Neuroscience

Berbamine is a natural product extracted from Phellodendron amurense.

FR-188582 is a highly selective cyclooxygenase (COX)-2 inhibitor (IC50: 17 nM).

E2730 is a non-competitive GABA transporter 1 (GAT1) inhibitor with anti-epileptic activity, useful in studying neurological disorders.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NMU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NMU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NMU in the samples is then determined by comparing the OD of the samples to the standard curve.

Iferanserin (S-MPEC) is a selective antagonist of the 5-HT receptor (serotonin receptor) with an affinity for 5-HT2A receptors.

3-Hydroxy agomelatine (3-Hydroxyagomelatine) is a 5-HT2C receptor antagonist used in the study of neurological disorders.

AQW051 (VQW-765) is an oral α7-nAChR activator for cognitive impairments, used in anxiety disorder studies.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CR in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PMP22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PMP22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PMP22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PMP22 in the samples is then determined by comparing the OD of the samples to the standard curve.

Emapalumab (NI-0501), an IgG1 antibody, blocks interferon gamma, curbing inflammation. Used in HLH studies, it binds IFN-γ tightly (Kd=1.4 pM).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NPTX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPTX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NPTX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPTX2 in the samples is then determined by comparing the OD of the samples to the standard curve.

2-Bromo-6-methoxynaphthalene was used in the synthesis of nabumetone [4-(6-methoxy-2-naphthalenyl)-2-butanone] by Heck reaction.

Nifedipine (Procardia) is a dihydropyridine calcium channel blocking agent.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSP90α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSP90α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSP90α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HSP90α in the samples is then determined by comparing the OD of the samples to the standard curve.

SB399885 is a potent, brain-penetrant, oral SR-6 antagonist with 200-fold selectivity for SR-6 receptors.

Applications Dehydro Aripiprazole-d8, is the labeled analogue of Dehydro Aripiprazole (D229150), a metabolite of Aripiprazole, a selective dopamine D2-receptor antagonist with dopamine autoreceptor agonist activity. References Canive, J.M., et al.: Psychopharmacol. Bull., 34, 101 (1998), Oshiro, Y., et al.: J. Med. Chem., 41, 658 (1998),

SB215505: potent 5-HT2B/2C blocker, enhances rat wakefulness/motor activity. (pKi: 5-HT2B - 8.3, 5-HT2C - 7.66, 5-HT2A - 6.77).

DOAM is an antagonist of the 5-HT2 receptor.

Azaphen (Pipofezin hydrochloride) is the reuptake of serotonin inhibitor.with antidepressant action and has sedative effects, suggesting antihistamine activity.

4-Methoxyphenyl isothiocyanate (4-Methylphenyl ITC) is recognized as an antioxidant demonstrating an IC50 of 1.25 mM for scavenging DPPH free radicals. Its antioxidant capacity, as shown by the ORAC test, is 11.7 mM TE, equivalent to the efficacy of 11.7 mmol Trolox under similar conditions. Additionally, it extends the oxidation process in the Briggs-Rauscher reaction by approximately 9180 seconds. The compound also exhibits moderate cholinesterase inhibitory activity, with an inhibition rate of 30.4% for acetylcholinesterase (AChE) and 17.9% for butyrylcholinesterase (BChE). Research suggests 4-Methoxyphenyl isothiocyanate holds promise for applications in antioxidant and neurological disorder studies.

AZD 3043 (THRX 918661) is a modulator of the gamma-aminobutyric acid type A receptor, a novel sedative-hypnotic, and a potential short-acting anesthetic.

Impurity Escitalopram EP Impurity K Stability Hygroscopic Applications (R)-Citalopram Oxalate (Escitalopram EP Impurity K) is an inhibitor of serotonin (5-HT) uptake. Antidepressant. References Christensen, A.V., et al.: Eur. J. Pharmacol., 41, 153 (1977), Keller, M.B., et al.: J. Clin. Psychiatry, 61, 896 (2000), Von Moltke, L.L., et al.: Drug Metab. Dispos., 29, 1102 (2001), Fish, E.W., et al.: J. Pharmacol. Exp. Ther, 308, 474 (2004),

Applications Labelled Ricinine. An alkaloid extract from the seeds of the castor-oil plant (Ricinus communalis). Ricinine is a central nervous system stimulant that could potentially be a cognition-enhancing agent used for the treatment of human amnesias. Ricinine also has bactericidal effects on three pests and heptaprotective activity. Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package References Feraz, A.C. et al.: Pharmacol. Biochem. Behav., 63, 367 (1999); Zhao, Z. et al.: Zhong. Niang., 6, 13 (2009);

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat ENK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ENK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ENK in the samples is then determined by comparing the OD of the samples to the standard curve.

25N-NBOMe hydrochloride is a derivative of 2C-N and acts as an agonist for the 5-HT2A and 5-HT2C receptors, with Ki values of 0.144 nM and 1.06 nM, respectively. It has minimal effect on the release of preloaded neurotransmitters from recombinant dopamine, serotonin, and norepinephrine transporters.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SNCa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SNCa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SNCa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SNCa in the samples is then determined by comparing the OD of the samples to the standard curve.

(-)-Dizocilpine maleate ((-)-MK 801 (Maleate)) is a potent, selective and non-competitive NMDA receptor antagonist with Kd of 37.2 nM in rat brain membranes.

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken CCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CCK in the samples is then determined by comparing the OD of the samples to the standard curve.

CDD0102 HCl is a selective and potent M1 muscarinic receptor agonist for the treatment of Alzheimer's disease.

Venlafaxine HCl, a cyclohexanol phenethylamine, acts as an SNRI antidepressant.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EAAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EAAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EAAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EAAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DAT in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP90α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP90α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP90α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSP90α in the samples is then determined by comparing the OD of the samples to the standard curve.

Adatanserin hydrochloride (WY50324 hydrochloride) is a 5-HT(1A)/5-HT(2) receptor ligand that is neuroprotective and may be used in the study of depression.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Thy1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Thy1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Thy1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Thy1 in the samples is then determined by comparing the OD of the samples to the standard curve.

CIA-1 inhibits the nuclear receptor COUP-TFII, with IC50s ranging from 1.2 μM to 7.6 μM in prostate cancer cell lines.CIA-1 inhibits the growth of a variety of

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAST in the samples is then determined by comparing the OD of the samples to the standard curve.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Aβ1-40. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human Aβ1-40. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human Aβ1-40. You can calculate the concentration of Human Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

TP003 is a novel non-selective GABAA receptor benzodiazepine site agonist with affinity for α1β2gam2, α2β3gam2, α3β3gam2, α5β2gam2, EC50 of 20.3, 10.6, 3.24, 5.

Perospirone hydrochloride (Perospirone HCl) is an atypical antipsychotic agent for the treatment of schizophrenia.

A-484954 (A 484954) is a highly specific eukaryotic elongation factor-2 (eEF2, IC50: 280 nM) inhibitor.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CR in the samples is then determined by comparing the OD of the samples to the standard curve.

Desformylflustrabromine hydrochloride (dFBr hydrochloride) is a selective agonist of nicotinic acetylcholine receptor (nAChR) in α4β2 neurons, with pEC50 of 6.

Anti-LRRK2 Antibody (1C773) is an antibody targeting LRRK2. Anti-LRRK2 Antibody (1C773) can be used in ELISA, IHC.

RPR104632 is a novel and potent antagonist located at the NMDA glycine site with neuroprotective activity.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CGRP1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse CGRP1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse CGRP1. You can calculate the concentration of Mouse CGRP1 in the samples by comparing the OD of the samples to the standard curve.

RU 24969 (5-methoxy-3-(1,2,3,6-tetrahydropyridin-4-yl)-1H-indole) is a selective agonist of 5-HT1A and 5-HT1B receptors.

Ipratropium bromide, safer than atropine, treats bronchial disorders, rhinitis, and irregular heartbeat via inhalation.

MRZ 2-514 (8-bromo-4,5-dihydroxypyridazino[4,5-b]quinolin-1-one) is a strychnine-insensitive modulatory site of the NMDA receptor (glycineB)antagonist with Ki

FR 122047 (1-[(4,5-Bis(4-methoxyphenyl-2-thiazoyl)carbonyl]-4-methylpiperazine, Hydrochloride) is a potent and selective inhibitor of COX-1 with IC50 of 28 nM

Suriclone (RP 31264), a cyclic pyrrolidone for anxiety, modulates GABA-A receptors without major sedation.

Lirequinil (Ro 41-3696) is a small molecule GABAA receptor agonist used to study neurological disorders.

SSR180711 hydrochloride (SSR-180711A HCl) is an Alpha7 nicotinic acetylcholine partial agonist that can be used to study neurolysis.

Rapastinel (TPPT-amide) (GLYX-13) is an N-methyl-D-aspartate receptor (NMDAR) modulator.

6-fluoro-DL-Tryptophan is an inhibitor of serotonin (5-HT) synthesis.

Anti-Phospho-PAK1/PAK2/PAK3 (Ser144/Ser141/Ser139) Antibody (6I203) is an antibody targeting Phospho-PAK1/PAK2/PAK3 (Ser144/Ser141/Ser139). Anti-Phospho-PAK1/PAK2/PAK3 (Ser144/Ser141/Ser139) Antibody (6I203) can be used in ELISA, WB, IHC.

TG6-10-1 is an EP2 antagonist.

Glycine (2-Aminoacetic acid) is a non-essential amino acid and a fast inhibitory neurotransmitter, found in gelatin and silk fibroin and used as a nutrient.

2-(Phosphonooxy)benzoic acid (Fosfosal), used as the anti-inflammatory agent, has the anti-bacterial effect.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CHRM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHRM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CHRM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CHRM2 in the samples is then determined by comparing the OD of the samples to the standard curve.

gamma-DGG acetate(6729-55-1 free base) (γ-D-Glutamylglycine) is a competitive blocker of AMPA receptor.

Epinastine hydrochloride (WAL-801CL HCl) is an antihistamine and mast cell stabilizer that is used in eye drops to treat allergic conjunctivitis.

Anti-SNAP23 Antibody (8Y494) is an antibody targeting SNAP23. Anti-SNAP23 Antibody (8Y494) can be used in ELISA, IHC.

9-Cyclopentyladenine (NSC-19486) is an adenylate cyclase inhibitor that blocks cellular morphological differentiation and phosphorylation of specific signaling

"Fenlean (FLZ) in phase I trial at Chinese Academy's Institute of Pharmacy for Parkinson's treatment."

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EAAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EAAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EAAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EAAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

p-Xylene bis(pyridinium bromide) (compound 21) is a cationic quenching agent and acts as a weak bis-quaternary ammonium salt inhibitor for AChE and BChE, with IC50 values of 1540 μM and 529 μM, respectively.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a1ACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a1ACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a1ACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a1ACT in the samples is then determined by comparing the OD of the samples to the standard curve.

Alaproclate is a non-competitive NMDA receptor antagonist and a new selective 5-HT uptake inhibitor for the study of depression and dementia.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human XCL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XCL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human XCL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human XCL2 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AHNAK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AHNAK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AHNAK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AHNAK in the samples is then determined by comparing the OD of the samples to the standard curve.

PTUPB is a potent and dual inhibitor of sEH and COX-2 enzymes(IC50 of 0.9 nM and 1.26 μM, respectively).