Apoptosis

LYN-1604 is a novel ULK1 activator.It inducing cell death involved in ATF3, RAD21, and caspase3, accompanied by autophagy and apoptosis.

Applications Labelled Tamoxifen (T006000). A nonsteroidal estrogen antagonist of interest in the treatment of some forms of breast cancer. Tamoxifen is a protein kinase C inhibitor, and induces apoptosis in human malignant glioma cell lines. References Sutherland, R., et al.: Nature, 267, 434 (1977), Lerner, L., et al.: Cancer Res., 50, 4177 (1990), Love, R., et al.: J. Clin. Oncol., 20 2559 (2002),

Niraparib hydrochloride (MK-4827) is a PARP inhibitor with potential cancer treatment effects, causing DNA damage and apoptosis.

Bromosporine is a broad spectrum inhibitor for bromodomains for BRD2/4/9 and CECR2 (IC50: 0.41/0.29/0.122/0.017 μM), respectively.

Obovatol has antioxidant, neuroprotective, antiinflammatory, antithrombotic and antitumour effects, it is a potent NF-κB inhibitors for Alzheimer's disease

AM-8735 is an inhibitor of MDM2 ( IC50: 25 nM).

ACBI1 is a potent PROTAC degrader of BAF ATPase subunits SMARCA2 and SMARCA4, also degrades the polybromo-associated BAF (PBAF) complex member PBRM1, with DC50s

Applications 6-Methyl-hepten-2-one is used in the synthesis of thyrsiferyl 23-Acetate which acts as an anti-leukemic inducer of apoptosis. References Gonzalez, I. et al.: J. Am. Chem. Soc., 122, 9099 (2000);

Niraparib (MK-4827), a PARP inhibitor, boosts DNA breaks to trigger genomic instability and apoptosis, offering anti-cancer effects.

IC 86621: DNA-PK ATP-competitive inhibitor, IC50 120 nM, enhances DSB antitumor effects, EC50 68 µM for repair.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRAIL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRAIL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRAIL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRAIL in the samples is then determined by comparing the OD of the samples to the standard curve.

SACLAC is a cysteine asparaginase activation inhibitor with antitumor activity used in the study of acute myeloid leukemia and cancer.

Applications FK866 is a highly specific noncompetitive inhibitor of nicotinamide phosphoribosyltransferase (NMPRTase), that induces tumor cell apoptosis, and thus is a potential anticancer drug. References Hasmann, M., Schemainda, I.: Cancer Res., 63, 7436 (2003); Khan, J., et a.: Nat. Struct. Mol. Biol., 13, 582 (2006);

Propylparaben (Propyl parahydroxybenzoate) is an antimicrobial agent, preservative, flavouring agent

Kinsenoside ((+)-Kinsenoside) shows significant antihepatotoxic, and anti-inflammatory activities.

ZLDI-8 is an inhibitor of Notch activating/cleaving enzyme ADAM-17 and inhibits the cleavage of Notch protein.

Anticancer Agent 110 exhibits potent in vitro anti-leukemia activity, demonstrating high cytotoxicity against K-562 lineage chronic myelogenous leukemia cells

Prinaberel (ERB-041) is an effective and selective ERβ agonist and >200-fold selective for ERβ.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PYCARD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PYCARD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PYCARD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PYCARD in the samples is then determined by comparing the OD of the samples to the standard curve.