Enzyme Substrates

Modified naphtol AS substrate used in histochemical and cytochemical staining

Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.

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Fluorogenic substrate used to differentiate cytochrome P450 monooxygenases

Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.

L-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.

Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

Bis(4-methylumbelliferyl)phosphate is a fluorescent probe that can be used for the diagnosis of β-glucosidase. It has a phosphate group and can be used to study phosphodiesterases and phosphodiesters. Bis(4-methylumbelliferyl)phosphate is also a liposomal probe that can be used in kinetic studies. This compound has been shown to inhibit β-glucosidase, which is an enzyme found in human saliva and pancreatic juice, at high concentrations. The inhibition of this enzyme leads to the accumulation of glucose and galactose in the blood, which can lead to diabetes mellitus or cancer.

L-Ala-PAB-resorufin is a highly sensitive chromogenic and fluorogenic indicator for L-alanine aminopeptidase activity and related enzymes occurring in prokaryotic as well as eukaryotic organisms. After removal of the L-alanyl substructure by enzymatic hydrolysis and spontaneous elimination of the para-aminobenzyl spacer, the initially orange colored compound yields an intense pink color and red fluorescence.

Use as a positive control when detecting nitroreductase activity

Aldol® 470 L-alanine ester, hydrochloride is a fluorescent chemiluminescent substrate. It is a ligand with high purity and high quality, which has been patented in the European Patent Office (EP 2427431) and the United States Patent and Trademark Office (US 8940909). The product can be used for chemiluminescence, staining, fluorescence, bioluminescence, enzyme substrate and food testing. Aldol® 470 L-alanine ester, hydrochloride is an excellent fluorescent chemiluminescent substrate that can be used for conjugation to enzymes or antibodies.

2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

4-Nitrophenyl phosphate potassium salt is a high quality, non-radioactive chemiluminescence substrate that is used in the detection of oxidase activity. This product can be used to measure the activity of peroxidase and catalase enzymes, as well as the accumulation of hydrogen peroxide. It also has been shown to be a ligand for some sulfhydryl groups and an inhibitor of phosphatases. 4-Nitrophenyl phosphate potassium salt has been tested for its ability to inhibit bacterial growth and fluorescence by staining cells. This product has also been shown to have bioluminescent properties and can be used for diagnostics or culture media.

5-Bromo-3-indolyl nonanoate (BIN) is a chromogenic enzyme substrate often used for the detection of esterase activity. It is hydrolyzed by esterase to produce a blue product, which can be detected visually or by spectrophotometry. BIN is particularly useful for the detection of esterase activity in bacteria and fungi, and can be used to differentiate between different types of organisms based on their esterase activity patterns

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.

4-Methylumbelliferyl b-D-fucopyranoside is a fluorescent compound that can be used to measure photosynthetic activity. It is often used as a marker for the study of photosynthesis. This compound is produced by organisms in the process of photosynthesis and can be detected in the light emitted by plant leaves when they are exposed to light. 4-Methylumbelliferyl b-D-fucopyranoside is also an important component of biomass and can be used to determine biomass in aquatic environments. The production of this compound may be encoded by genes that regulate its synthesis, and it may also contain polynucleotides.