Substratos Cromogénicos

N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride is a fluorogenic substrate that can be used for the detection of β-glucuronidase. It has been shown to have high purity and quality, which makes it an excellent choice for use in food testing and staining.

4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-3,4,6-tri-O-acetyl-b-D-glucopyranosyl)-4,6-di-O-acetyl-2deoxy--aDgalactopyranoside is a substrate for enzymes such as beta galactosidase and alpha amylase. It can be used for the detection of these enzymes in biological samples, including culture media and body fluids. 4NPAG has also been used as a chromogenic substrate to measure the activity of NADH oxidase in neutrophils. 4NPAG is fluorescent in aqueous solution and can be used to detect bioluminescence reactions.

Naphthol AS-BI sulphate potassium salt is a modified naphtol AS substrate used for alkaline and acid phosphatase.

Please enquire for more information about 5-Bromo-3-indoxyl myo-inositol-1-phosphate, ammonium salt including the price, delivery time and more detailed product information at the technical inquiry form on this page

Aldol® 515 nonanoate hydrochloride is a chromogenic and fluorogenic enzyme substrate for detection of lipase activity. The colorless product is cleaved by C9 esterases produced by bacteria in liquid or solid media, yielding a red color and red fluorescence when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 nonanoate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 24-48 h. In the presence of a purified lipase, color and fluorescence develops within minutes to few hours.

Aldol® 484 alpha-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate for ?-glucosidase activity (EC 3.2.1.20, ?-1,4-glucosidase, also known as maltase). The colorless enzyme substrate is cleaved by bacteria producing this enzyme in liquid or solid media, yielding orange color. Green fluorescence is also generated if a suitable matrix is present such as: cellulose acetate filters, polypropylene tubes or microplates and polyethylene vials. Aldol® 484 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.

5-Bromo-6-chloro-3-indolyl caprylate (or magenta caprylate) is a chromogenic substrate which can be used for the detection of esterase enzyme activity from Salmonella and some Klebsiella and Enterobacter. The esterase cleaves the ester bond in the substrate, releasing 5-bromo-6-chloro-1H-indol-3-ol which undergoes oxidative dimerization to form 5,5'-dibromo-6,6'-dicholoro-indigo, a magenta coloured chromophore (λmax 565nm). This product is patent pending, as polymorphs of this product can have different physicochemical properties and can improve solubility and stability. Cymit Quimica is able to product high quality, stable product to detect esterase enzyme activity.

Chromogenic substrate for beta-glucuronidase

Aldol® 518 alpha-D-glucopyranoside is a fluorogenic enzyme substrate used for detection of alpha-D-glucosidase activity. The colorless enzyme substrate is cleaved by alpha-D-glucosidase, generating red fluorescent signal and in some conditions, an orange colour in the visible spectrum can get formed, staining bacterial colonies in faint orange coloration. Aldol® 518 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.

BIOSYNTH Culture Medium for the isolation andidentification of Escherichia coli O157:H7

5-Bromo-4-chloro-3-indolyl myristate is a chromogenic substrate used to detect the specific enzymatic activity of lipase or esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl myristate is used in screening assays for pancreatic lipase deficiency and lipid metabolism studies.

The 4-Nitrophenyl 2,3,4-tri-O-acetyl-6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is a fluorogenic substrate for β-galactosidase. This product has high purity and quality and is an enzyme substrate for β-galactosidase. The 4NPG2,3,4TRA can be used as a diagnostic tool in the diagnosis of bacterial infections. It is also used in the food testing industry and environmental testing industry. This product has CAS No.: 51743-71-1. This product is also a ligand that reacts with the enzyme β-galactosidase to produce light in chemiluminescence reactions. This product can be used for staining or bioluminescence applications.

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4-Fluoro-2-nitrophenyl beta-D-galactopyranoside, a fluorinated analog of ONPG, is a chromogenic enzyme substrate specifically used to detect beta-galactosidase in bacterial media. 4-Fluoro-2-nitrophenyl beta-D-galactopyranoside is used in Lactose intolerance screening, bacterial beta-galactosidase detection (E. coli)

4-Nitrophenyl 6-O-(b-D-galactopyranosyl)-b-D-galactopyranoside is a fluorescent substrate that is used in diagnostics and culture media. It can be used to detect the presence of aminoglycoside antibiotics, such as gentamicin, by using the fluorogenic or chromogenic reaction. 4NP6BG has been shown to bind to the beta subunit of RNA polymerase and inhibit transcription, which can be useful for testing environmental samples for the presence of aminoglycosides. 4NP6BG is a high quality product with a CAS number of 80321-98-8.

For the detection of beta-galactosidase activity

Histochemical substrate for esterase

5-Bromo-6-chloro-1H-indol-3-yl-a-D-mannopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lysosomal storage disorder screening.

Aldol® 518 beta-D-cellobioside is a chromogenic substrate for the detection of glycosidases. Aldol® 518 beta-D-cellobioside reacts with enzymes such as alpha-amylase, beta-galactosidase, and alpha-glucosidase to produce a fluorescent product. This product is detected by spectrophotometer, fluorometer, or luminometer. The fluorescence intensity is proportional to the concentration of the enzyme in the sample. It has been shown that Aldol® 518 beta-D-cellobioside can be used as a substitute for conventional substrates in food testing and diagnostics, such as staining and culture media. It also has applications in environmental testing and enzyme substrate assays.

X-Gal is a chromogenic substrate for β-galactosidase, yielding a blue precipitate. X-Gal is an indicator for coliforms in culture media, as well as for the detection of coliforms (E. coli ) in municipal water supplies and food products. X-Gal can be used in conjunction with the inducer IPTG, which binds and inhibits the lac repressor thus inducing beta;-galactosidase expression. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. Upon hydrolysis, X-Gal yields a localized, insoluble, blue precipitate, making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. 5-Bromo-4-chloro-3-indoxyl-beta;-D-galactopyranoside (X-beta;-D-galactoside, X-Gal) is a chromogenic indicator for beta;-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a blue-green colored precipitate. X-Gal can also be used as a detecting agent for beta;-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gal. The reaction proceeds only in the presence of oxygen, i.e. in aerobic cultures. Well detectable color bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of purified beta;-galactosidase, color develops within minutes to few hours.