Enzymes dans les Protéines Recombinantes

IDO2 belongs to the indoleamine 2,3-dioxygenase family.

Carboxylesterase 3 Protein, Human, Recombinant (His) is expressed in P. pastoris (Yeast) with C-6xHis. The accession number is Q6UWW8.

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during

Glycine N-Methyltransferase (GNMT) is a tetrameric cytosolic protein.

Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as

Alkaline phosphatase Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

A Disintegrin And Metalloproteinase (ADAM) proteases constitute a family of multifunctional, membrane-bound proteins with traditional sheddase functions.

Has no significant hemorrhagic activity, but inactivates serpins by limited proteolysis of their reactive-site loops.

Plasmin, human is a serin protease which present in the blood and is involved in the cleavage of cross-linked fibrin, a process known as fibrinolysis.One unit will produce one micromole of P-Nitroanilide from D-Val-Leu-Lys-P-Nitroanilide per minute at pH 7.5 at 37°C

Cholesterol dehydrogenase (EC 1.1.1.840) is NADP+-dependant oxidoreductase, that catalyses the following reaction:cholesterol + NADP+ + H2O → cholest-4-en-3-one + NADPH + H+This is achieved by oxidizing alcohol hydroxy-group into ketone. One unit of cholesterol dehydrogenase will produce 1.0 μmole of cholest-4-en-3-one per minute at pH 8.5 and 25 °C.

MAPKAPK2 Protein, Mouse, Recombinant (His & Myc) is expressed in E. coli with N-10xHis, C-Myc. The accession number is P49138.

PPT1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

HK2 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

CRELD1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

One unit of aldolase (EC 4.1.2.13) will convert 1.0 µmol of Fructose-1,6-Diphosphate to Dihydroxyacetone phosphate and Glyceraldehyde-3-phosphate per min at 25 °C and pH 7.4. Lyophilized Powder.

PIM2 Protein, Human, Recombinant (His & Myc) is expressed in E. coli with N-10xHis, C-Myc. The accession number is Q9P1W9.

ALDH1A3 Protein, Human, Recombinant (His) is expressed in E.

Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, involved in both the synthesis and degradation of fructose-2,6-

CMPK1 plays a key role in the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells.

Single-stranded DNA-specific cytidine deaminase.

N/A.

Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).

HSPA8 Protein, Human, Recombinant (His) is expressed in Yeast.

CNOT6L Protein, Human, Recombinant (His) is expressed in E. coli with N-10xHis. The accession number is Q96LI5.

Formaldehyde dehydrogenase (EC 1.2.1.46) is an enzyme that catalyzes the following reaction: formaldehyde + NAD+ + H2O ⇌ formate + NADH + H+ One unit of formaldehyde dehydrogenase will convert 1.0 µmole of formaldehyde to formic acid per minute at pH 7.5 and 37°C in the presence of NAD+.NAD+ is available here.

15 Lipoxygenase 2 Protein, Human, Recombinant is expressed in Baculovirus insect cells.

N/A.

Cathepsin E Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Has endoglucanase activity on carboxymethylcellulose (CMC).

PTPN14 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q15678.

Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose.

PFK1, also known as PFKM, is a regulatory glycolytic enzyme.

Lipoyl synthase Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is O43766.

SVSP Protein, Crotalus atrox, Recombinant (His) is expressed in Yeast.

ENPP3 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-10xHis tag.

POLG2 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli with N-6xHis-SUMO. The accession number is Q9UHN1.

PTP1B Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in E. coli. The accession number is P18031.

Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain

Adenylate kinase 7 Protein, Human, Recombinant (His) is expressed in Baculovirus.

Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial

HSD17B14 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/

ATG14 Protein, Human, Recombinant (His & Myc) is expressed in E.

EGLN3 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q9H6Z9.

IL-1R1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

SOD1 belongs to the Cu-Zn superoxide dismutase family.

PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His), Biotinylated is expressed in HEK293 mammalian cells with His tag.

Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient

tPA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

This enzyme is closely associated with an endotoxin-sensitive hemolymph coagulation system which may play important roles in both hemostasis and host defense

RUVBL2 Protein, Human, Recombinant (His & SUMO) is expressed in E.

SIRT3 Protein, Mouse, Recombinant (His & Myc) is expressed in E.

HARS/HisRS Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

RIPK1 Protein, Human, Recombinant (His & Myc) is expressed in E.

Kit of 10 unique, nitrile hydratases recombinantly expressed in E. coli for screening. Nitrile hydratases can be utilized to convert nitriles into their corresponding amides, e.g. to produce acrylamide from acrylonitrile. Additional applications include the removal of nitriles from industrial wastewater. Please note, that the kit enzymes can also be supplied as whole cell biocatalysts in large scale.

Catalyzes the formation of flavonols from dihydroflavonols.

NLRP3 Protein, Mouse, Recombinant (His & SUMO) is expressed in E.

Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin.

ST2/IL-1 RL1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).

AKR1C2 is a member of the aldo/keto reductase superfamily, which consists of more than 4 known enzymes and proteins.

Human Esterase D is a cytoplasmic serine hydrolase that belongs to the esterase D family.

Catalyzes the reverse reaction of octopine dehydrogenation.

Cysteine Dioxygenase Type 1 (CDO1) is a mammalian non-heme iron enzyme that belongs to the cysteine dioxygenase family.

HAAO Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag.

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion.

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.

PRKAA1 Protein, Mouse, Recombinant (E.

E3 ubiquitin ligase.

GPX3 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is P22352.

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Aldehyde dehydrogenase 1 family member A2 (ALDH1A2), also known as retinaldehyde dehydrogenase 2 (RALDH2), belongs to the aldehyde dehydrogenase family which

The main protease Mpro is a key protein in the lifecycle of the SARS-CoV-2 virus. Mpro cleaves the viral polyproteins at the C-terminal end of a glutamine residue in recognition sequences containing Leu-Gln-(Ser, Ala, Gly) motifs (Rut et al, 2020).  As SARS-CoV-2 MPro has no closely related homologues in humans, it represents an attractive drug target (Ullrich and Nitsche, 2020). In summary, the Mpro protease is a chymotrypsin-like cysteine protease, requires homodimerisation for proteolytic activity, cleaves the viral polyproteins in 11 distinct sites, exclusively after a glutamine residue. A fluorogenic substrate for Mpro assays is Ac-Abu-Tle-Leu-Gln-AMC.The protein amount or better its concentration in solution is quantified using either A280 (absorption at 280 nm with its specific absorption coefficient) or using the Bradford assay (uses the dye Coomassie Brilliant Blue). Both of these methods quantify the total amount of protein in a sample, no matter what the oligomerization state is.

6-Phosphogluconic dehydrogenase is an enzyme from yeast, which is a key component of the oxidative phase of the pentose phosphate pathway. It catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate, with the concurrent reduction of NADP+ to NADPH. This enzyme is sourced from yeast, a model organism extensively used in biochemical studies due to its eukaryotic nature and ease of genetic manipulation. The mode of action involves the enzymatic conversion that not only contributes to cellular redox balance by generating NADPH, but also links glycolytic carbohydrate metabolism to biosynthetic processes and antioxidative defense mechanisms. Understanding and investigating this enzyme is critical for studies on metabolic pathways and regulation. Applications of 6-Phosphogluconic dehydrogenase extend to metabolic engineering, where it is employed to enhance NADPH availability for synthetic biology applications. It also serves as a model for studying enzyme kinetics and regulation, providing insights useful for biotechnological advancements and therapeutic research, particularly in areas involving oxidative stress and metabolic disorders.

LasA Protein, Pseudomonas aeruginosa, Recombinant (His & SUMO) is expressed in E.

CANT1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag.

Catalyzes the oxidation of methanol to yield formaldehyde.

ENTPD3 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag.

RIZ1 Protein, Human, Recombinant (GST) is expressed in E.

Abhydrolase domain containing 4 (ABHD4), also known as alpha/beta-hydrolase 4 (ABH4) , or lyso-N-acylphosphatidylethanolamine lipase, which belongs to the ABHD4

Tissue-type plasminogen activator (PLAT) is a protein that secreted into extracellular space.

Exostosin-like 2 (EXTL2) is a member of the exostosin (EXT)-related family which contains five members: EXT1, EXT2, EXTL1, EXTL2, and EXTL3.

Death-associated protein kinase 3, also known as DAP kinase 3, ZIP-kinase, DAPK3 and ZIPK, is a nucleus and cytoplasm protein which belongs to theprotein

Penase (Penicillinase) is an enzyme, which is a type of β-lactamase sourced from various bacterial strains capable of deactivating penicillin. It accomplishes this by targeting the β-lactam ring, a crucial structural component of penicillin antibiotics, and hydrolyzing it, thereby neutralizing the antibiotic effect. This enzymatic action is a defense mechanism employed by certain bacteria to survive in environments saturated with penicillin-based antibiotics. The primary application of Penase is in microbiological and biochemical research where understanding bacterial resistance mechanisms is critical. Scientists often utilize Penase to study resistance patterns in bacteria, evaluate enzyme kinetics, and develop strategies to counteract bacterial defense mechanisms. Additionally, it serves as an important tool in environmental microbiology, assisting researchers in exploring the dissemination of antibiotic resistance genes. The insights gained are invaluable in shaping the development of next-generation antibiotics that are effective against resistant bacterial strains, thus contributing to global healthcare initiatives against antibiotic resistance.

A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis.

In a single vial and in a single step, the X-ShiningTM all-in-one ATP Assay provides an optimized reaction solution for the specific bioluminescent detection of ATP.  Simply reconstitute, and the X-ShiningTM all-in-one ATP Assay solution can be directly applied to samples for ATP measurement in a 1:1 ratio for an immediate bioluminescent read-out. The measurement of ATP levels is crucial to study cell cultures, cell viability, cell response, biochemical processes, to monitor environmental sample activity levels, to assess water quality, to test for biological contamination and to assess biocide efficacy. The X-ShiningTM all-in-one ATP Assay provides sufficient reagent solution to perform 50-to-100 measurements in a 96-well plate and can be easily adapted to measurements in tubes. Find out more about our innovative X-ShiningTM range here X-Shining | Innovation | Cymit Quimica .

Dipeptidyl peptidase 3(DPP3), is a member of the S9B family in clan SC of the serine proteases.

Alkaline Phosphatase/ALPL Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular

Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN).

Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces. Lipases/esterases can be used as versatile tools in hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications.

Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser

Alteplase is human plasminogen activator (EC 3.4.21.68, that cleaves plasminogen into enzymatically active form, plasmin), recombinantly expressed in CHO cells. Alteplase belongs to the group of thrombolytic agents, and it has shown to be effective in restoring blood flow by breaking down clots.

Neuroendocrine convertase 1, also known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is an enzyme which belongs to the peptidase S8

Human Vascular endothelial growth factor receptor 1(VEGFR-1, FLT-1) is a member of the the class III subfamily of receptor tyrosine kinases (RTKs) and Tyr

Probable Xaa-Pro Aminopeptidase 3 (XPNPEP3) is a member of the peptidase M24B family.

GalNAc-transferase-7 (GALNT7) is essential for the regulation of cell proliferation and has been implicated in tumorigenesis.

Can convert androstan-3-alpha,17-beta-diol (3-alpha-diol) to androsterone in vitro, suggesting that it may participate in androgen metabolism during

LRRK2 Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q5S007.

Chymotrypsin C (abbreviated for CTRC), also known as caldecrin or elastase4, is a digestive enzyme of the peptidase S1 family.

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

Mer Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag.

Lipase 17 recombinantly expressed in E. coli comes in a spray-dried formulation. It has its pH optimum at 7-8. Lipases belong to the family of esterases and naturally act on triglycerides at lipid-water interfaces catalyzing hydrolytic reactions, esterifications and transesterification reactions in industrial and food applications. Lipase 17 was shown to hydrolyze p-Nitrophenyl esters of butyrate (2 % activity compared to octanoate), octanoate (100 %), laurate (20 %), palmitate (3 %), stearate (1 %), arachidate (1 %) and behenate (1 %).

The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine

Streptodornase is a DNase that is often present as in the streptokinase preparations as an impurity.

ACOD1 Protein, Human, Recombinant (His) is expressed in E.

Protease whose physiological substrate is not yet known.

Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway.

B3GNT6 belongs to the glycosyltransferase 31 family.

Phospholipase A2, liquid, food-grade, is an enzymatic product derived from natural sources, typically microbial or animal tissues, utilized in various lipid modification processes. This enzyme specifically targets phospholipids, catalyzing the hydrolysis of the sn-2 ester bond, which releases free fatty acids and lysophospholipids. Its precise and efficient mode of action allows for targeted alterations in the lipid structure, offering potential benefits in modifying texture, flavor, and stability of food products. In food technology and research, Phospholipase A2 is employed to enhance the processing and quality of a wide array of food items. Its applications include the modification of fats in dairy products, improvement of dough properties in baking, and the enhancement of oil extraction and refining processes. By breaking down specific phospholipids, this enzyme helps in reducing rancidity and improving the emulsification properties of food products, thereby extending shelf life and optimizing texture in end products. Its specificity and effectiveness make it a valuable tool for scientists exploring innovative solutions in food processing and quality improvement.

Granzyme K/GZMK Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of

CARKL, also known as SHPK, is a nonprotein kinase of glucose metabolism.

Superoxide Dismutase (SOD2) belongs to the iron/manganese superoxide dismutase family.

Amidase (EC 3.5.1.4) is a hydrolase acting on carbon-nitrogen bonds in linear amides and can be used in the hydrolysis of amides to acids. Amidase 02 is of bacterial origin (R. erythropolis and has been produced in E.coli).

Spermine Oxidase Protein, Mouse, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is Q99K82.

A trypsin product which can be used as a standard in proteomics procedures such as mass spectrometry. Trypsin (EC 3.4.21.4) is a protease that hydrolyses proteins by cleaving the peptide bond at the carboxyl side of the positively charged amino acid (Lysine or Arginine). Trypsin belongs to a family of serine proteases, as it has a serine in its active site. Trypsin can be inhibited by using trypsin inhibitor Alpha 1 Antitrypsin.

Participates in a DNA-damage check-point.

Mitogen-activated protein kinase 9 (MAPK9), also well known as c-Jun N-terminal kinase (JNK2), is a member of the MAP kinase subfamily belonging to the protein