Enzymes dans les Protéines Recombinantes

Sialidases have been suggested to be pathogenic factors in microbial infections.

Dysregulation of MST1/STK4, a key kinase component of the Hippo-YAP pathway, is linked to the etiology of many cancers with poor prognosis.

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme

Granzyme A/GZMA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2.

Peroxiredoxin 1 Protein, Mouse, Recombinant (His) is expressed in E.

PPIases accelerate the folding of proteins.

3-Hydroxybutyrate Dehydrogenase Type 2 belongs to the short-chain dehydrogenases/reductases (SDR) family.

N/A.

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Trans-2-Enoyl-CoA Reductase Mitochondrial (MECR) belongs to the zinc-containing alcohol dehydrogenase family.

Lipase 14 recombinantly expressed in E. coli comes in a spray-dried formulation. It has its pH optimum at 6-8 and temp. optimum at 30-40°C. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 14 was shown to hydrolyze p-Nitrophenyl esters of butyrate (74 % activity compared to octanoate), octanoate (100 %), laurate (8 %), palmitate (5 %), stearate (4 %), arachidate (1 %) and behenate (0.6 %).

Lipase 64 recombinantly expressed in E. coli comes in a spray-dried formulation. It has its pH optimum at 7 and temp. optimum at 40-50°C. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 64 was shown to hydrolyze p-Nitrophenyl esters of butyrate (100 % activity), octanoate (0.5 %) and laurate (0.1 %).

Metalloprotease that may play a role in the degradation of COMP.

Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications.

Ubiquitin-Conjugating Enzyme E2 G2 (UBE2G2) is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation, which belong to

GSTP1 Protein, Cricetulus longicaudatus, Recombinant (His) is expressed in Yeast.

Glucokinase belongs to the bacterial glucokinase family.

β-1,4-Galactosyltransferase 1 is an enzyme that catalyzes the synthesis of the glycosaminoglycan-protein linkage in proteoglycans.

Invertase is an enzyme that catalyzes the hydrolysis of sucrose to glucose and fructose and can be found in plants and microorganisms

This product shows beta-lactamase activity against clinically relevant beta-lactam antibiotics such as penicillins, carbapenems and cephalosporins. This product may be of particular interest for laboratory teams who require the sterility testing of biological specimens or for environmental monitoring applications.

Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor

TYK2 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with N-10xHis tag.

DDX3X Protein, Human, Recombinant (B2M & His) is expressed in E. coli.

Carbohydrate sulfotransferase 15, also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, GalNAc4S-6ST, B-cell RAG-associated gene protein, CHST15

May be involved in the digestion of phagocytosed particles in the lysosome, participation in an inflammatory protease cascade, and trimming of peptides for

Dual specificity kinase.

PSMA Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

TrkA Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P04629.

Catalyzes the biosynthesis of agmatine from arginine.

MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family.

Ribonuclease 4 Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2.

CYP11B2 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.

Serine protease that cleaves specifically after the sequence Trp-Glu-Leu-Gln.

ITK Protein, Mouse, Recombinant (aa 351-619, His & GST) is expressed in Baculovirus insect cells with His and GST tag.

PLA2G2D Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

GUCY2C Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Functions as a dosage-dependent inducer in mitotic control.

Mer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag.

Initiates complex N-linked carbohydrate formation.

Carboxypeptidase Q (Cpq) is a member of the peptidase M28 family.

GGT1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

PTPRZ1 Protein, Human, Recombinant (E. coli, His) is expressed in E. coli.

Acts as an intracellular estrogen-binding protein.

PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily.

Glucosyltransferase206-freeze dried is an enzymatic preparation designed for specific biochemical applications. It is derived from microbial sources, where it is produced and purified through advanced biotechnological processes. The enzyme functions by catalyzing the transfer of glucosyl units from donor molecules to specific acceptors, thereby forming glycosidic bonds. This mode of action is crucial in various biosynthetic pathways, particularly in the production of polysaccharides and structural carbohydrates. The utility of Glucosyltransferase206-freeze dried spans multiple scientific fields, including biotechnology, pharmaceuticals, and food chemistry. It is widely employed in the synthesis of oligosaccharides and glycoconjugates, which are essential in the development of therapeutic agents, functional foods, and bio-based materials. Researchers utilize this enzyme to study carbohydrate metabolism and to engineer glucoside derivatives with tailored properties. Its robust performance in catalyzing specific reactions makes it a valuable tool for scientists exploring innovative applications in glycoscience.

GPD1 (Glycerol-3-Phosphate Dehydrogenase 1) is a Protein Coding gene.

Has serine protease-like properties and binds to the skin protein profilaggrin.

Catalyzes the deacylation of triacylglyceryl and cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and

PCSK9 Protein, Human, Recombinant (D374Y, mFc), Biotinylated is expressed in HEK293 mammalian cells with mFc tag.

ADCY3 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is O60266.

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2.

This enzyme hydrolyzes cefotaxime, ceftazidime and other broad spectrum cephalosporins.

GUCY2C Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

TRET Protein, Mouse, Recombinant (His & SUMO) is expressed in E.

Clavin has the same substrate specificity as alpha-sarcin.

PPIB is identified as a candidate gene for OI-IX.

Chloroperoxidase (also known as chloride peroxidase, systemic name chloride:hydrogen-peroxide oxidoreductase, EC 1.11.1.10) is an enzyme that catalyzes chlorination of organic compounds. Overall reaction is the following:R-H + Cl− + H2O2 + H+ → R-Cl + 2 H2O; reaction intermediate is hypochlorous acid (HOCl). One unit of chloroperoxidase will convert 1.0 μmole of substrate per minute.

Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

PFKP plays a critical role in cell proliferation in breast cancer cells.

Cytochrome P450 BM-3 Protein, Bacillus megaterium, Recombinant (His & Myc) is expressed in E. coli.

PSMB6 subunit belongs to the 20S proteasomal subunit family, which plays an importance role in the antigen presentation by MHC class I molecular.

p38 delta/MAPK13 Protein, Human, Recombinant (GST), Activated in vitro is expressed in Baculovirus insect cells with GST tag.

Proinflammatory cytokines decreased the expression of CTSH in human islets and primary rat beta-cells, and overexpression of CTSH protected insulin-secreting

CGTases Protein, Paenibacillus macerans, Recombinant (His) is expressed in yeast with N-6xHis tag.

Enterokinase is a member of the trypsin family of serine proteases.

ATP4A Protein, Human, Recombinant (His & Myc) is expressed in E.

Aminopeptidase I is a specialized proteolytic enzyme derived from the actinobacterium Streptomyces griseus. This enzyme functions by catalyzing the cleavage of amino acids from the N-terminus of peptides, which plays a pivotal role in protein metabolism and regulation. The source of this enzyme, Streptomyces griseus, is well-regarded for producing a variety of bioactive compounds owing to its rich genetic and biochemical repertoire. The mode of action of Aminopeptidase I involves the hydrolysis of peptide bonds, facilitated by its coordination with essential metal ions required for activity. This enzymatic specificity and efficiency make it an invaluable tool in both basic and applied biochemical research. Aminopeptidase I is widely utilized in laboratory settings for protein sequencing and structural studies, owing to its precise cleavage ability. Additionally, it has applications in therapeutic protein processing and the synthesis of peptide-based pharmaceuticals. Its utilization extends to various industrial processes as well, including the production of flavor enhancers and bioactive peptides in the food industry. Overall, Aminopeptidase I from Streptomyces griseus is a critical component in advancing our understanding of protein dynamics and fostering innovation in biotechnology.

PIN1 Protein, Human, Recombinant (His) is expressed in E.

HGD Protein, Human, Recombinant (His) is expressed in E.

Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.

Catalyzes the attachment of pyrrolysine to tRNA(Pyl).

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

Luciferase enzymes sourced from Vibrio fischeri

Tryptases are serine proteases with trypsin-like specificity.

Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma

MKNK1 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q9BUB5.

Lipase CR 03 is an industrial-grade enzyme product that is derived from microbial sources, specifically engineered for robust and efficient catalytic activity. The enzyme is extracted utilizing advanced fermentation techniques that capitalize on the prolific nature of specific strains known for lipase production. Its mode of action involves the hydrolysis of ester bonds in triglycerides, converting them into free fatty acids and glycerol. This catalytic process is critical in various biochemical pathways and industrial reactions. Lipase CR 03 finds its applications primarily in industries focused on bioconversion processes, including the production of biodiesel, where it aids in the breakdown of fats into usable biofuels. Additionally, it is used in the food industry for flavor enhancement and dairy product modification, as well as in the pharmaceutical sector for the synthesis of specific ester compounds. Its ability to operate under a wide range of pH and temperature conditions enhances its versatility across different substrates and application environments, making it a valuable asset in biochemical and industrial processes.

Myeloperoxidase (MPO) is a hemecontaining enzyme belonging to the XPO subfamily of peroxidases.

N/A.

PTP4A2, also known as PRL2 or PTPCAAX2, is short for Protein tyrosine phosphatase type IVA 2.

Acts upon elastin.

UCHL3 Protein, Rat, Recombinant (His) is expressed in E.

The enzyme has great affinity for glucose and mannose.

Legumain Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

CNOT7 Protein, Human, Recombinant (His) is expressed in E. coli with N-6xHis. The accession number is Q9UIV1.

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/

LacBuster™-S 2000 is an enzyme preparation, specifically a beta-lactamase, which is derived from bacterial sources known for their capacity to degrade beta-lactam antibiotics. Through hydrolytic cleavage of the beta-lactam ring, this enzyme neutralizes the efficacy of beta-lactam antibiotics, thereby rendering them inactive. The primary function of LacBuster™-S 2000 is to hydrolyze the beta-lactam ring found in antibiotics such as penicillins and cephalosporins. By breaking this chemical structure, the enzyme effectively eliminates the antibiotic activity, allowing for studies in environments otherwise sensitive to the presence of beta-lactams. This product is utilized extensively in microbiological and biochemical research labs, particularly in studies requiring the selective inactivation of beta-lactam antibiotics. It aids in the maintenance of bacterial cultures or assays where the presence of beta-lactam antibiotics could interfere with experimental outcomes. LacBuster™-S 2000 is an invaluable tool in studies of antibiotic resistance and bacterial enzyme activity.

ErbB4 Protein, Human, Rhesus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

PTGES3 Protein, Human, Recombinant (His) is expressed in Yeast.

Alpha Amylase (Amylase, α-Amylase, 1,4-α-D-glucan glucanohydrolase, glycogenase, ptyalin; systematic name 4-α-D-glucan glucanohydrolase; EC 3.2.1.1, CAS Number [9000-90-2]) is an enzyme that catalyses hydrolysis of large polysacharides into smaller fragments. Alpha amylase targets alpha bonds of 1→4 glycosidic linkages of poly- and oligosaccharides with three or more D-glucose units. One unit of Alpha Amylase will catalyze the hydrolysis of 1.0 μmol of 2-chloro-4-nitrophenyl-α-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C. Human salivary Alpha Amylase is supplied as clear, colorless liquid solution at ≥2000U/mL, specific activity ≥200 U/mg protein. Store at -20°C on arrival.

Glucosyltransferase (GTase) is the enzyme that transfers glucose to another organic molecule (aglycon), establishing glycosidic linkage. UDP-glucose dependent GTases are part of the enzyme family of glucosyltransferases, they are versatile tools in glucosylation reactions. Different GTases have different substrate specificities. For the specificity of GT227 Glucosyltransferase, please see Table 1 that lists the available GTases with GT227 highlighted.Color: beigeForm: lyophilisateProtein content: 0.8 mg/mgThe glucosyltransferase was tested in a glucosylation reaction of a preferred substrate. High conversion after up to 24 h reaction time was observed. Not sure which GTase to choose? Consider our Glucosyltransferase kit, which contains all 8 Glucosyltransferases.

HDAC10 Protein, Human, Recombinant (His) is expressed in E. coli with N-6xHis. The accession number is Q969S8.

Pyruvate oxidase is a phosphorylating oxidoreductase which uses FAD as a cofactor to convert pyruvate into acetate and CO2. Pyruvate oxidase (PoxB) is used in metabolism studies in bacteria and in clinical assays for pyruvate, and requires thiamine pyrophosphate (TPP) and magnesium alongside FAD for optimum activity. Activity unit definition: One unit will produce 1.0 μmole of H2O2 per minute during conversion of pyruvate and pyrophosphate to acetylphosphate and CO2 at pH 5.7 and 37 °C.

MDP1, which is short for nesium-dependent phosphatase 1, is a 176 aa.