antineoplastic and anti-fibrotic activities. Selonsertib interacts with the catalytic kinase domain of ASK1 and prevents its phosphorylation and activation. As a result, the expression of genes involved in fibrosis, cellular proliferation, and apoptosis are down regulated. References Nelson, C. H. et al.: Clin Pharmacol Ther. 98, 630 (2015); Lin, J. H., et al.: Nephron. 129, 29 (2015)

IITZ-01 is potent autophagy inhibitor with single-agent anticancer activity ( IC50 of 2.62 μM for PI3Kγ.)

Tadalafil (IC-351) is a carboline-based compound with vasodilatory activity.

Compound Rh2 (Anticancer agent 133) is a cytotoxic and antimetastatic agent that induces cell cycle arrest, apoptosis, and autophagy.

Amsilarotene (TAC101) blocks RB phosphorylation, boosts 2 CDK inhibitors, halts cell cycle, and reduces thymidylate synthase and cyclin A levels.

Ibuprofen Lysine (Neoprofen) is a non-steroidal anti-inflammatory drug.

Nafamostat mesylate (FUT-175), a synthetic serine protease inhibitor, has been used for the treatment of allergic disorders such as asthma, allergic rhinitis

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CASP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CASP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CASP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CASP7 in the samples is then determined by comparing the OD of the samples to the standard curve.

EMT inhibitor-3 (compound 11i) is an epithelial-mesenchymal transition (EMT) inhibitor that effectively suppresses the proliferation, migration, and invasion of SK-N-SH neuroblastoma cells, with an IC50 value of 2.5 μM. It induces mitochondrial-mediated intrinsic apoptosis in tumor cells by enhancing the Bax/Bcl-2 protein expression ratio, promoting the release of cytochrome C from mitochondria, and activating caspase 9 and caspase 3. EMT inhibitor-3 is applicable for cancer research.