Enzymes dans les Protéines Recombinantes

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Glutaminyl cyclase, also known as QPCT, can promote the N-terminal cyclization reaction of N-terminal pyroglutamate(pGlu).

SUCLA2 Protein, Human, Recombinant (His & Myc) is expressed in E. coli with N-10xHis, C-Myc. The accession number is Q9P2R7.

TrkA Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Muscle, skeletal receptor tyrosine-protein kinase, also known as Muscle-specific tyrosine-protein kinase receptor, Muscle-specific kinase receptor, and MUSK, is

Phospholipase D 040, concentrated (100 U/mg), spray-dried, recombinant, of prokaryotic origin, is an enzyme preparation designed for advanced biochemical applications. This enzyme is derived from a prokaryotic source through recombinant DNA technology, allowing for high purity and activity. Phospholipase D functions by catalyzing the hydrolysis of phospholipids to produce phosphatidic acid and free head groups, which is a crucial reaction in both lipid signaling and membrane dynamics. Its robust enzymatic activity and defined kinetic properties make it an essential tool for researchers studying cell signaling, membrane composition, and lipid metabolism. The spray-dried form ensures stability and ease of use in various experimental setups, from in vitro assays to more complex in vivo systems. Recognized for its significance in biochemical pathways, Phospholipase D 040 plays a critical role in elucidating mechanisms of cellular communication and function. Its applications extend to the development of novel therapeutic approaches targeting lipid-associated disorders and contribute to advancements in molecular biology and biochemistry research.

Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag.

D-Ribulose 1,5-diphosphate carboxylase, commonly known as RuBisCO, is an essential enzyme that catalyzes the first major step of carbon fixation, a process by which inorganic carbon from the atmosphere is converted into organic molecules. This enzyme is derived from spinach, a common model organism used in plant biology research due to its accessibility and well-characterized photosynthetic pathways. RuBisCO operates by catalyzing the reaction of carbon dioxide with ribulose-1,5-bisphosphate (RuBP) to form two molecules of 3-phosphoglycerate. This reaction constitutes a crucial part of the Calvin cycle, which takes place in the chloroplasts of plant cells. As the most abundant protein on Earth, RuBisCO plays a critical role in the conversion of carbon dioxide into organic compounds, ultimately supporting the food chain through its foundational role in photosynthesis. Given its pivotal role, RuBisCO is of great interest in studies involving photosynthetic efficiency, plant biochemistry, and strategies to enhance crop yield. Researchers often explore its structural properties, kinetics, and potential for genetic engineering to improve its catalytic efficiency to meet global food demands and address climate change challenges.

Protocatechuate 3,4-dioxygenase is a bacterial enzyme, which is sourced from Pseudomonas sp. This enzyme operates by catalyzing the cleavage of aromatic rings in protocatechuate, a derivative of catechol. Its mode of action involves the incorporation of oxygen into protocatechuate, resulting in the formation of beta-carboxy-cis,cis-muconate. This reaction is crucial for the microbial degradation of aromatic compounds, thereby playing a significant role in the biodegradation pathways of lignin-derived aromatic pollutants. Widely studied for its significance in environmental bioremediation, protocatechuate 3,4-dioxygenase is key to breaking down complex organic materials in soil and water ecosystems. Its applications extend to industrial processes where the enzymatic transformation of aromatic compounds is required, such as in the synthesis of bio-based chemicals. Researchers also investigate its potential in metabolic engineering to enhance microbial strains for efficient degradation of environmental pollutants. Understanding and utilizing this enzyme can significantly contribute to advances in biotechnology and ecological sustainability.

The p38 mitogen-activated protein kinase (MAPK) pathway plays an important role in cellular responses to inflammatory stimuli and environmental stress.

ACYP1, also known as Acylphosphatase-1, Acylphosphatase, erythrocyte isozyme, Acylphosphatase, organ-common type isozyme, Acylphosphate phosphohydrolase 1 and

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp)

GUCY2C Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag.

Factor XI (FXI) is the zymogen of a plasma protease (FXIa) that contributes to hemostasis by activating factor IX (FIX).

Has pectate lyase activity.

VEGFR3/FLT4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal

SMAD Family Member 1 (SMAD1) is a member of the dwarfin/SMAD family.

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin.

N/A.

Carbonic Anhydrase 1 Protein, Human, Recombinant (Active, His) is expressed in E. coli with C-6xHis. The accession number is P00915.

ABCB1 Protein, Human, Recombinant (His) is expressed in E. coli.

IL-1R1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Kallikrein-4, also known as Enamel matrix serine proteinase 1, Kallikrein-like protein 1, KLK-L1, Serine protease 17, KLK4, PRSS17, and EMSP1, is a secreted

Coagulation Factor II Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

RAF1 Protein, Human, Recombinant (His & SUMO) is expressed in E.

Ribonuclease T1 is an endonuclease enzyme, which is derived from the fungus Aspergillus oryzae. It specifically cleaves single-stranded RNA at the 3' end of guanosine residues, which involves hydrolyzing the phosphodiester bond to produce 3′-phosphomononucleotides and 5′-hydroxylated oligonucleotides. This enzyme’s high specificity and catalytic efficiency make it valuable for various applications. Ribonuclease T1 is widely used in molecular biology for RNA sequencing, structural studies, and RNA mapping. It facilitates the investigation of RNA secondary structures and has been instrumental in determining the primary structure of numerous RNAs. Its specificity for guanosine residues makes it an ideal tool for studying RNA sequences with a known or hypothesized abundance of guanine. In enzymology and structural studies, Ribonuclease T1 helps decipher the role of RNA in protein synthesis and cellular regulation. Additionally, it contributes to advancements in biotechnological applications where RNA processing and manipulation are critical. Such applications highlight the enzyme’s importance in both basic research and applied sciences.

Ricin Protein, Ricinus communis, Recombinant (His) is expressed in E.

Carboxypeptidase B1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

SDS Protein, Human, Recombinant (His & Myc) is expressed in E. coli.

MRAS Protein, Human, Recombinant (His & Myc) is expressed in E. coli with N-10xHis, C-Myc. The accession number is O14807.

Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan

ErbB4 Protein, Human, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

Hydrolyzes carbapenems, penicillins, cephalosporins and aztreonam with varying efficiency.

Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications.

BACE2, also known as beta secretase 2, belongs to the peptidase A1 family.

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in

SMARCA4 Protein, Human, Recombinant (His) is expressed in E.

Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.

Kallikrein-2 (KLK2) is a secreted serine protease that belongs to the peptidase S1 family of Kallikrein subfamily.

Heat-Responsive Protein 12 (HRSP12) is an endoribonuclease that belongs to the Rut family.

Phosphomannomutase 1 (PMM1) blongs to the eukaryotic PMM family.

Iron-storage protein.

RAN Protein, Human, Recombinant (GST) is expressed in E.

Carbonic Anhydrase 12 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Acetyl-CoA carboxylase Protein, Candida albicans, Recombinant (His) is expressed in E.

Peptidyl-Prolyl Cis-Trans Isomerase D (PPID) belongs to the cyclophilin-type PPIase family and PPIase D subfamily.

PAK1 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.

Sulfatase from Helix pomatia is a highly potent enzyme that is capable of hydrolyzing sulfated compounds and sulfate esters. It has been widely used in various applications such as glucosinolate analysis, genistein extraction preparation, and regiospecificity studies. With a concentration of ≥10,000 units per gram of solid, this sulfatase offers exceptional enzymatic activity for sulfatase assays. It effectively catalyzes the hydrolysis of sulfated substrates, including p-nitrocatechol sulfate, naphthyl sulfate and phenyl sulfates.The enzyme can be incubated with the desired sample to facilitate the release of sulfate groups from sulfated compounds. Sulfatase from Helix pomatia is a valuable tool for researchers and scientists working in diverse fields requiring efficient and reliable enzymatic hydrolysis capabilities. Additionally the enzyme has been found to have industrial applications, such as in the bioconversion of industrial chemicals, where it can be used as a catalyst.  One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

Dihydrofolate reductase (DHFR, 1.5.1.3) is a NADP+/NADPH-dependent oxidoreductase, that reduces dihydrofolate to tetrahydrofolate in the following reaction: dihydrofolate + NADPH + H+ ⇌ tetrahydrofolate + NADP+One unit of dihydrofolate reductase will convert 1.0 μmole of dihydrofolic acid into tetrahydrofolic acid in 1 minute at pH 7.5, 22°C and presence of NADPH.

SRMS Protein, Mouse, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q62270.

Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases,

N/A.

Cymit Quimica’s econoLuciferase™ (econoLuc Cymit Quimica Cat. No. L-8090) is a recombinant luciferase from the firefly Luciola lateralis that has been expressed as a luciferase-GFP fusion protein in E. coli.The luciferase enzyme has been optimized for increased thermo-stability by genetic modification to be stable for one hour at 37°C and up to two days at room temperature.Stabilized econoLuciferase™ thus overcomes the disadvantages and limitations of wild-type Luciferase, notably its short active life, outperforming enzyme from the Photinus pyralis firefly for both performance and stability.This optimized luciferase is the perfect enzyme for ATP detection assays in hygiene control, microbial tests using caged luciferins and it is the luciferase of choice for biochemical tests measuring ATP consumption and production in diverse enzymatic reactions.The product L-8090 is available on a large scale and is intended for use in the chemical, diagnostic, pharmaceutical and related industries.

TCPTP Protein, Mouse, Recombinant (aa 2-314, His) is expressed in Baculovirus insect cells with His tag.

E. coli recombinant α-2,6 sialyltransferase from Neisseria meningitidis. One unit is defined as the amount of enzyme that catalyses the formation of 1 μmol CMP-Neu5Ac from CTP and Neu5Ac per minute at 37 ºC.Activity: 100U/mg

CHST9 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

SULT1A1 Protein, Rat, Recombinant (His & Myc & SUMO) is expressed in E.

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences

Ag85C Protein, Mycobacterium tuberculosis, Recombinant (His) is expressed in E.

Glucose dehydrogenase (GDH) is an enzyme that catalyzes the oxidation of glucose and other sugars. enzyme activity: min 40U/mg

ADAM15 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

MKRN1 Protein, Human, Recombinant (His) is expressed in E. coli with N-10xHis. The accession number is Q9UHC7.

PCSK9 Protein, Human, Recombinant (His & HA & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-8xHis-HA-Avi tag.

Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate

Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-

Candidapepsin-2 Protein, Candida albicans, Recombinant (His) is expressed in E.

FbpB Protein, Mycobacterium kansasii, Recombinant (His & Myc & SUMO) is expressed in E.

CAK1 Protein, S.

Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I

Catalyzes the deacylation of triacylglyceryl and cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and

PCSK9 Protein, Human, Recombinant (D374Y, mFc) is expressed in HEK293 mammalian cells with mFc tag.

Mevalonate kinase belongs to the GHMP kinase family, Mevalonate kinase subfamily.

Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells.

ALKBH5 Protein, Human, Recombinant (His) is expressed in E.

MSRB3 Protein, Human, Recombinant (E. coli, His) is expressed in E. coli. The accession number is Q8IXL7-2.

Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation.

CPA2 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Brain-Specific Serine Protease 4 (BSSP-4) is a serine protease that preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family.

Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications.

Cymit Quimica's Phospholipase D 040 Halal and Kosher Food grade bulk enzyme (EPLD840.6). With one of the most competitive activity rates on the global market and manufactured in Europe, this enzyme is perfect for use in food, diagnostic, therapeutic and nutraceutical industries worldwide.  Phospholipase D is an enzyme that is expressed in almost all types of organisms and whose activity can be harnessed to synthesize critical raw materials, for example phosphatidylserine. Phosphatidylserine has shown itself to be an important functional ingredient in reducing cognitive dysfunction and dementia in the field of nutraceuticals. As a Halal and Kosher food grade enzyme, Cymit Quimica's Phospholipase D is an excellent candidate for a diverse variety of food industry applications. Furthermore our enzyme can be used in diagnostic assays, creating first-class drug delivery systems and APIs.

Leucine aminopeptidase (L-leucine aminopeptidase, Leucyl aminopeptidase, leucyl peptidase, peptidase S; EC 3.4.11.1) is an exopeptidase enzyme. It preferentially catalyses the removal of N-terminal leucine residues from proteins and peptides.

ENGASE Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the

Endoglycosidase H (systematic name glycopeptide-D-mannosyl-N4-(N-acetyl-D-glucosaminyl)2-asparagine 1,4-N-acetyl-β-glucosaminohydrolase, EC 3.2.1.96) is a highly specific enzyme, that cleaves asparagine-linked mannose rich oligosaccharides. One unit of Endoglycosidase H will remove >95% of the carbohydrate from 10μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10μl.The product EEH01.7 is available as a large-scale bulk supply of liquid enzyme solution and is intended for use in the chemical, diagnostic, pharmaceutical and related industries.For removal of all N-linked carbohydrates from proteins see Cymit Quimica's PNGase F enzymes (PNG01.3 - vials for research and PNG01.7 - large-scale bulk supply)

TrkC Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

ELANE Protein, Human, Recombinant is expressed in E. coli with Tag Free. The accession number is P08246.

Is able to cleave peptidoglycan.

Multi-copper oxidase from the family of polyphenol oxidades. It can be also found in many plants and bacteria. Laccase catalyses the oxidation of phenolic compounds such as lignin. It can oxidase non phenolic groups too with the use of mediators, chemical agents involved in the transfer of the electrons during the reaction. In some cases, laccase can also oxidise halogenated compounds. This enzyme is used for enzymatic assays, lignol screening tests and for waste water treatment to remove biodegrdable pollutants.

Plays an important role in fat metabolism.

Glutathione S-transferase P (GSTP1) is an enzyme that contains 1 GST C-terminal domain, 1 GST N-terminal domain.

PSMA3 Protein, Human, Recombinant (His) is expressed in E.

Leukotriene A4 Hydrolase Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

Lipase 32 recombinantly expressed in E. coli comes in a spray-dried formulation. It has its pH optimum at 7 and temp. optimum at 50-70°C. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 32 was shown to hydrolyze p-Nitrophenyl esters of butyrate (100 % activity), octanoate (88 %), laurate (11 %), palmitate (0.3 %) and stearate (0.1 %).

PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

TrkB Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.