Enzymes dans les Protéines Recombinantes

Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A), encoded by human DNA repair genes HHR6A, belongs to the ubiquitin-conjugating enzymes (E2

Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

Hydroxyacid oxidase 1, also known as Glycolate oxidase, HAO1, and GOX1, is a member of the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Renin is a highly specific endopeptidase, related to pepsin, whose only known function is to generate angiotensin I from angiotensinogen in the plasma,

UCHL1 Protein, Mouse, Recombinant (His) is expressed in E.

LacBuster™-S 1000 is an enzymatic product derived from microbial sources, specifically engineered for precision and efficiency. It is a beta-lactamase, which catalyzes the hydrolysis of the beta-lactam ring found in beta-lactam antibiotics such as penicillins and cephalosporins, rendering them inactive. The enzyme achieves this by breaking the amide bond within the beta-lactam ring, a critical structural component necessary for antibiotic activity. LacBuster™-S 1000 is utilized in various applications where neutralization of beta-lactam antibiotics is required. Its primary use is in the pharmaceutical industry for the detoxification of waste streams contaminated with beta-lactam substances, thereby preventing the potential impact on environmental microbiota. Additionally, in research settings, it is employed to study antibiotic resistance mechanisms and to control antibiotic effects in microbiological assays. This enzyme must be handled with precision to ensure effective application, given its specific activity and role in deactivating antibiotics.

ATP2A2 Protein, Human, Recombinant (C-His) is expressed in E.

Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications.

ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene.

N/A.

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a

N/A.

Copper-containing enzyme that catalyzes the first step in the synthesis of melanin

β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety.

FKBP11 serve as biomarker and/or therapeutic target for Acute aortic dissection (AAD).

Tyrosine Hydroxylase Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

Carboxypeptidase Y (EC 3.4.16.1) is an exopeptidase enzyme. It hydrolyzes peptide bonds of C-terminal residues and it remains active in the presence of urea at low to moderate concentrations. One unit of the Carboxypeptidase Y will hydrolyze 1.0 μmole of a chromogenic peptide substrate, releasing C-terminal alanine and generating a light-absorbing product. Carboxypeptidase Y has been obtained from yeast S. cerevisiae, has a broad substrate specificity and can therefore be used in sequence analysis of proteins. Carboxypeptidase Y has a temperature optimum in the 20 - 30 °C range and pH optimum between pH 6 and 7.

Peroxiredoxin 2 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

MCAT Protein, S.

This enzyme is closely associated with an endotoxin-sensitive hemolymph coagulation system which may play important roles in both hemostasis and host defense

Hexokinase (EC 2.7.1.1) in an enzyme that catalyses the following reaction:  Glucose + ATP → Glucose-6-phosphate + ADP  One unit of hexokinase will phosphorylate 1.0 µmole of glucose per min at pH 7.6 and 25 °C in the presence of ATP.  The product is a lyophilized powder, ≥30 units/mg protein.

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion

Protein arginine methyltransferase 3, also known as PRMT3, is one of four type I protein arginine methyltransferases (PRMT) that in humans is encoded by the

FAP Protein, Human, Recombinant (aa 27-760, His) is expressed in Baculovirus insect cells with His tag.

Serine/threonine protein kinase required for cell-type-specific transcription and signal transduction in yeast.

2-5A-dependent Rnase Protein, Human, Recombinant (Myc) is expressed in E.

ABHD5 Protein, Human, Recombinant (His & SUMO) is expressed in E.

Enzyme involved in protein translation and catalyzes the aminoacylation reaction

Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein.

NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of

Selenophosphate synthetase 1 (SEPHS1) belongs to the selenophosphate synthase 1 family, Class II subfamily.

MAPK14 Protein, Mouse, Recombinant (His & Myc) is expressed in E.

Glutamate decarboxylase 1, also known as 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform and GAD1, is a member of thegroup II

Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes,

KRAS Protein,Human,Recombinant(G12D & Q61H, His) is expressed in E.

CYP11B1 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is P15538.

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides (Ref.4, PubMed:11109488).

ErbB4 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

Ubiquitin Conjugating enzyme E2C (other names UBE2C, UBCH10, dJ447F3.2, ubiquitin conjugating enzyme E2 C; EC 2.3.2.24) is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis This activity is essential at then end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBE2C bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBE2C is useful for in vitro ubiquitinylation reactions.

HAAO Protein, Rat, Recombinant (His) is expressed in E.

ADH5 Protein, Human, Recombinant (GST) is expressed in E. coli.

Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity. Capable of producing kinin potentiating peptides.

TrkC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His) is expressed in HEK293 mammalian cells with His tag.

Alcohol dehydrogenase (EC 1.1.1.1) is the enzyme that catalyzes interconversion between alcohols and aldehydes or ketones, using NAD+/NADH as a cofactor in the following reaction: CH3CH2OH + NAD+ ⇔ CH3CHO + NADH + H+     One unit of alcohol dehydrogenase will convert 1.0 µmol of ethanol to acetaldehyde per minute at pH 8.8 and 25 °C.

Chk1 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag.

FKBP7 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

E. coli recombinant sialic acid aldolase (EC 4.1.3.3) from Pasteurella multocida.  One unit is defined as the amount of enzyme that catalyses the formation of 1 umol Neu5Ac from ManNAc and Pyruvate per minute at 37 ℃.Activity: 9U/mg

Alkaline Phosphatase (Germ type)/ALPG Protein, Mouse, Recombinant (His) is expressed in HEK293 Cells with C-10xHis. The accession number is P24823.

G-protein coupled receptor kinase 2 (GRK2), also referred as Adrenergic, beta, receptor kinase 1 (ADRBK1), is a ubiquitous member of the G protein-coupled

PARP4 Protein, Human, Recombinant (His) is expressed in E. coli.

Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans.

CHEK2 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag.

ALPP is a membrane protein and exits as a homodimer.

N/A.

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes.

Estrogen sulfotransferase, also known as Sulfotransferase, estrogen-preferring, Sulfotransferase 1E1, SULT1E1 and ST1E1, is a cytoplasm enzyme that belongs to

Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development

Proto-oncogene tyrosine-protein kinase Yes, also known as Proto-oncogene c-Yes, p61-Yes and YES1, is a cytoplasm protein that belongs to the protein kinase

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

IL-1R1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Kallikrein 5/KLK5 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag.

Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. The CalB02 lipase has a temperature optimum in the 40 - 50 °C range and pH optimum between pH 5 and 8.

TREX1 Protein, Human, Recombinant (His & MBP) is expressed in Baculovirus insect cells with N-10xHis-MBP and C-6xHis tag.

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag.

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc).

Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.

Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide.

Cell wall enzyme

Thrombopoietin Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

FKBP25 contains 1 PPIase FKBP-type domain, belongs to the FKBP-type PPIase family.

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing.

Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin.

Possesses quercetin 7-O-glucosyltransferase and 3'-O-glucosyltransferase activities in vitro.

Cathepsin G Protein, Human, Recombinant (His & Myc) is expressed in E. coli with N-10xHis, C-Myc. The accession number is P08311.

UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family.

Aldehyde dehydrogenase (EC 1.2.1.3) is the enzyme that catalyzes oxidation of aldehydes to carboxilic acids, using NAD+ as a cofactor in the following reaction: R-CHO + NAD+ + H2O → R-COOH + NADH + H+    One enzyme unit will oxidize 1.0 μmole of acetaldehyde to acetic acid per min at 25 °C at pH 8.0 in the presence of β-NAD+, potassium and thiols.

Aromatase/CYP19A1 Protein, Human, Recombinant (His) is expressed in E. coli.

ZC3H12A Protein, Mouse, Recombinant (His & Myc) is expressed in E.

RAB10 Protein, Human, Recombinant (His & Myc) is expressed in Baculovirus.

USP46 Protein, Human, Mouse, Recombinant (SUMO) is expressed in Baculovirus insect cells with SUMO tag.

Serine/threonine-protein kinase D3, also known as Protein kinase C nu type, Protein kinase EPK2, PRKD3, EPK2 and PRKCN, is a cytoplasm and membrane protein that

A N-Acetyl Muramidase enzyme that cleaves N-acetylmuramyl-β(1-4)-N-acetylglucosamine linkage in peptidoglycan within the bacterial cell wall.

Peptidylarginine deiminase type4 (PADI4) was firstly identified as a non-MHC RA genetic risk factor.

Involved in the O-glucosylation of monolignols (alcohol monomers of lignin).

Macrophage-stimulating protein (MSP) is a serum protein belonging to the plasminogen-related growth factor family.

Applications 1-Methyl-D-tryptophan is used in biological studies to determine the effect of IDO2 enzyme (indoleamine-(2,3)-dioxygenase) activity and IDO2-mediated arrest of human T cell proliferation. It reverses IDO-mediated immune suppression References Qian, F., et al.: Cancer Immunology Immunothrapy, 61, 2013 (2012)

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP).

Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related

Sortase A Protein, S.

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum.

PLA2G15 Protein, Chinese hamster, Recombinant (His) is expressed in CHO mammalian cells with His tag.

Mcr-1 Protein, E.

Urokinase/uPA Protein, Human, Recombinant (Activated, His) is expressed in HEK293 Cells. The accession number is P00749-1.

PTPN12 Protein, Human, Recombinant (aa 1-355, His & GST) is expressed in Baculovirus insect cells with His and GST tag.