Enzymes dans les Protéines Recombinantes

Dihydrofolate reductase (DHFR, 1.5.1.3) is a NADP+/NADPH-dependent oxidoreductase, that reduces dihydrofolate to tetrahydrofolate in the following reaction: dihydrofolate + NADPH + H+ ⇌ tetrahydrofolate + NADP+One unit of dihydrofolate reductase will convert 1.0 μmole of dihydrofolic acid into tetrahydrofolic acid in 1 minute at pH 7.5, 22°C and presence of NADPH.

SRMS Protein, Mouse, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q62270.

Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases,

N/A.

Cymit Quimica’s econoLuciferase™ (econoLuc Cymit Quimica Cat. No. L-8090) is a recombinant luciferase from the firefly Luciola lateralis that has been expressed as a luciferase-GFP fusion protein in E. coli.The luciferase enzyme has been optimized for increased thermo-stability by genetic modification to be stable for one hour at 37°C and up to two days at room temperature.Stabilized econoLuciferase™ thus overcomes the disadvantages and limitations of wild-type Luciferase, notably its short active life, outperforming enzyme from the Photinus pyralis firefly for both performance and stability.This optimized luciferase is the perfect enzyme for ATP detection assays in hygiene control, microbial tests using caged luciferins and it is the luciferase of choice for biochemical tests measuring ATP consumption and production in diverse enzymatic reactions.The product L-8090 is available on a large scale and is intended for use in the chemical, diagnostic, pharmaceutical and related industries.

TCPTP Protein, Mouse, Recombinant (aa 2-314, His) is expressed in Baculovirus insect cells with His tag.

E. coli recombinant α-2,6 sialyltransferase from Neisseria meningitidis. One unit is defined as the amount of enzyme that catalyses the formation of 1 μmol CMP-Neu5Ac from CTP and Neu5Ac per minute at 37 ºC.Activity: 100U/mg

CHST9 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

SULT1A1 Protein, Rat, Recombinant (His & Myc & SUMO) is expressed in E.

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences

Ag85C Protein, Mycobacterium tuberculosis, Recombinant (His) is expressed in E.

Glucose dehydrogenase (GDH) is an enzyme that catalyzes the oxidation of glucose and other sugars. enzyme activity: min 40U/mg

ADAM15 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

MKRN1 Protein, Human, Recombinant (His) is expressed in E. coli with N-10xHis. The accession number is Q9UHC7.

PCSK9 Protein, Human, Recombinant (His & HA & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-8xHis-HA-Avi tag.

Functions as a pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate

Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-

Candidapepsin-2 Protein, Candida albicans, Recombinant (His) is expressed in E.

FbpB Protein, Mycobacterium kansasii, Recombinant (His & Myc & SUMO) is expressed in E.

CAK1 Protein, S.

Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I

Catalyzes the deacylation of triacylglyceryl and cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and

PCSK9 Protein, Human, Recombinant (D374Y, mFc) is expressed in HEK293 mammalian cells with mFc tag.

Mevalonate kinase belongs to the GHMP kinase family, Mevalonate kinase subfamily.

Sphingosine Kinase 1/SPHK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells.

ALKBH5 Protein, Human, Recombinant (His) is expressed in E.

MSRB3 Protein, Human, Recombinant (E. coli, His) is expressed in E. coli. The accession number is Q8IXL7-2.

Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation.

CPA2 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Brain-Specific Serine Protease 4 (BSSP-4) is a serine protease that preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family.

Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications.

Cymit Quimica's Phospholipase D 040 Halal and Kosher Food grade bulk enzyme (EPLD840.6). With one of the most competitive activity rates on the global market and manufactured in Europe, this enzyme is perfect for use in food, diagnostic, therapeutic and nutraceutical industries worldwide.  Phospholipase D is an enzyme that is expressed in almost all types of organisms and whose activity can be harnessed to synthesize critical raw materials, for example phosphatidylserine. Phosphatidylserine has shown itself to be an important functional ingredient in reducing cognitive dysfunction and dementia in the field of nutraceuticals. As a Halal and Kosher food grade enzyme, Cymit Quimica's Phospholipase D is an excellent candidate for a diverse variety of food industry applications. Furthermore our enzyme can be used in diagnostic assays, creating first-class drug delivery systems and APIs.

Leucine aminopeptidase (L-leucine aminopeptidase, Leucyl aminopeptidase, leucyl peptidase, peptidase S; EC 3.4.11.1) is an exopeptidase enzyme. It preferentially catalyses the removal of N-terminal leucine residues from proteins and peptides.

ENGASE Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the

Endoglycosidase H (systematic name glycopeptide-D-mannosyl-N4-(N-acetyl-D-glucosaminyl)2-asparagine 1,4-N-acetyl-β-glucosaminohydrolase, EC 3.2.1.96) is a highly specific enzyme, that cleaves asparagine-linked mannose rich oligosaccharides. One unit of Endoglycosidase H will remove >95% of the carbohydrate from 10μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10μl.The product EEH01.7 is available as a large-scale bulk supply of liquid enzyme solution and is intended for use in the chemical, diagnostic, pharmaceutical and related industries.For removal of all N-linked carbohydrates from proteins see Cymit Quimica's PNGase F enzymes (PNG01.3 - vials for research and PNG01.7 - large-scale bulk supply)

TrkC Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

ELANE Protein, Human, Recombinant is expressed in E. coli with Tag Free. The accession number is P08246.

Is able to cleave peptidoglycan.

Multi-copper oxidase from the family of polyphenol oxidades. It can be also found in many plants and bacteria. Laccase catalyses the oxidation of phenolic compounds such as lignin. It can oxidase non phenolic groups too with the use of mediators, chemical agents involved in the transfer of the electrons during the reaction. In some cases, laccase can also oxidise halogenated compounds. This enzyme is used for enzymatic assays, lignol screening tests and for waste water treatment to remove biodegrdable pollutants.

Plays an important role in fat metabolism.

Glutathione S-transferase P (GSTP1) is an enzyme that contains 1 GST C-terminal domain, 1 GST N-terminal domain.

PSMA3 Protein, Human, Recombinant (His) is expressed in E.

Leukotriene A4 Hydrolase Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

Lipase 32 recombinantly expressed in E. coli comes in a spray-dried formulation. It has its pH optimum at 7 and temp. optimum at 50-70°C. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 32 was shown to hydrolyze p-Nitrophenyl esters of butyrate (100 % activity), octanoate (88 %), laurate (11 %), palmitate (0.3 %) and stearate (0.1 %).

PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

TrkB Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.