Enzyme Substrates

Chromogenic substrate for cellobiohydrolase and endoglucanase.

3-Indoxyl phosphate p-toluidine salt is a secretor of blood group A. It is used in the detection of the presence of alkaline phosphatase in serum and other body fluids. This compound has been shown to be an isoform of 3-indoxyl phosphate with a molecular mass of about 4,000 daltons. It is also used as a substrate for gel electrophoresis and filtration experiments. The purified form of this compound has been shown to have an alkaline phosphatase activity that is ˜4 times greater than that found in human serum and intestinal extracts.

4-Nitrophenyl 2-acetamido-3-O-(2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2,6,8,10,12-pentaoxoheptaacetic acid is a chemiluminescent substrate that is used for the detection of peroxidase and other enzymes. The reaction product is a yellow fluorescent product which can be measured using a fluorometer. It is sold as an aqueous solution at 1mg/mL in phosphate buffer at pH 7.4.

4-Methylumbelliferyl-a-D-mannopyranoside-2-phosphoethanolamine is a fluorescent substrate used in enzyme assays. It is an analog of the natural substrate, 4-methylumbelliferyl-alpha-D-mannopyranoside, which is a phosphoethanolamine linked to alpha-D-mannose. This product can be used as a chromogenic or fluorogenic substrate for various enzymes, including beta glucosidase, beta galactosidase, and beta lactonase. 4MUMPE can also be used for bioluminescent studies and has been shown to have high purity and quality.

Naphthol AS-D is a diagnostic chemical for phosphatase enzymes and is used to stain cells, tissues, and body fluids for diagnosis. The chemical reacts with the amino groups of proteins in cells and produces a brown color. Naphthol AS-D is not toxic to living cells in concentrations below 5%. It stains basophilic leukemia cells as well as brain cells. This chemical can be used to diagnose cutaneous lesions, such as psoriasis or melanoma, which are difficult to diagnose by standard methods.  present in these lesions and produce a brown color.

Aldol® 515 palmitate is a chromogenic substrate that is used in food testing, diagnostics, and other applications. It has a high purity and is conjugated to various ligands for specific applications. Aldol® 515 palmitate is an excellent chemiluminescent substrate for the detection of ATP and other nucleotides. It also has high bioluminescence activity for the detection of adenosine-5'-triphosphate (ATP) or luciferin as well as enzyme substrates to measure β-galactosidase or β-glucuronidase activity.

5-Bromo-3-indoxyl-beta-D-galactopyranoside-6-sulfate sodium salt is an enzyme substrate that has been used in diagnostic tests for detecting the presence of alpha galactosidase. 5-Bromo-3-indoxylbDgalactopyranoside 6 sulfate sodium salt is a ligand that has been shown to bind to the receptor on macrophages and neutrophils, which are cells that protect the body from infection. 5Bromo3indoxylbDgalactopyranoside 6 sulfate sodium salt can be used as a chromogenic substrate or fluorogenic substrate for chemiluminescence reactions. It can also be used in culture media for bacterial growth and for food testing.

Coelenterazine e is a synthetic, fluorescent amino acid that can be used to measure calcium ion concentration. It binds to calcium ions and emits light when excited with blue light. Coelenterazine e is an analog of coelenteramide, which is a luminescent protein found in jellyfish. Coelenterazine e has been shown to emit fluorescence when it interacts with calcium ions, while coelenteramide emits bioluminescence instead. The emission of light from coelenterazine e is due to the stabilization of the molecule by calcium ions.

Used for synthesis of chiral carboxylic acids from nitriles

6,8-Difluoro-4-methylumbelliferyl b-D-galactopyranoside, commonly known as DiFMUG, is a fluorogenic substrate to detect beta-galactosidase. It has higher sensitivity than the common MUG (4-methylumbelliferyl beta-D-galactopyranoside) subtrate.

Aldol® 518 acetate is a fluorogenic substrate for the enzyme aldolase. It is used in diagnostic kits for detecting aldolase in blood and other body fluids, and as an indicator of liver function. Aldol® 518 acetate can be used to detect the presence of aldolase in foods and beverages. The compound has also been shown to be an excellent substrate for bioluminescence and chemiluminescence reactions, which are widely used in research laboratories.

A fluorogenic MOSCERDAM® substrate targeting cellulases

Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.

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Luciferin-caprylate is a chemiluminescent substrate that is used in the detection of various substances. It is a high purity compound with CAS No. 583-22-8 and an E number of E711. This product can be used in diagnostic, food testing, and other applications. Luciferin-caprylate has been shown to be an excellent substrate for bioluminescence, fluorescence, and chemiluminescence reactions. It generates light through the oxidation of luciferin by the enzyme luciferase in the presence of oxygen or other oxidizing agents. The light generated by this reaction can be detected using a photomultiplier tube or luminometer, which can then be used to detect substances such as bacteria, protozoa, or fungi.

Chromogenic substrate for ?-glucosidase

6-Bromo-2-naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.